Molecular Study of 16SrRNAGene in Enterobacteriaceaeisolated from Iraqi Patients

Abstract

This study included the collection of 204 clinical and non-clinical samples from Ibn-Al-Baladi childbirth Hospital, Al-Yarmouk Teaching Hospital and Imam Ali Hospital in Baghdad, from both genders of different ages. The collected samples were distributed according to the collection source (urine, wounds, burns, feces, Tigris River water in Baghdad and soil samples). A total of isolates of Escherichia coli (48.43 %), 15 isolates Klebsiella pneumoniae (23.43 %), 10 isolates of Enterobacter cloacae (15.62 %) and 8 isolates of proteus mirabilis (12.50 %) were isolated and identified based on microscopic culture, conventional methods, VITEK 2 and molecular identification of 16 SrRNA gene. Our investigations indicated that Escherichia coli and Klebsiella pneumoniae species represent the most frequent isolates, whereas both Enterobacter cloacae and Proteus mirabilis were the less frequent species. The results of the analysis of the sequencing of selected 21 isolates were deposited in the National Center for Biotechnology Information (NCBI) belong to the four species (From

Detection of acquired resistance genes for vancomycin in local Iraqi isolates of Klebsiella pneumoniae

“…As mentioned in table No.1 ,the samples presented by wounds (33) , urine (60), stool (45) and burns (36) of a total positive samples not exceed 174 Investigation of Klebseilla pneumonia cultures were confirmed by Vit.2 compact system after conducting the traditional methods Tab.1 represents the distribution results in various positive samples and isolates, whereas the percentage of positive samples and the clinical sample were as follows, urine 34.4%, stool 25.86%, wound 18.96% and burns 20.68%. Also, the diagnosis of isolates was confirmed by polymerase chain reaction technology using the 16SrRNA gene, as well as the results of the analysis of the sequences of this gene, which were previously published (Mahmood et al, 2017).…”