Molecular identification of cellulase-producing thermophilic fungi isolated from Sungai Pinang hot spring, Riau Province, Indonesia

Saryono, Saryono; Novianty, Riryn; Suraya, Nabella; PISKA, FINNA; Devi, Silvera; Pratiwi, Nova Wahyu; Ardhi, Aulia

doi:10.13057/biodiv/d230333

Cited by 5 publications

(2 citation statements)

References 31 publications

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“…Results showed that the wastewater sample had a thorium content of 5.50 ppm, temperature of 26.3°C, and pH of 2.4, indicating the possibility of microbial growth, particularly fungi. Previous studies have shown that fungi can survive in various extreme conditions, such as hot springs, hypersaline environments, heavy metal/radionuclides contaminated sites, textile/ industrial wastewater, and others (Tkavc et al 2017;Sanjaya et al 2021;Ezeonuegbu et al 2022;Lira et al 2022;Saryono et al 2022;Wingfield et al 2023).…”

Section: Isolation and Identification Of Indigenous Fungi From Zircon...mentioning

confidence: 99%

Indigenous filamentous fungi isolated from zirconia processing wastewater as a potential biosorbent for aqueous thorium(IV) ions

YAZID,

BASTIANUDIN,

OCTAVIA

et al. 2024

Biodiversitas

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Abstract. Yazid M, Bastianudin A, Octavia B, Putra TD, Rachmani LD, Putri KNA. 2023. Indigenous filamentous fungi isolated from zirconia processing wastewater as a potential biosorbent for aqueous thorium(IV) ions. Biodiversitas 24: 6825-6835. This research focused on exploring indigenous thorium-tolerant fungal isolated from zirconia processing wastewater as a biosorbent for thorium. The objective of present study was to isolate, purify, identify, and analyze the selected capabilities of indigenous fungal biomass in adsorbing thorium from aqueous solution, with the aim of obtaining valuable technical data for its application as a bioremediation agent for technologically enhanced naturally occurring radioactive material (TENORM) wastewater containing thorium. The results showed that total five indigenous fungal isolates, namely two isolates of Penicillium sp. (T2, T4), Rhizoctonia sp. (T3), Mycelia sterilia (T5), and Aspergillus sp. (T6) were isolated from the samples. The result of tolerance index values revealed that two isolates i.e., Penicillium sp. (T2), Penicillium sp. (T4) and a Mycelia sterilia (T5) exhibited high tolerance, while Rhizoctonia sp. (T3) and Aspergillus sp. (T6) showed very high tolerance to thorium. Consequently, Rhizoctonia sp. (T3) was selected for the biosorption study based on the highest tolerance index value obtained. The optimal pH for biosorption was found to be 4 with 30 minutes of contact time and an 80°C temperature. The optimal biosorption efficiency with initial concentrations of 50 and 100 ppm was 97.53 and 90.88%, respectively. In conclusion, based on the obtained technical data, the Rhizoctonia sp. (T3) biomass shows significant potential as a bioremediation agent for wastewater containing thorium.

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Section: Isolation and Identification Of Indigenous Fungi From Zircon...mentioning

confidence: 99%

Indigenous filamentous fungi isolated from zirconia processing wastewater as a potential biosorbent for aqueous thorium(IV) ions

YAZID,

BASTIANUDIN,

OCTAVIA

et al. 2024

Biodiversitas

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“…The mixture was vortexed and boiled for 15 min at 100 • C. Next, 1 mL of Nelson reagent was added, and the absorbance was measured at 520 nm. Concentrations of reducing sugars were determined based on a standard curve of xylose concentrations and following the methodology of [29].…”

Section: Assessment Of Reducing Sugarsmentioning

confidence: 99%

Production of Xylanase by Trichoderma Species Growing on Olive Mill Pomace and Barley Bran in a Packed-Bed Bioreactor

Alananbeh,

Alkfoof,

Muhaidat

et al. 2024

JoF

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Xylanases are hydrolytic enzymes that have tremendous applications in different sectors of life, but the high cost of their production has limited their use. One solution to reduce costs and enhance xylanase production is the use of agro-wastes as a substrate in fungal cultures. In this study, olive mill pomace (OMP) and barley bran (BB) were used as carbon sources and possible inducers of xylanase production by three species of Trichoderma (atroviride, harzianum, and longibrachiatum), one major xylanase producer. The experiments were conducted under a solid-state fermentation system (SSF) in flask cultures and a packed-bed bioreactor. Cultures of OMP and BB were optimized by examining different ratios of OMP and BB, varied particle sizes, and inoculum size for the three species of Trichoderma. The ratio of 8:2 OMP and BB yielded the highest xylanase activity, with a particle size of 1 mm at 29 °C and an inoculum size of 1 × 107 spores/mL. Studying the time profile of the process revealed that xylanase activity was highest after seven days of incubation in flask SSF cultures (1.779 U/mL) and after three days in a packed-bed bioreactor (1.828 U/mL). The maximum percentage of OMP degradation recorded was about 15% in the cultures of T. harzianum flask SSF cultures, compared to about 11% in T. longibrachiatum bioreactor cultures. Ammonium sulfate precipitation and dialysis experiments showed that Xylane enzyme activity ranged from 0.274 U/mL in T. harzianum to 0.837 U/mL in T. atroviride when crude extract was used, with the highest activity (0.628 U/mL) at 60% saturation. Xylose was the main sugar released in all purified fractions, with the G-50 and G-75 fractions showing the maximum units of xylanase.

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Biodiversity of Fruit Crops and Utilization in Food and Nutritional Security

Mansyah,

Budiyanti,

Hadiati

et al. 2024

Sustainable Development and Biodiversity

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Molecular identification of cellulase-producing thermophilic fungi isolated from Sungai Pinang hot spring, Riau Province, Indonesia

Cited by 5 publications

References 31 publications

Indigenous filamentous fungi isolated from zirconia processing wastewater as a potential biosorbent for aqueous thorium(IV) ions

Indigenous filamentous fungi isolated from zirconia processing wastewater as a potential biosorbent for aqueous thorium(IV) ions

Production of Xylanase by Trichoderma Species Growing on Olive Mill Pomace and Barley Bran in a Packed-Bed Bioreactor

Biodiversity of Fruit Crops and Utilization in Food and Nutritional Security

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