Molecular identification and phylogenetic analysis of aCallosciurus notatuscomplete mitogenome from Peninsular Malaysia

Abstract

The mitogenome of a plantain squirrel, Callosciurus notatus, collected from Bukit Tarek Forest Reserve (Extension), Selangor, Malaysia was sequenced using BGISEQ-500RS technology. The 16,582 bp mitogenome consists of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 control region. A phylogenetic and BLASTn analysis against other available datasets showed that the mitogenome matched with 99.49% similarity to a previously published C. notatus mitogenome from Peninsular Malaysia. However, it also diverged by nearly 8% (92.24% match) from a second previously published mitogenome for the same species, sampled in East Kalimantan, Indonesia. This suggests a difference in landscape features between both localities might affect its genetic connectivity.

The first mitochondrial genome data of an old world fruit bat, Cynopterus sphinx from Malaysia

“… 2020 ; Jahari, Mohd Azman, et al. 2020 ). The library was pooled to equimolar and shotgun sequenced on BGISEQ-500 platform in 100 bp paired-end mode (PE100) (BGI, Shenzhen, China).…”

“…Genomic DNA was extracted using Qiagen Blood and Tissue Kit (Qiagen, Valencia, CA). The DNA was later fragmented (300-400bp) using a M220 Focused-ultrasonicator (Covaris, USA) and BGISeq compatible shotgun sequencing library was built using the Blunt-End-Single-Tube (BEST) library protocol (Carøe et al 2018;Jahari, Abdul Malik, et al 2020;Jahari, Mohd Azman, et al 2020). The library was pooled to equimolar and shotgun sequenced on BGISEQ-500 platform in 100 bp paired-end mode (PE100) (BGI, Shenzhen, China).…”

Characterization of the mitogenomes of long-tailed giant rat, Leopoldamys sabanus and a comparative analysis with other Leopoldamys species

“…( 2020 ) and Jahari, Mohd Azman, et al. ( 2020 ). Both mitogenomes of L. sabanus (Genbank accession no.…”