Characterization of the mitogenomes of long-tailed giant rat, <i>Leopoldamys sabanus</i> and a comparative analysis with other <i>Leopoldamys</i> species

Jahari, Puteri Nur Syahzanani; Azman, Shahfiz Mohd; Munian, Kaviarasu; Ruzman, N. H. Ahmad; Shamsir, Mohd Shahir; Richter, Stine Raith; Salleh, Faezah Mohd

doi:10.1080/23802359.2021.1872433

Cited by 1 publication

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“…A total of 10,102,764 raw reads were obtained and firstly verified using the FastQC program for quality assessment ( https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ ). Next, the raw reads were trimmed for sequencing adapters, low-quality bases as well as Ns [ 6 , 7 ] using AdapterRemoval v2.3.2 [8] . Sequences with quality score of 20 and above were retained.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

The complete mitochondrial genome data of the Common Rose butterfly, Pachliopta aristolochiae (Lepidoptera, Papilionoidea, Papilionidae) from Malaysia

et al. 2022

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Here, we present the complete mitochondrial genome of Pachliopta aristolochiae , a Common Rose butterfly from Malaysia. The sequence was generated using Illumina NovaSeq 6000 sequencing platform. The mitogenome is 15,235bp long, consisting of 13 protein-coding genes, 22 transfer RNAs, two ribosomal RNAs, and two D-loop regions. The total base composition was (81.6%), with A (39.3%), T (42.3%), C (11.0%) and G (7.3%). The gene order of the three tRNAs was trnM-trnI-trnQ , which differs from the ancestral insect gene order trnI-trnQ-trnM . Phylogenetic tree analysis revealed that the sequenced Pachliopta aristolochiae in this data is closely related to Losaria neptunus (NC 037868), with highly supported ML and BI analysis. The data presented in this work can provide useful resources for other researchers to study deeper into the phylogenetic relationships of Lepidoptera and the diversification of the Pachliopta species. Also, as one of the bioindicator species, this data can be used to assess environmental changes in the terrestrial and aquatic ecosystem via enviromental DNA approahes. The mitogenome of Pachliopta aristolochiae is available in GenBank under the accession number MZ781228.

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Section: Experimental Design Materials and Methodsmentioning

confidence: 99%