The first mitochondrial genome data of an old world fruit bat, <i>Cynopterus sphinx</i> from Malaysia

Jahari, Puteri Nur Syahzanani; Azman, Shahfiz Mohd; Munian, Kaviarasu; Zakaria, Nur Alwani; Shamsir, Mohd Shahir; Richter, Stine Raith; Salleh, Faezah Mohd

doi:10.1080/23802359.2020.1846472

Cited by 1 publication

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“…Further annotation of individual mitochondrial genes by MITOS determined that almost all vertebrate mitochondrial genes were present on the assembled mitogenome, except for trnT. The length (17,382 bases) and GC content (41.48%) of the assembled mitogenome were similar and hence concordant with other published fruit bat mitogenomes ( Jahari et al 2020 , Jahari et al 2021 , Yue 2019 ). These steps and resulting statistics confirm that a true, high-quality bat mitogenome has been assembled from the sequence reads and the identity of this assembled mitogenome can be attributed to Cynopterus sp..…”

Section: Discussionsupporting

confidence: 73%

The first complete mitochondrial genome sequence of Cynopterus brachyotis (Chiroptera, Pteropodidae) from the Philippines

Gaite¹,

Aala²,

Bacus³

et al. 2022

BDJ

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The technical limitations of capillary sequencing in providing insights on phylogeny have been greatly aided in recent years by the implementation of next generation sequencing platforms which can generate whole mitochondrial genome (mitogenome) sequences. In this study, enriched mitochondrial DNA of Cynopterus brachyotis from Mindanao, Philippines was sequenced using the Illumina MiSeq platform. A total of 653,967 clean paired-end reads was assembled using a MIRA-MITObim pipeline, resulting in a consensus mitogenome sequence length of 17,382 bases and a GC content of 41.48%, which is consistent with other published mitogenomes in fruit bats. The assembled C. brachyotis mitogenome was annotated using the MITOS online server and was able to resolve all mitochondrial genes, except for one transfer RNA gene (trnT) which may be further resolved by additional capillary sequencing of the region. Sequence analysis showed that the Philippine C. brachyotis is only 90%-91% homologous with other Cynopterus spp., based on its full mitogenome sequence. Phylogenetic analysis of fruit bat mitogenomes, deposited in online repositories, revealed that the Philippine C. brachyotis in this study has diverged from Asian Cynopterus, namely Cynopterus brachyotis and Cynopterus sphinx from other parts of Asia (100% bootstrap support) with the latter two forming a separate clade. This divergence at the species level was consistent with phylogentic inference using cytochrome oxidase 1 (CO1) and cytochrome B (cytb) gene markers. Our results strengthen the previously reported hypothesis that the Cynopterus cf. brachyotis in the Philippines is distinct from its Asian counterparts and should be, therefore, treated as a new species.

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