Molecular identification and biodegradation of 3-chloropropionic acid (3CP) by filamentous fungi-Mucor and Trichoderma species isolated from UTM agricultural land

Parvizpour, Sepideh; Hamid, Tengku Aizan; Huyop, Fahrul

doi:10.21161/mjm.40412

Cited by 3 publications

(4 citation statements)

References 8 publications

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“…Trichoderma species are the most ubiquitous saprophytic component, sustain for longer time even in increased pollution conditions [8,15]. 3CP degradation potential was previously described in Trichoderma sp.…”

Section: Discussionmentioning

confidence: 99%

“…Hamid [8] reported the dehalogenation of 3CP and the synthesis of 3-hydroxypropionic acid (3HP) as the by-product. 3HP is non-toxic carbon source which can be easily absorbed and utilized [4].…”

Section: Discussionmentioning

confidence: 99%

“…Samples were stored at 4°C in a refrigerator for further use. Samples were prepared as described by Parvizpour, Hamid [8].One gram of soil sample was serially diluted and spread on the potato dextrose agar plates supplemented with 10mM of 3CP.…”

Section: Sample Collection and Isolationmentioning

confidence: 99%

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3-Chloropropionic Acid (3cp) Degradation and Production of Propionic Acid by Newly Isolated Fungus Trichoderma Sp. Mf1

Edbeib¹

2020

International Journal of Life Sciences and Biotechnology

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Biologically synthesized chemicals have drawn a growing interest recently. The main objectives of the study are to isolate 3-chloropropionic acid (3CP) degrading fungus and to examine its ability to produce Propionic Acid (PA) as a by-product. 3CP is widely used in many pesticide and herbicide synthesis due to biocidal properties but it is toxic and recalcitrant to be removed from the biosphere. Bioremediation approaches through dehalogenation have promised removal of these xenobiotics. Fungi are selected due to its broad enzymatic capacities which is not limited like bacteria. In this research, several fungal isolates have been purified, among the isolates, strain designated as MF1 has shown greater potential in 3CP degradation. Using morphological and molecular approaches, MF1 was identified as Trichoderma sp. Amplification of ITS genome region (Accession No. MT126695) revealed that the MF1 isolate had 99% identity to Trichoderma asperellum strain AF14. Strain MF1 growth rate is 1.42cm/day on solid medium and it was able to produce biomass up to 0.855g/L in liquid minimal medium supplemented with 10mM 3CP. Whereas the growth in control medium containing 1% glucose has resulted in biomass of 1.814g/L. 90.32% of 10mM 3CP were successfully de-chlorinated within 20 days. Confirmed by HPLC, PA was the major product of dehalogenation with highest concentration of 2.72mM at day 10. Presented data can be used for the designing of by-product extraction. Dehalogenation of 3CP by Trichoderma asperellum MF1 have not only been successfully removed xenobiotic pollutant but also have open for new strategy on synthesis of industrial required chemicals.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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3-Chloropropionic Acid (3cp) Degradation and Production of Propionic Acid by Newly Isolated Fungus Trichoderma Sp. Mf1

Edbeib¹

2020

International Journal of Life Sciences and Biotechnology

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“…These enzymes differ in their optimum pH for activity, size and subunit structure, electrophoretic mobility under nondenaturing conditions, and substrate specificity [ 11 , 12 ]. Currently, the haloacid dehalogenase enzymes from both bacterial and fungal sources receive greater attention because of their potential use in biotechnological applications in the bioremediation of haloacid environmental pollutants [ 13 , 14 ]. Many haloacid based xenobiotic compounds that are difficult to eliminate are being abundantly dispersed in the environment causing hazardous health concerns.…”

Section: Introductionmentioning

confidence: 99%

In Silico Phylogenetic Analysis and Molecular Modelling Study of 2-Haloalkanoic Acid Dehalogenase Enzymes from Bacterial and Fungal Origin

Satpathy

Konkimalla

Ratha

2016

Advances in Bioinformatics

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2-Haloalkanoic acid dehalogenase enzymes have broad range of applications, starting from bioremediation to chemical synthesis of useful compounds that are widely distributed in fungi and bacteria. In the present study, a total of 81 full-length protein sequences of 2-haloalkanoic acid dehalogenase from bacteria and fungi were retrieved from NCBI database. Sequence analysis such as multiple sequence alignment (MSA), conserved motif identification, computation of amino acid composition, and phylogenetic tree construction were performed on these primary sequences. From MSA analysis, it was observed that the sequences share conserved lysine (K) and aspartate (D) residues in them. Also, phylogenetic tree indicated a subcluster comprised of both fungal and bacterial species. Due to nonavailability of experimental 3D structure for fungal 2-haloalkanoic acid dehalogenase in the PDB, molecular modelling study was performed for both fungal and bacterial sources of enzymes present in the subcluster. Further structural analysis revealed a common evolutionary topology shared between both fungal and bacterial enzymes. Studies on the buried amino acids showed highly conserved Leu and Ser in the core, despite variation in their amino acid percentage. Additionally, a surface exposed tryptophan was conserved in all of these selected models.

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A novel putative non-ligninolytic dehalogenase activity for 3-chloropropionic acid (3CP) utilization by Trichoderma asperellum strain SD1

Selvamani¹,

Wahab²,

Huyop³

2015

MJM

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Aims: Trichoderma asperellum strain SD1 grows on 3-chloropropionic acid (3CP), a β-haloalkanoic acid, and produces a putative extracellular dehalogenase that can degrade this acid. Here we further characterized the fungal enzyme system responsible for biodegradation of 3CP. Methodology and results: The primary qualification of the ligninolytic potential in T. asperellum strain SD1 was performed using guaiacol oxidation. When strain SD1 was grown in liquid minimal medium with the presence of 3CP as the sole carbon source, no lignin peroxidase, manganese peroxidase, or laccase activity was detected. The ligninolytic condition was achieved only in the presence of glucose or when guaiacol was present as an inducer. Under nonligninolytic conditions, 3CP was utilized by strain SD1. Therefore, 3CP was utilized under ligninolytic conditions as well as under non-ligninolytic conditions, suggesting that extracellular peroxidases and laccase are not involved in the degradation of 3CP by T. asperellum strain SD1. Conclusion, significance, and Impact of study: Very few studies have explained the degradation of β-chlorosubstituted haloalkanoic acids such as 3CP by dehalogenases. This is the first report to identify a novel putative βhaloacid dehalogenase that degrades 3CP under ligninolytic and non-ligninolytic conditions. T. asperellum strain SD1, thus has the potential in the development of dehalogenating enzymes for industrial biocatalytic processes, in future.

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Molecular identification and biodegradation of 3-chloropropionic acid (3CP) by filamentous fungi-Mucor and Trichoderma species isolated from UTM agricultural land

Cited by 3 publications

References 8 publications

3-Chloropropionic Acid (3cp) Degradation and Production of Propionic Acid by Newly Isolated Fungus Trichoderma Sp. Mf1

3-Chloropropionic Acid (3cp) Degradation and Production of Propionic Acid by Newly Isolated Fungus Trichoderma Sp. Mf1

In Silico Phylogenetic Analysis and Molecular Modelling Study of 2-Haloalkanoic Acid Dehalogenase Enzymes from Bacterial and Fungal Origin

A novel putative non-ligninolytic dehalogenase activity for 3-chloropropionic acid (3CP) utilization by Trichoderma asperellum strain SD1

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