A novel putative non-ligninolytic dehalogenase activity for 3-chloropropionic acid (3CP) utilization by Trichoderma asperellum strain SD1

Abstract

Aims: Trichoderma asperellum strain SD1 grows on 3-chloropropionic acid (3CP), a β-haloalkanoic acid, and produces a putative extracellular dehalogenase that can degrade this acid. Here we further characterized the fungal enzyme system responsible for biodegradation of 3CP. Methodology and results: The primary qualification of the ligninolytic potential in T. asperellum strain SD1 was performed using guaiacol oxidation. When strain SD1 was grown in liquid minimal medium with the presence of 3CP as the sole carbon source, no lignin peroxidase, manganese peroxidase, or laccase activity was detected. The ligninolytic condition was achieved only in the presence of glucose or when guaiacol was present as an inducer. Under nonligninolytic conditions, 3CP was utilized by strain SD1. Therefore, 3CP was utilized under ligninolytic conditions as well as under non-ligninolytic conditions, suggesting that extracellular peroxidases and laccase are not involved in the degradation of 3CP by T. asperellum strain SD1. Conclusion, significance, and Impact of study: Very few studies have explained the degradation of β-chlorosubstituted haloalkanoic acids such as 3CP by dehalogenases. This is the first report to identify a novel putative βhaloacid dehalogenase that degrades 3CP under ligninolytic and non-ligninolytic conditions. T. asperellum strain SD1, thus has the potential in the development of dehalogenating enzymes for industrial biocatalytic processes, in future.

Isolation and Characterization of a Novel Bacterium from the Marine Environment for Trichloroacetic Acid Bioremediation

“…The isolated strain was reported to have outstanding performance in detoxifying L-2-haloacid. Selvamani et al [45] characterized deploying Trichoderma asperellum SD1 to degrade 3-chloropropionic acid in the terrestrial ecosystem. Bagherbaigi et al [46] used Arthrobacter sp.…”

Isolation and characterization of a biodegrading 3-chloropropionic acid Burkholderia cepacia WH1 isolated from abandoned agricultural land

“…The consistent decline in the concentration of 3CP in the growth media with the increase in the incubation time strongly suggested that Burkholderia cepacia was capable of degrading and utilizing the substance as a carbon and energy source. Hence, it is suggested that the ability of Burkholderia cepacia to utilize 3CP in this study may be associated with the existence of a cluster of enzymatic reaction or reactions that favoured the dehalogenation of 3CP (Selvamani et al, 2015). The findings in this study also suggest that the b-dehalogenase in Burkholderia cepacia may have an important role in the degradation of b-chlorinated xenobiotics in the environment.…”

The overexpression of one single cbh gene making Trichoderma asperellum T-1 a better cellulase producer

“…Most importantly, this strain can ferment the natural materials without pretreatment, showing a great potential in the degradation of natural waste lignocellulosic material (Wang et al 2015;Wang et al 2017). Studies about T. asperellum have reported its potential in glycoside hydrolase (GH) production, and those hydrolases can degrade the carbohydrates in the plant cell walls to release bioactive polymers, proteins, and simple sugars (Selvamani et al 2015).…”