Molecular genetics of the swine major histocompatibility complex, the SLA complex

Lunney, Joan K.; Ho, Chak-Sum; Wysocki, Michał; Smith, Douglas M.

doi:10.1016/j.dci.2008.07.002

Cited by 169 publications

(154 citation statements)

References 165 publications

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“…Additional analyses of other exon regions may reveal further genetic variation, which should be examined in future studies. Table 2 shows that exon 2 displayed an intermediate level of polymorphism, while exons 3 and 4 showed high polymorphism, which agreed with the results of Lunney et al (2009) and Yang et al (2013). Genetic diversity is essential for the preservation of adaptive potential of species and improvement of production potential of highly selected breeds.…”

Section: Discussionsupporting

confidence: 76%

“…SLA class II includes heterodimeric proteins composed of 2 non-covalently linked a (34 kDa) and b (29 kDa) chains with a chain coded by the DRA and DQA genes (Lunney et al, 2009;Takeshima et al, 2009). The a and b domains together form the peptide-binding region, where molecular pockets influence the binding of foreign peptides and subsequent immune responses (Vander et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

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Polymorphism and haplotype analyses of swine leukocyte antigen DQA exons 2, 3, 4, and their associations with piglet diarrhea in Chinese native pig

et al. 2015

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ABSTRACT. In this study, 290 Chinese native Yantai black pig piglets were investigated to identify gene polymorphisms, for haplotype reconstruction, and to determine the association between piglet diarrhea and swine leukocyte antigen (SLA) class II DQA exons 2, 3, and 4 by polymerase chain reaction-single stranded conformational polymorphism and cloning sequencing. The results showed that the 5, 8, and 7 genotypes were identified from SLA-DQA exon 2, 3, and 4, respectively, based on the single-stranded conformational polymorphism banding patterns and found a novel allele D in exon 2 and 2 novel mutational sites of allele C (c.4828T>C) and allele F (c.4617T>C) in exon 3. Polymorphism information content testing showed that exon 2 was moderately polymorphic and that exons-3 and -4 loci were highly polymorphic. The piglet diarrhea scores for genotypes AB (1.40 ± 0.14) and AC (1.54 ± 0.17) in exon 2, AA (1.22 ± 0.32), BC (1.72 ± 0.13), DD (2015) (1.67 ± 0.35), and CF (1.22 ± 0.45) in exon 3, and AD (2.35 ± 0.25) in exon 4 were significantly higher than those for the other genotypes (P ≤ 0.05) in DQA exons. There were 14 reconstructed haplotypes in the 3 exons from 290 individuals and Hap12 may be the diarrhea-resistant gene. Haplotype distribution was extremely uneven, and the SLA-DQA gene showed genetic linkage. In this study, we identified molecular genetic markers and provided a theoretical foundation for future pig anti-disease resistance breeding.

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Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Polymorphism and haplotype analyses of swine leukocyte antigen DQA exons 2, 3, 4, and their associations with piglet diarrhea in Chinese native pig

et al. 2015

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“…The designation of allele-specific primer sets were conducted with the principle that the 3' end of one or both primers covered polymorphic sites unique to their corresponding allele, and that each allele-specific primer pair was located in the same exon, generally in exon 2 or exon 3, which showed high polymorphism (Lunney et al, 2009). Two SLA-DRA, two SLA-DRB1, four SLA-DQA, and four SLA-DQB1 primer pairs were designed.…”

Section: Alleles Sequence Specific Primer and Pcr-sspmentioning

confidence: 99%

Swine leukocyte antigen class II genes (SLA-DRA, SLA-DRB1, SLA-DQA, SLA-DQB1) polymorphism and genotyping in Guizhou minipigs

Liu¹,

Xia²,

Xin³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The swine leukocyte antigen (SLA) complex harbors highly polymorphic gene clusters encoding glycoproteins that are involved in responses to vaccines, infectious disease, and production performance. Pigs with well-defined SLA class II genes are useful for the study of disease, immunology, and vaccines. In this study, we analyzed four SLA class II genes (SLA-DRA, SLA-DRB1, SLA-DQA, SLA-DQB1) in 22 founder Guizhou minipigs using a sequence-based typing method. Twelve alleles were detected, compared with the SLA class II allele sequences in the GenBank, and one of twelve alleles was found to be novel in Guizhou minipigs. There are four SLA II haplotypes, and one of them has been previously reported in Meishan pigs. Furthermore, based on sequence information of these alleles, we developed a simple 15257 SLA class II genes in Guizhou minipigs ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 15256-15266 (2015) SLA typing method implemented to SLA-typing for unknown offspring of Guizhou minipigs, relying on designed twelve sequence specific primers that could discriminate between each other. According to the combination of sequence-based typing and PCR-SSP, we were able to rapidly check SLA typing of Guizhou breeding stock and identified four SLA haplotypes in the herd. Therefore, SLA-defined Guizhou minipigs will be useful as animal models for xenotransplantation and immunological research.

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“…The β2 domain of MHC class II molecules binds to the CD4 molecule on the surface of T cells (Vignali et al, 1992;Bondinas et al, 2007), which in turn contributes to the proliferation of helper T lymphocytes. Although exon 3 participates in encoding the β2 domain (Scott et al, 1991;Lunney et al, 2009), polymorphisms in this region of MHC class II genes have rarely been detected in pigs. As a main member of MHC class II molecules, DQ is also capable of interacting with T lymphocytes.…”

Section: Introductionmentioning

confidence: 99%

Expression Profile and PorcinDQB1Gene Association Analysis of the with Peripheral Blood T Lymphocyte Subsets

Cai

Liu

et al. 2015

Italian Journal of Animal Science

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Major histocompatibility complex (MHC) class II molecules play an important role in immunology by presenting antigens to T lymphocytes. As a key member of the MHC class II gene family, the DQB1 gene is involved in interacting with T cells in immune reactions. This study was designed to screen variations in exon 3 of the DQB1 gene in Large White, Landrace and Songliao Black pigs, and to investigate the association between DQB1 gene polymorphisms and peripheral blood T lymphocyte subsets in Large White samples. In addition, the spatial transcription profile of the DQB1 gene was examined, and the effect of gene polymorphisms on its mRNA level was further analyzed. One missense mutation in exon 3 of the DQB1 gene was first identified by DNA pool sequencing, and samples were genotyped by using the polymerase chain reactionrestriction fragment length polymorphism method. Statistical analysis indicated that the DQB1 genotype was significantly associated with CD4＋ CD8 -, CD4 -CD8 ＋ and CD4 ＋ /CD8 ＋ indexes (P<0.05). Analysis of mRNA distribution showed that DQB1 was predominantly expressed in the spleen. However, no significant differences in splenic DQB1 mRNA levels of pigs with different genotypes were observed (P>0.05). These results suggest that the DQB1 gene may be a promising candidate gene for porcine disease resistance.

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Molecular genetics of the swine major histocompatibility complex, the SLA complex

Cited by 169 publications

References 165 publications

Polymorphism and haplotype analyses of swine leukocyte antigen DQA exons 2, 3, 4, and their associations with piglet diarrhea in Chinese native pig

Polymorphism and haplotype analyses of swine leukocyte antigen DQA exons 2, 3, 4, and their associations with piglet diarrhea in Chinese native pig

Swine leukocyte antigen class II genes (SLA-DRA, SLA-DRB1, SLA-DQA, SLA-DQB1) polymorphism and genotyping in Guizhou minipigs

Expression Profile and PorcinDQB1Gene Association Analysis of the with Peripheral Blood T Lymphocyte Subsets

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