Expression Profile and PorcinDQB1Gene Association Analysis of the with Peripheral Blood T Lymphocyte Subsets

Abstract

Major histocompatibility complex (MHC) class II molecules play an important role in immunology by presenting antigens to T lymphocytes. As a key member of the MHC class II gene family, the DQB1 gene is involved in interacting with T cells in immune reactions. This study was designed to screen variations in exon 3 of the DQB1 gene in Large White, Landrace and Songliao Black pigs, and to investigate the association between DQB1 gene polymorphisms and peripheral blood T lymphocyte subsets in Large White samples. In addition, the spatial transcription profile of the DQB1 gene was examined, and the effect of gene polymorphisms on its mRNA level was further analyzed. One missense mutation in exon 3 of the DQB1 gene was first identified by DNA pool sequencing, and samples were genotyped by using the polymerase chain reactionrestriction fragment length polymorphism method. Statistical analysis indicated that the DQB1 genotype was significantly associated with CD4＋ CD8 -, CD4 -CD8 ＋ and CD4 ＋ /CD8 ＋ indexes (P<0.05). Analysis of mRNA distribution showed that DQB1 was predominantly expressed in the spleen. However, no significant differences in splenic DQB1 mRNA levels of pigs with different genotypes were observed (P>0.05). These results suggest that the DQB1 gene may be a promising candidate gene for porcine disease resistance.

Quantitative Proteomic Analysis of Tibetan Pig Livers at Different Altitudes

“…Two SLA-DQ proteins (F6PX38 and Q7YQ94) were expressed in high abundance in the livers of SNT and LZT, while two SLA-DR proteins (A0A286ZUJ9 and Q85ZW4) were less abundant in the livers of SNT and LZT. Previous studies reported that SLA-DQB1 is able to participate in the interaction with T cells in the immune response and may be a candidate gene related to disease resistance in pigs [ 41 ], while significant up-regulation of SLA-DQA in piglet organs was able to reduce transmembrane signaling of lipopolysaccharide from E. coli F18 and enhance humoral and cellular immunity [ 42 ]. In addition, SLA-DR is a key MHC II molecule involved in antigen presentation and initiation of CD4+ T cell activation, and downregulation of such molecules may lead to delayed host antibody responses [ 43 , 44 ].…”