Swine leukocyte antigen class II genes (SLA-DRA, SLA-DRB1, SLA-DQA, SLA-DQB1) polymorphism and genotyping in Guizhou minipigs

Abstract

ABSTRACT. The swine leukocyte antigen (SLA) complex harbors highly polymorphic gene clusters encoding glycoproteins that are involved in responses to vaccines, infectious disease, and production performance. Pigs with well-defined SLA class II genes are useful for the study of disease, immunology, and vaccines. In this study, we analyzed four SLA class II genes (SLA-DRA, SLA-DRB1, SLA-DQA, SLA-DQB1) in 22 founder Guizhou minipigs using a sequence-based typing method. Twelve alleles were detected, compared with the SLA class II allele sequences in the GenBank, and one of twelve alleles was found to be novel in Guizhou minipigs. There are four SLA II haplotypes, and one of them has been previously reported in Meishan pigs. Furthermore, based on sequence information of these alleles, we developed a simple 15257 SLA class II genes in Guizhou minipigs ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 15256-15266 (2015) SLA typing method implemented to SLA-typing for unknown offspring of Guizhou minipigs, relying on designed twelve sequence specific primers that could discriminate between each other. According to the combination of sequence-based typing and PCR-SSP, we were able to rapidly check SLA typing of Guizhou breeding stock and identified four SLA haplotypes in the herd. Therefore, SLA-defined Guizhou minipigs will be useful as animal models for xenotransplantation and immunological research.

Swine Leukocyte Antigen Diversity in Canadian Specific Pathogen-Free Yorkshire and Landrace Pigs

“…No novel class II haplotypes were identified in SPF Yorkshire and Landrace pigs. Hp-0.23 was the most common SLA class II haplotype in this study and was shared by several other pig populations, including Microminipigs, the IU-pig model, Landrace × Yorkshire, Guizhou miniature pigs, Korean native pigs, Big pigs, German purebred Landrace, and Pietrain pigs (17, 26, 31, 32, 34, 35, 38, 39) (Table 4). This suggests that Yorkshire and Landrace pigs may share some common genetic background with these breeds.…”

Transcriptome differences in frontal cortex between wild boar and domesticated pig

“…Six genes encoding immunoglobulin light chain were upregulated in domesticated pigs, namely three immunoglobulin lambda genes ( IGLC , IGLV‐3 , IGLV‐9 ) and three immunoglobulin kappa genes ( IGKC , IGKV‐7 , IGKV‐11 ). SLA is a gene‐dense region and includes class I, II, and III gene clusters located in the centromere spanning chromosome 7 (Liu et al., ). SLA class II genes play multiple roles in the immune response to foreign antigens and to vaccines, such as involvement in the differentiation of myeloid dendritic cells from plasmacytoid or natural interferon producing cells (Summerfield et al., ), controlling infectious disease responses and influencing vaccine specificity and efficacy (Fuertes et al., ).…”

Genetic association of sequence variation in exon 3 of the swine leukocyte antigen-DQA gene with piglet diarrhea in Large White, Landrace, and Duroc piglets

“…Our findings extend the current knowledge of SLA-DQA, and will stimulate further biomedical research and diarrhea-resistant pig-breeding programs. Intragenic recombination studies have clear advantages over genedisease association studies when working on single-locus alleles (Liu et al, 2015a), and nucleotide sequence variations in other exon coding regions of SLA-DQA may also contribute to diarrhea in piglets, or might link to other SLA class II genes. Further work on obtaining full-length cDNA sequences of other SLA molecules and validating the linkage between these class II genes and diarrhea is required.…”

“…Swine leukocyte antigen (SLA) is a member of an immune response-related multigene family, spans the centromere of swine chromosome 7, and consists of class I, II, and III genes that participate in combating the invasion of pathogens and infectious diseases (Renard et al, 2006). The roles of SLA-encoded genes in the development and control of immunological diseases are well established, particularly those of SLA II genes (Lunney et al, 2009;Shinkai et al, 2012;Ye et al, 2012;Liu et al, 2015a). Previous studies on the molecular characterization of SLA II genes have mainly focused on the functional region of exon 2, as amino acid substitutions here are known to play crucial roles in the formation of functional conformations and in processing foreign peptides (Luetkemeier et al, 2009).…”