Molecular evidence supporting the confirmation of Maracuja mosaic virus as a species of the genus Tobamovirus and production of an infectious cDNA transcript

Abstract

The complete genome sequence of maracuja mosaic virus (MarMV) was determined and analyzed. The full MarMV genome consisted of 6794 nucleotides, and this is the largest genome size among known tobamoviruses. The MarMV genome RNA contained four open reading frames (ORFs) coding for proteins of M(r) 126, 181, 34 and 18 kDa from the 5' to 3' end, respectively. The lengths of the 5' nontranslated region (NTR) and the 3' NTR were 54 and 177 nucleotides, respectively. Phylogenetic tree analysis revealed that these MarMV-encoded proteins are related to members of the Malvaceae- and Cucurbitaceae-infecting tobamoviruses. MarMV is different from other tobamoviruses and forms a new Passifloraceae-infecting subgroup. Western blot analysis showed that MarMV cross-reacted strongly with antibodies against Kyuri green mottle mosaic virus and Hibiscus latent Singapore virus. Synthesized capped transcripts from full-length cDNA of MarMV were infectious. These data clearly indicate that MarMV belongs to a separate species of the genus Tobamovirus.

The complete genome sequence, occurrence and host range of Tomato mottle mosaic virus Chinese isolate

“…Song et al [15] suggested the existence of a sixth subgroup in the genus Tobamovirus isolated from the Passifloraceae, based on the phylogenetic analysis of the four tobamovirus proteins. Here, according to the phylogenetic analyses and the hosts from which the viruses were originally isolated, the presently available 32 tobamoviruses could be divided into at least eight subgroups, Solanaceae-, Brassicaceae-, Cactaceae-, Apocynaceae-, Cucurbitaceae-, Malvaceae-, Leguminosae-, and Passifloraceae-infecting subgroups based on both the complete nucleotide sequences and 126 kDa, 54 kDa, MP and CP amino acid sequences.…”

“…Min and co-workers [14] proposed that tobamoviruses could be divided into at least five subgroups according to the amino acid composition and primary structure of their CPs, and the hosts from which the viruses were originally isolated. Song et al [15] suggested tobamoviruses should be divided into six subgroups based on the phylogenetic analysis of the four tobamovirus proteins with the existence of passifloraceae-infecting subgroup in the genus Tobamovirus .…”

Tobamovirus 3′-Terminal Gene Overlap May be a Mechanism for within-Host Fitness Improvement

“…The OAs of viruses from subgroup 3 are also located in the MP ORF; however, the MP and CP ORFs overlap by 77 nt, and members of this subgroup are able to infect both Solanaceae and Brassicaceae species. Isolation of new tobamoviruses, such as frangipani mosaic virus (Lim et al, 2010), maracuja mosaic virus (Song et al, 2006), passion fruit mosaic virus (Song and Ryu, 2011), cactus mild mottle virus (Min et al, 2006) and rattail cactus necrosis-associated virus (Kim et al, 2012), along with the complete sequencing of their genomes, showed that MP/CP overlap is not unique to Brassicaceae- infecting tobamoviruses but is also present in Apocynaceae -, Passifloraceae -, and Cactaceae -infecting viruses (Figure 1). …”

Characterization of the complete genome of ribgrass mosaic virus isolated from Plantago major L. from New Zealand and Actinidia spp. from China

“…Tobamoviruses have been classified into different groups based on host range, coat protein structure, serological relationships and genome organization [2,10,11,[16][17][18]21], and their evolution of has been comprehensively reviewed [3,11,13,16,23]. The genus Tobamovirus contains 22 definitive and one tentative species [10] and is divided into three subgroups based on natural host range, genomic organization and phylogenetic clustering [10,11,16,17].…”

“…The genus Tobamovirus contains 22 definitive and one tentative species [10] and is divided into three subgroups based on natural host range, genomic organization and phylogenetic clustering [10,11,16,17]. However some authors propose five subgroups of tobamoviruses, infecting members of the families Brassicaceae, Cactaceae, Cucurbitaceae, Malvaceae, and Solanaceae/Orchidaceae, based on the primary structure of the coat protein, serological specificity and host range [2,18], while Song et al [21] have proposed a sixth subgroup of tobamoviruses infecting members of the family Passifloraceae. A recent analysis of tobamovirus sequences by Adams et al [1] describing the family Virgaviridae has highlighted some incorrectly labelled sequences in that genus.…”