Molecular Evidence of Placental Hypoxia in Preeclampsia

Soleymanlou, Nima; Jurišica, Igor; Nevo, Ori; Ietta, Francesca; Zhang, Xin; Zamudio, Stacy; Post, Martin; Caniggia, Isabella

doi:10.1210/jc.2005-0078

Cited by 336 publications

(280 citation statements)

References 59 publications

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“…Oxygen is one of the key elements in cell metabolism, and its concentration in tissues plays an important role in regulating biochemical reactions , Soleymanlou et al, 2005.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of dextran nanobubbles for oxygen delivery

Cavalli

Bisazza

Giustetto

et al. 2009

International Journal of Pharmaceutics

112

109

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“…Oxygen is one of the key elements in cell metabolism, and its concentration in tissues plays an important role in regulating biochemical reactions , Soleymanlou et al, 2005.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of dextran nanobubbles for oxygen delivery

Cavalli

Bisazza

Giustetto

et al. 2009

International Journal of Pharmaceutics

112

109

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“…Poor placental perfusion appears to result in a cascade of pathological changes: decreased oxygen delivery (6)(7)(8)(9), oxidative stress, formation of reactive oxygen species, endothelial damage (10), increased vascular permeability (11), and inflammation. The role of oxidative stress in PE has been studied by many investigators.…”

Section: Introductionmentioning

confidence: 99%

Placental expression profiling in preeclampsia: local overproduction of hemoglobin may drive pathological changes

Centlow

Carninci²,

Nemeth

et al. 2008

Fertility and Sterility

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Using microarrays made with cDNAs from subtracted placental libraries, we show increased hemoglobin production in the preeclamptic placenta. Heme and hemoglobin may cause endothelial damage and inflammation and drive pathological changes in the placenta if they are released there. Study Objective:To create a library enriched in cDNAs from preeclamptic placentas to print on to microarrays for placental profiling of preeclampsia (PE) and high risk pregnancies. Design: Prospective study.Setting: University women's clinic and academic research laboratory.Patients: Ten patients with PE, 5 with PE and bilateral notching, 5 with bilateral notching without PE, and 15 normotensive patients were recruited.Interventions: Placenta and placenta bed biopsies were collected after delivery. Main Outcome Measures:Subtracted libraries of PE transcripts were produced, and cDNAs from these libraries were used to make PE-specific cDNA arrays. Results were verified quantitatively using real-time PCR and histologically using in situ hybridization and immunohistochemistry.Results: 30 genes were significantly altered in at least one group comparison. Differences in two candidate genes were confirmed using quantitative rt-PCR. Hemoglobin α2 and γ transcripts were significantly over-expressed in the PE placenta. Scattered cells in the placenta and placental blood vessels were shown to express genes encoding these hemoglobin-chains. Conclusions:We demonstrate increased hemoglobin production in the PE placenta. The hemoglobin may be released into the placenta blood vessel lumen. Free heme and hemoglobin are potent toxins which cause endothelial damage and inflammation.

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“…The main source of ROS initiating the pathophysiological events appears to be the placenta [8]. Abnormal vascular development of the blood vessels in the preeclamptic placenta leads to reduced placental perfusion and to induce hypoxia which is by itself a potent stimulus for ROS formation [9]. Also, after initiation of apoptotic pathways, syncytiotrophoblast microvesicles activate maternal neutrophils contributing to oxidative stress and pathophysiology of PE.…”

mentioning

confidence: 99%

Antioxidant Status in Normal Pregnancy and Preeclampsia upon Multivitamin-Mineral Supplementation in the Region of Vojvodina

Ćebović¹,

Marić²,

Nikolić³

et al. 2013

IJBBB

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Abstract-Normal pregnancy is associated with oxidative stress and this is even increased during preeclampsia (PE). The decrease in total antioxidant capacity is the pathophysiological basis for vitamin supplementation during pregnancy, but the beneficial effect of this approach is still unclear. Levels of creatinine, urea, cystatin, malondialdehyde (MDA), superoxide dysmutase activity (SOD), glutathione peroxidase (GSH-Px) activity and acidum ascorbicum were measured in the serums of all women. Mean MDA level in maternal plasma in normal pregnancies was significantly lower than in PE pregnancies (2,78 ±0.78 pmol/mg vs 3.20±0.91 pmol/mg, p< 0.05), as well as mean GSH-Px levels (811 ±206 U/l vs 1350±575 U/l, p< 0.05. Mean ascorbic acid values were significantly higher in normal than in PE pregnancies (9,85 ±2,43 mg/L vs 5.54±1.81 mg/L, p< 0.001), as well as mean SOD values (26.8 ±18.45% vs 12.3±10.3%, p< 0.001). There were no significant differences in normal pregnancy group with and without multivitamin supplementation. MDA levels were significantly lower in PE pregnancies with vitamin supplementation (2.99 ± 0.81 pmol/mg vs 3.42 ± 1.01 pmol/mg, p< 0.05), as well as GSC-Px levels (1200 ± 500 U/l vs 1500 ± 650 U/l, p< 0.05). Ascorbic acid values were significantly higher in the PE group with vitamin supplementation (6.16 ± 1.66 mg/L vs 4.92 ± 1.96 mg/L, p< 0.05), as well as SOD levels (13 ± 11.4 % vs 11.6 ± 9.2%, p< 0.001).

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Molecular Evidence of Placental Hypoxia in Preeclampsia

Abstract: Our results provide molecular evidence that aberrant global placental gene expression changes in preeclampsia may be due to reduced oxygenation and that these events can successfully be mimicked by in vivo and in vitro models of placental hypoxia.

Cited by 336 publications

References 59 publications

Preparation and characterization of dextran nanobubbles for oxygen delivery

Preparation and characterization of dextran nanobubbles for oxygen delivery

Placental expression profiling in preeclampsia: local overproduction of hemoglobin may drive pathological changes

Antioxidant Status in Normal Pregnancy and Preeclampsia upon Multivitamin-Mineral Supplementation in the Region of Vojvodina

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