Molecular evidence from 3′-terminus sequence analysis that tobacco vein-banding mosaic virus is a distinct member of the potyvirus group

Abstract

The 3'-terminal 1902 nucleotides of the tobacco vein-banding mosaic virus (TVBMV) were cloned and sequenced. The sequence contains a large open reading frame of 572 amino acid residues followed by a non-coding region of 183 nucleotides which terminates in a polyadenylate tract. The TVBMV coat protein is processed from a larger polyprotein by cleavage at a Gln/Gly dipeptide, which is followed closely by the aphid transmission DAG triplet. Translation of the nucleotide sequence yielded a protein of 271 amino acid residues with a calculated M(r) of 30,347. The amino acid and nucleotide sequences of the TVBMV coat protein and 3' non-coding region were compared to 33-35 other virus sequences. Percent sequence identity of the amino acid and nucleotide sequences were in the range expected from comparison of distinct viruses. Phylogenetic analyses were also performed to determine the relationship of TVBMV to other well-characterized viruses. These studies indicate that TVBMV should be regarded as a distinct member of the potyvirus group.

Analysis of the sequence diversity of the P1, HC, P3, NIb and CP genomic regions of several yam mosaic potyvirus isolates: implications for the intraspecies molecular diversity of potyviruses.

“…An updated comparison of CP sequences has led to a classification of members of the family Potyviridae into genera, distinct species, related species and strains (Ward & Shukla, 1994 ;Shukla et al, 1994). Other regions of the potyviral genome, such as the 3h untranslated region, have also been used to identify and classify potyviruses (Frenkel et al, 1989 ;Habera et al, 1994).…”

Pest categorisation of non‐EU viruses and viroids of potato

“…Literature: Datura stramonium (Roggero et al, 2000), Nicotiana tabacum (Reddick et al, 1992;Habera et al, 1994), Sesamum indicum (Wang et al, 2017) Experimental hosts in different botanical families (Salazar and Harrison, 1978c …”

The complete genomic sequence of Tobacco vein banding mosaic virus and its similarities with other potyviruses

“…Tobacco vein banding mosaic virus (TVBMV) was first discovered in Taiwan in 1964 [1] and confirmed to be a distinct species of the largest plant virus genus, Potyvirus, in 1994 [2,3]. TVBMV was once a major threat to tobacco production in some regions including Taiwan and northern USA, but only occurred rarely in most parts of China [4][5][6].…”