Molecular epidemioloic analysis of Mycobacterium bovis isolates from Mexico

Milián-Suazo, Feliciano; Salman, M. D.; Black, William C.; Triantis, Joni; Payeur, Janet B.; Torres, Melaine C.

doi:10.2460/ajvr.2000.61.90

Cited by 18 publications

(6 citation statements)

References 34 publications

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“…In contrast, there have been several reports of widespread M. bovis infection in Mexican cattle 18–21,23,32,41. In some regions of Mexico, up to 13% of dairy herds are reported to be infected with M. bovis 42 and up to 30% of milk produced in Mexico is not pasteurized 19.…”

Section: Discussionmentioning

confidence: 98%

Tracing the origins of Mycobacterium bovis tuberculosis in humans in the USA to cattle in Mexico using spoligotyping

Rodwell

Kapasi

Moore

et al. 2010

International Journal of Infectious Diseases

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Objectives To compare genotypes of Mycobacterium bovis strains from humans in Southern California with genotypes of M. bovis strains in cattle in Mexico and the USA to explore the possible origins of human infections. Methods We conducted a descriptive analysis of M. bovis genotypes from a binational population of humans and cattle using spacer oligonucleotide typing (spoligotyping). Results One hundred six human M. bovis spoligotypes were compared to spoligotypes from 496 Mexican cattle and 219 US cattle. Twelve spoligotype patterns were identified among human cases and 126 spoligotype patterns were detected in cattle. Over 91% (97/106) of the human M. bovis isolates had spoligotypes that were identical to those found in Mexican cattle. Four human cases had spoligotypes that matched both cattle born in Mexico and in the USA. Nine human cases had spoligotypes that did not match cattle born in Mexico or the USA. Conclusions Our data indicate that the population of M. bovis strains causing human TB disease in Southern California is closely related to the M. bovis strain population found in Mexican cattle and supports existing epidemiological evidence that human M. bovis disease in San Diego likely originated from Mexican cattle.

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Section: Discussionmentioning

confidence: 98%

Tracing the origins of Mycobacterium bovis tuberculosis in humans in the USA to cattle in Mexico using spoligotyping

Rodwell

Kapasi

Moore

et al. 2010

International Journal of Infectious Diseases

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“…In developed countries, such as Australia, Canada, the USA and New Zealand have either eradicated the disease or very nearly eradicated it, with wild fauna reservoirs preventing total eradication [ 6 ]. In Mexico, however, bTB is endemic, and although 85.8% of the national territory shows a prevalence under 0.5% [ 7 ], high milk production regions have prevalences as high as 16% [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping

et al. 2018

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Mycobacterium bovis infection in cattle persists in Mexico, posing a threat to human health. Control of bovine tuberculosis, through the National Program Against Bovine Tuberculosis, has led to the decrease of disease prevalence in most of the country, except for high dairy production regions. Genotyping of M. bovis has been performed mainly by spoligotyping and variable number tandem repeats (VNTR), but higher resolution power can be useful for a finer definition of the spread of the disease. Whole genome sequencing and spoligotyping was performed for a set of 322 M. bovis isolates from different sources in Mexico: Baja California, Coahuila, Estado de Mexico, Guanajuato, Hidalgo, Jalisco, Queretaro and Veracruz, from dairy and beef cattle, as well as humans. Twelve main genetic clades were obtained through WGS and genetic diversity analysis. A clear differentiation of the Baja California isolates was seen as they clustered together exclusively. However, isolates from the central states showed no specific clustering whatsoever. Although WGS proves to have higher resolving power than spoligotyping, and since there was concordance between WGS and spoligotyping results, we consider that the latter is still an efficient and practical method for monitoring bovine tuberculosis in developing countries, where resources for higher technology are scarce.

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“…Because of its simplicity, RAPD is usually used for genotyping organisms, as a PCR-based fingerprinting method [ 210 ]. It was initially established for the typing of M. tuberculosis isolates, as described previously [ 211 ]. However, it is rarely used for M. bovis because of poor results in terms of discrimination ability [ 207 , 212 ].…”

Section: Partial-genome Typing Of Mtbcmentioning

confidence: 99%

Mycobacterium tuberculosis complex in wildlife: Review of current applications of antemortem and postmortem diagnosis

et al. 2020

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Tuberculosis (TB) is a chronic inflammatory and zoonotic disease caused by Mycobacterium tuberculosis complex (MTBC) members, which affects various domestic animals, wildlife, and humans. Some wild animals serve as reservoir hosts in the transmission and epidemiology of the disease. Therefore, the monitoring and surveillance of both wild and domestic hosts are critical for prevention and control strategies. For TB diagnosis, the single intradermal tuberculin test or the single comparative intradermal tuberculin test, and the gamma-interferon test, which is regarded as an ancillary test, are used. Postmortem examination can identify granulomatous lesions compatible with a diagnosis of TB . In contrast, smears of the lesions can be stained for acid-fast bacilli, and samples of the affected organs can be subjected to histopathological analyses. Culture is the gold standard test for isolating mycobacterial bacilli because it has high sensitivity and specificity compared with other methods. Serology for antibody detection allows the testing of many samples simply, rapidly, and inexpensively, and the protocol can be standardized in different laboratories. Molecular biological analyses are also applicable to trace the epidemiology of the disease. In conclusion, reviewing the various techniques used in MTBC diagnosis can help establish guidelines for researchers when choosing a particular diagnostic method depending on the situation at hand, be it disease outbreaks in wildlife or for epidemiological studies. This is because a good understanding of various diagnostic techniques will aid in monitoring and managing emerging pandemic threats of infectious diseases from wildlife and also preventing the potential spread of zoonotic TB to livestock and humans. This review aimed to provide up-to-date information on different techniques used for diagnosing TB at the interfaces between wildlife, livestock, and humans.

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Molecular epidemioloic analysis of Mycobacterium bovis isolates from Mexico

Abstract: Use of RAPD-PCR could not differentiate M. bovis isolates by epidemiologic characteristics or identify common sources of infection.

Cited by 18 publications

References 34 publications

Tracing the origins of Mycobacterium bovis tuberculosis in humans in the USA to cattle in Mexico using spoligotyping

Tracing the origins of Mycobacterium bovis tuberculosis in humans in the USA to cattle in Mexico using spoligotyping

Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping

Mycobacterium tuberculosis complex in wildlife: Review of current applications of antemortem and postmortem diagnosis

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