Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping

Perea, Claudia; Rodríguez-Hernández, Elba; Ponce, Sergio Iván Román; Suazo, Feliciano Milián; Robbe‐Austerman, Suelee; Stuber, Tod; Alarcón, Germinal Jorge Cantó

doi:10.1371/journal.pone.0201981

Cited by 22 publications

(30 citation statements)

References 57 publications

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“…Since the whole-genome sequence of the first M. bovis strain became available in 2003 (Garnier et al, 2003), increasing efforts have been made to sequence additional strains and use whole-genome information to tackle bovine and/or wildlife TB transmission within specific outbreaks or countries (Bruning-Fann et al, 2017;Sandoval-Azuara et al, 2017;Ghebremariam et al, 2018;Kohl et al, 2018;Lasserre et al, 2018;Orloski et al, 2018;Price-Carter et al, 2018;Razo et al, 2018). However, no studies to date have comprehensively analyzed M. bovis genomes at a global scale to provide insights into its populational structure and evolution based on whole-genome information.…”

Section: Introductionmentioning

confidence: 99%

Global Distribution and Evolution of Mycobacterium bovis Lineages

et al. 2020

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Mycobacterium bovis is the main causative agent of zoonotic tuberculosis in humans and frequently devastates livestock and wildlife worldwide. Previous studies suggested the existence of genetic groups of M. bovis strains based on limited DNA markers (a.k.a. clonal complexes), and the evolution and ecology of this pathogen has been only marginally explored at the global level. We have screened over 2,600 publicly available M. bovis genomes and newly sequenced four wildlife M. bovis strains, gathering 1,969 genomes from 23 countries and at least 24 host species, including humans, to complete a phylogenomic analyses. We propose the existence of four distinct global lineages of M. bovis (Lb1, Lb2, Lb3, and Lb4) underlying the current disease distribution. These lineages are not fully represented by clonal complexes and are dispersed based on geographic location rather than host species. Our data divergence analysis agreed with previous studies reporting independent archeological data of ancient M. bovis (South Siberian infected skeletons at ∼2,000 years before present) and indicates that extant M. bovis originated between 715 and 3,556 years BP, with later emergence in the New World and Oceania, likely influenced by trades among countries.

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Section: Introductionmentioning

confidence: 99%

Global Distribution and Evolution of Mycobacterium bovis Lineages

et al. 2020

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“…Whole-genome sequencing of M. bovis has been used to track transmission within and between cattle and wildlife populations [21,[33][34][35][36][37]. These studies have demonstrated that genomics adds unprecedented resolution, in comparison to previous molecular-typing technologies, in many cases distinguishing infection between individual animals.…”

Section: Introductionmentioning

confidence: 99%

Mycobacterium bovis genomics reveals transmission of infection between cattle and deer in Ireland

Crispell

Cassidy

Kenny³

et al. 2020

Microbial Genomics

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Control of bovine tuberculosis (bTB), caused by Mycobacterium bovis , in the Republic of Ireland costs €84 million each year. Badgers are recognized as being a wildlife source for M. bovis infection of cattle. Deer are thought to act as spillover hosts for infection; however, population density is recognized as an important driver in shifting their epidemiological role, and deer populations across the country have been increasing in density and range. County Wicklow represents one specific area in the Republic of Ireland with a high density of deer that has had consistently high bTB prevalence for over a decade, despite control operations in both cattle and badgers. Our research used whole-genome sequencing of M. bovis sourced from infected cattle, deer and badgers in County Wicklow to evaluate whether the epidemiological role of deer could have shifted from spillover host to source. Our analyses reveal that cattle and deer share highly similar M. bovis strains, suggesting that transmission between these species is occurring in the area. In addition, the high level of diversity observed in the sampled deer population suggests deer may be acting as a source of infection for local cattle populations. These findings have important implications for the control and ultimate eradication of bTB in Ireland.

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“…M. bovis is a highly clonal pathogen 8 , where clonal complexes are defined based on chromosomal deletion, spoligotyping and MIRU-VNTR to define dynamics of disease transmission 9,10 . Recently, Whole-Genome Sequencing (WGS) provided new insights into host-pathogen interactions and the dynamics of disease transmission 11–13 . For example, sequencing the genomes of clinical isolates of M. bovis provided an accurate estimates of strain geographical distribution and evolution 14–18 .…”

Section: Introductionmentioning

confidence: 99%

Genomic Polymorphism Associated with the Emergence of Virulent Isolates of Mycobacterium bovis in the Nile Delta

Abdelaal

Spalink

Amer³

et al. 2019

Sci Rep

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Mycobacterium bovis is responsible for bovine tuberculosis in both animals and humans. Despite being one of the most important global zoonotic disease, data related to the ecology and pathogenicity of bovine tuberculosis is scarce, especially in developing countries. In this report, we examined the dynamics of M. bovis transmission among dairy cattle in the Nile Delta of Egypt. Animals belonging to 27 herds from 7 governorates were tested by the Single Intradermal Comparative Skin Tuberculin (SICST), as a preliminary screen for the presence of bovine tuberculosis. Positive SICST reactors were identified in 3% of the animals spread among 40% of the examined herds. Post-mortem examination of slaughtered reactors confirmed the presence of both pulmonary and/or digestive forms of tuberculosis in > 50% of the examined animals. Targeted and whole-genome analysis of M. bovis isolates indicated the emergences of a predominant spoligotype (SB0268) between 2013–2015, suggesting a recent clonal spread of this isolate within the Nile Delta. Surprisingly, 2 isolates belonged to M. bovis BCG group, which are not allowed for animal vaccination in Egypt, while the rest of isolates belonged to the virulent M. bovis clonal complex European 2 present in Latin America and several European countries. Analysis of strain virulence in the murine model of tuberculosis indicated the emergence of a more virulent strain (MBE4) with a specific genotype. More analysis is needed to understand the molecular basis for successful spread of virulent isolates of bovine tuberculosis among animals and to establish genotype/phenotype association.

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Molecular epidemiology of cattle tuberculosis in Mexico through whole-genome sequencing and spoligotyping

Cited by 22 publications

References 57 publications

Global Distribution and Evolution of Mycobacterium bovis Lineages

Global Distribution and Evolution of Mycobacterium bovis Lineages

Mycobacterium bovis genomics reveals transmission of infection between cattle and deer in Ireland

Genomic Polymorphism Associated with the Emergence of Virulent Isolates of Mycobacterium bovis in the Nile Delta

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