Tracing the origins of Mycobacterium bovis tuberculosis in humans in the USA to cattle in Mexico using spoligotyping

Rodwell, Timothy C.; Kapasi, Anokhi J.; Moore, Monica; Milián-Suazo, Feliciano; Harris, Beth; Guerrero, Luis; Moser, Kathleen; Strathdee, Steffanie A.; Garfein, Richard S.

doi:10.1016/j.ijid.2009.11.037

Cited by 44 publications

(35 citation statements)

References 36 publications

(34 reference statements)

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“…The spoligotype pattern of one of the M. bovis isolates was identified as SB0673, which is one of the most frequent spoligotypes in cattle in Mexico. This spoligotype has been identified previously in other cases of human tuberculosis, in Mexico [26], as well as in the United States in patients of Mexican origin [27,28]. The spoligotype from the second isolate was SB0971, which is the fifth most common spoligotype in Mexico, and it has also been identified in humans from previous studies in Mexico, as well as in the United States in patients of Mexican origin.…”

Section: Resultssupporting

confidence: 66%

Whole Genome Sequencing for Detection of Zoonotic Tuberculosis in Queretaro, Mexico

Perea¹,

Suazo²,

Reyes³

et al. 2017

J Infect Dis Preve Med

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A total of 2,736 samples, sputum, urine, and other fluids, collected from 1,154 tuberculosis suspicious patients in Queretaro, Mexico were included in the study. Acid-fast staining and culture in selective mediums, Stonebrink and Lowenstein-Jensen, were performed in all samples. Genotyping of isolates was performed by spoligotyping and single nucleotide polimorfism (SNP) whole genome sequencing. Mycobacterium bovis spoligotypes and SNP-types obtained were compared to those of cattle found in a database. Twenty-one (1.8%) isolates of Mycobacterium were obtained by culture, all from sputum; two (13%) were identified as M. bovis by spoligotyping, SB0673 and SB0971, which are frequently found in cattle in Mexico. From the isolates´ total, 15 were whole genome sequenced, confirming two as M. bovis. The SNP patterns of the two M. bovis isolates from human were similar to those found in cattle in different parts of Mexico.

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Section: Resultssupporting

confidence: 66%

Whole Genome Sequencing for Detection of Zoonotic Tuberculosis in Queretaro, Mexico

Perea¹,

Suazo²,

Reyes³

et al. 2017

J Infect Dis Preve Med

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“…On the other hand, in recent years, the develop of an alternative technology of molecular classification of the members of the complex M. tuberculosis called spoligotyping has been successfully applied in the investigations of molecular epidemiology of the tuberculosis, with special usefulness in bovine tuberculosis (13)(14)(15)(16).…”

Section: Materiales Y Métodosmentioning

confidence: 99%

“…Por otra parte, en años recientes, se desarrolló una técnica alternativa de tipificación molecular de los miembros del complejo M. tuberculosis denominada spoligotyping que ha sido exitosamente aplicada en las investigaciones de epidemiologia molecular de la tuberculosis, con especial utilidad en tuberculosis bovina (13)(14)(15)(16).…”

Section: Introductionunclassified

First approach to molecular epidemiology of bovine tuberculosis in Colombia

Jojoa-Jojoa

et al. 2016

Rev MVZ Córdoba

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Objective. To investigate the presence of Mycobacterium bovis and other Mycobacterium species in samples of cattle and buffalo in Colombia, to start the molecular characterization of M. bovis in the country. Material and methods. 492 samples were collected from herds identified with the presence of infected animals through the PPD, by the Group of Bovine Tuberculosis ICA Colombian Agricultural Institute in eight departments of Colombia. Lymph nodes of head, thorax and abdomen, gross lesions of tissues with tuberculosis, nasal swabs, milk, blood and fresh cheeses were included. Samples were subjected to detection of Mycobacterium bovis and other mycobacteria by conventional microbiological analysis and PCR-6110 and spoligotyping molecular assays. Results. In the samples analyzed especially in lymph nodes, Mycobacterium bovis was demonstrated with genotypes reported and not previously reported in the world, as well as M. tuberculosis in Antioquia, Cundinamarca, Boyacá and Magdalena departments. Conclusions. In Colombia there are at least 7 genotypes of M. bovis that are infected and sick cattle and buffalo from four different departments becoming serious threat to public health.Key words: Bovine tuberculosis, Mycobacterium bovis, diagnostic, molecular epidemiology, zoonosis (Sources: DeSC, MeSH). RESUMENObjetivo. Investigar la presencia de Mycobacterium bovis y otras especies de Mycobacterium en muestras de ganado bovino y búfalino en Colombia e iniciar la caracterización molecular de M. bovis en el país. Materiales y métodos. 492 muestras fueron recogidas de hatos identificados con presencia de animales infectados a través de la prueba de PPD, por el Grupo de Tuberculosis Bovina del Instituto Colombiano Agropecuario ICA en ocho departamentos de Colombia. Se incluyeron ganglios linfáticos de cabeza, tórax y abdomen, tejidos con lesiones macroscópicas de tuberculosis, hisopos nasales, leche, sangre y quesos frescos. Las muestras se sometieron a investigación de Mycobacterium bovis y otras micobacterias mediante el análisis microbiológico convencional y pruebas moleculares de PCR-IS6110 y spoligotyping. Resultados. En las muestras analizadas especialmente en los ganglios linfáticos, se evidenció Mycobacterium bovis con genotipos reportados y no reportados previamente en el mundo, así como M. tuberculosis en los departamentos de Antioquia, Cundinamarca, Magdalena y Boyacá. Conclusiones. En Colombia existen al menos 7 genotipos de M. bovis que se encuentran infectando y enfermando a bovinos y bufalinos de cuatro diferentes departamentos del país constituyéndose en seria amenaza para la salud pública. INTRODUCCIÓNMycobacterium bovis, agente causal de la tuberculosis en el ganado, es también un patógeno para gran número de animales como los felinos silvestres, animales domésticos y primates humanos y no humanos, entre otros, por lo cual es considerado un agente zoonótico importante (1). Aunque M. tuberculosis es el agente etiológico más frecuente de la tuberculosis humana, ésta también puede ser producida por M...

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“…In addition, PCR amplification of ribosomal sequences (Ribotyping) or amplification of repetitive intragenic consensus sequences (i.e. ERIC-PCR, spoligotyping, MIRU-VNTR), among others, are the usual methods used to specifically discriminate among different M. tuberculosis strains (Rodwell et al, 2010;Pang et al, 2011).…”

Section: Introductionmentioning

confidence: 99%