Molecular epidemiology of Cryptosporidium in livestock animals and humans in the Ismailia province of Egypt

Helmy, Yosra A.; Krücken, Jürgen; Nöckler, Karsten; Samson‐Himmelstjerna, Georg von; Zessin, Karl-Hans

doi:10.1016/j.vetpar.2012.12.015

Cited by 139 publications

(146 citation statements)

References 48 publications

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“…Theoretically, it was known that C. parvum is infectious to many mammalian hosts worldwide (Fayer et al, 2006;Santin and Zarlenga, 2009). Calves were the major recognized reservoirs for C. parvum (Caccio et al, 2000;Warda et al, 2002;Condoleo et al, 2007;Paul et al, 2008;Helmy et al, 2013;Mahfouz et al, 2014) with strong relation between C. parvum infection and diarrhea among Egyptian buffalo calves (Warda et al, 2002). Cryptospordium parvum was mostly dominant in preweaned calves (El-Dessouky and El-Masry, 2005;Santin et al, 2008;Keshavarz et al, 2009;Karanis et al, 2010;Randhawa et al, 2012a).…”

Section: Discussionmentioning

confidence: 99%

“…Cryptosporidium frequently affected and strong relation between C. parvum infection and diarrhea among Egyptian buffalo calves (Warda et al, 2002). Cryptosporidium parvum, C. ryanae and C. bovis were identified as 65.7, 11.8 and 4.1%, respectively, with combinations of C. parvum plus C. ryanae (11.2%), C. parvum plus C. bovis (5.3%) and of C. parvum plus C. andersoni (1.8%) in Egyptian buffaloes from Ismailia province (Helmy et al, 2013). Also, it was found that C. parvum was the dominant species in buffaloes and cattle in Ismailia province (65.7%) (Helmy et al, 2015).…”

Section: Introductionmentioning

confidence: 97%

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Molecular Characterization of Bubaline Isolate of Cryptosporidium Species from Egypt

Abdelrahma¹,

Megeed²,

Hammam³

et al. 2015

Research J. of Parasitology

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Cryptosporidium an apicomplexan parasite has the ability to induce diarrhea in bovines, goats, pigs, dogs and cats worldwide. In this study, buffalo calves fecal samples were examined after staining their smears with Modified Ziehl-Neelsen Stain (MZN). Ileal sections were examined for the detection of pathological changes. Further molecular characterization was done using nested PCR amplification and partial sequence analysis. The detected oocysts were morphologically similar to Cryptosporidium parvum. Light microscopic examination of Cryptosporidium infected ileal Tissue Section (TS) stained with H and E revealed the presence of altered mucosal architecture with congestion of blood vessels, infiltration, sloughing and complete erosion of epithelial cells and shortening, blunting, stunting and atrophy of the intestinal villi. Molecular characterization gave PCR amplicons of 18S SSU rRNA gene products approximately at 823 bp. Sequences proved specified generalized relatedness with 21 species of Cryptosporidium but the nucleotide homogeneity percentage was insufficient to designate species or genotypes. Further bioinformatics analysis showed that resulting Cryptosporidium isolates had the closest match with three isolates. It was implied that the Cryptosporidium isolates is mostly like Cryptosporidium parvum (JX237832.1) previously isolated from buffaloes in Ismailia province.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Molecular Characterization of Bubaline Isolate of Cryptosporidium Species from Egypt

Abdelrahma¹,

Megeed²,

Hammam³

et al. 2015

Research J. of Parasitology

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“…This species has previously been reported in buffalo in Egypt (Helmy et al 2013), South Africa (Abu Samra et al 2013), Australia (Abeywardena et al 2013a), and China . In the previous study in Australia, C. ryanae variants (reported as genotypes 1 and 2) and a C. suis-like genotype (reported as genotype 3) were also reported in buffaloes (Abeywardena et al 2013a).…”

Section: Discussionmentioning

confidence: 62%

“…However, relatively little is known about the range of species and genotypes of Cryptosporidium in other members of the family Bovidae, including water buffalo (Bubalus bubalis), but to date, C. parvum, C. ryanae, C. bovis, C. ubiquitum, a "C. suis-like" genotype, and C. ryanae variants have been reported (Gómez-Couso et al 2005;Cacciò et al 2007;Feng et al 2012;Venu et al 2012;Abeywardena et al 2013a;Abu Samra et al 2013;Amer et al 2013;Helmy et al 2013;Inpankaew et al 2014;Mahfouz et al2014;Ma et al 2015;Abeywardena et al 2015;Aquino et al 2015;Helmy et al 2015).…”

Section: Introductionmentioning

confidence: 99%

First report of Cryptosporidium species in farmed and wild buffalo from the Northern Territory, Australia

Zahedi

Phasey²,

Boland³

et al. 2016

Parasitol Res

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A molecular epidemiological survey of Cryptosporidium from water buffalo (Bubalus bubalis) in the Northern Territory in Australia was conducted. Fecal samples were collected from adult farmed (n = 50) and wild buffalo (n = 50) and screened using an 18S quantitative PCR (qPCR). Positives were typed by sequence analysis of 18S nested PCR products. The qPCR prevalence of Cryptosporidium species in farmed and wild buffalo was 30 and 12 %, respectively. Sequence analysis identified two species: C. parvum and C. bovis, with C. parvum accounting for ~80 % of positives typed from the farmed buffalo fecal samples compared to 50 % for wild buffalo. Subtyping at the 60 kDa glycoprotein (gp60) locus identified C. parvum subtypes IIdA19G1 (n = 4) and IIdA15G1 (n = 1) in the farmed buffalo and IIaA18G3R1 (n = 2) in the wild buffalo. The presence of C. parvum, which commonly infects humans, suggests that water buffaloes may contribute to contamination of rivers and waterways with human infectious Cryptosporidium oocysts, and further research on the epidemiology of Cryptosporidium in buffalo populations in Australia is required.

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“…Dessa forma, a caracterização molecular de isolados de diferentes origens (animal, humana e ambiental) tem sido amplamente usada com o intuito de investigar o potencial zoonótico das espécies ou genótipos do protozoário (XIAO;FAYER, 2008). Atualmente, 26 espécies de Cryptosporidium são reconhecidas, sendo nove capazes de infectar humanos (ASSIS et al, 2013;SHARMA et al, 2013;KATZER, 2013), sendo que diversos grupos de subtipos e sub-genótipos de Cryptosporidium têm sido identificados, dos quais os subtipos Cryptosporidium parvum IIa e IId foram identificados como zoonóticos (PLUTZER; KARANIS, 2009;HELMY et al, 2013).…”

Section: Com O Advento Da Síndrome Da Imunodeficiênciaunclassified

Criptosporidiose em animais domésticos: aspectos epidemiológicos

et al. 2013

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ResumoEstudos referentes á criptosporidiose são essenciais, devido sua relevância em saúde pública e por sua patogenicidade em animais de produção e de companhia. Nos últimos 20 anos, houve uma rápida expansão das pesquisas envolvendo o gênero Cryptosporidium, em grande parte relacionada a estudos moleculares, propiciando a descrição de várias espécies, genótipos e subtipos do parasito. A caracterização molecular de isolados de diferentes origens (animal, humana e ambiental) tem sido amplamente usada com o intuito de investigar o potencial zoonótico deste protozoário. As formas de transmissão documentadas são de animais para o ser humano, de pessoa para pessoa, por meio de ingestão ou do uso de água destinada a lazer contaminada direta ou indiretamente com oocistos esporulados. A elevada taxa de animais naturalmente infectados e a susceptibilidade ao protozoário justificam a importância de se atentar para a ocorrência desta enfermidade. Assim, são demonstrados aspectos epidemiológicos desta zoonose em animais domésticos. Palavras-chave: Cryptosporidium spp., ruminantes, equinos, caninos, felinos AbstractStudies related cryptosporidiosis will be essential, due to its relevance in public health and pathogenicity in pets and production animals. Over the past 20 years, there has been a rapid expansion of research involving the Cryptosporidium genus, largely related to molecular studies, providing a description of

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Molecular epidemiology of Cryptosporidium in livestock animals and humans in the Ismailia province of Egypt

Cited by 139 publications

References 48 publications

Molecular Characterization of Bubaline Isolate of Cryptosporidium Species from Egypt

Molecular Characterization of Bubaline Isolate of Cryptosporidium Species from Egypt

First report of Cryptosporidium species in farmed and wild buffalo from the Northern Territory, Australia

Criptosporidiose em animais domésticos: aspectos epidemiológicos

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