Molecular Characterization of Bubaline Isolate of Cryptosporidium Species from Egypt

Abstract

Cryptosporidium an apicomplexan parasite has the ability to induce diarrhea in bovines, goats, pigs, dogs and cats worldwide. In this study, buffalo calves fecal samples were examined after staining their smears with Modified Ziehl-Neelsen Stain (MZN). Ileal sections were examined for the detection of pathological changes. Further molecular characterization was done using nested PCR amplification and partial sequence analysis. The detected oocysts were morphologically similar to Cryptosporidium parvum. Light microscopic examination of Cryptosporidium infected ileal Tissue Section (TS) stained with H and E revealed the presence of altered mucosal architecture with congestion of blood vessels, infiltration, sloughing and complete erosion of epithelial cells and shortening, blunting, stunting and atrophy of the intestinal villi. Molecular characterization gave PCR amplicons of 18S SSU rRNA gene products approximately at 823 bp. Sequences proved specified generalized relatedness with 21 species of Cryptosporidium but the nucleotide homogeneity percentage was insufficient to designate species or genotypes. Further bioinformatics analysis showed that resulting Cryptosporidium isolates had the closest match with three isolates. It was implied that the Cryptosporidium isolates is mostly like Cryptosporidium parvum (JX237832.1) previously isolated from buffaloes in Ismailia province.

Anticryptosporidial effect of pomegranate peels water extract in experimentally infected mice with special reference to some biochemical parameters and antioxidant activity

“…This agreed with previous findings that the interval which covered the natural Cryptosporidium oocyst shedding period in mice was about 24 days (Matsui et al 2001) or about 3-4 weeks (Lacroix et al 2001). Also, in this study, C. parvum oocysts detected in mice fecal smears were morphologically similar to those of C. parvum described in previous studies (Hassanain et al 2011;Abdelrahman et al 2015). In this study, a gradual reduction in oocyst shedding was noticed in all infected animal groups.…”

“…Light microscopic examination of C. parvum-infected ileal sections revealed the presence of altered mucosal architecture, with sloughing and complete erosion of epithelial cells, shortening, blunting, stunting and atrophy of the intestinal villi. These findings agreed with Abu El Ezz et al 2011and Abdelrahman et al (2015). Sasahara et al (2003) found that at the time when the number of C. parvum oocysts in the ileum was maximal, absorptive and columnar goblet cells decreased in the ileal epithelium with a significant reduction in the height of the villi.…”

Therapeutic Efficacy of Ginger (Zingiber officinale), Ginseng (Panax ginseng) and Sage (Salvia officinalis) Against Cryptosporidium parvum in Experimentally Infected Mice

“…2 (2020) THERAPEUTIC EFFICACY OF GINGER (Zingiberofficinale), GINSENG (Panax ginseng) … of histopathological corrections. These results agreed with Abu El Ezz et al [12], Abdelrahman et al [43] and Abouel-Nour et al [44]. In this study, the intestinal villi of infected ginger, ginseng high dose treated mice groups showed more histologic repair and normal tissue appearance in comparison to control and other treated groups.…”

Anticryptosporidium Efficacy of Olea europaea and Ficus carica Leaves Extract in Immunocompromised Mice Associated with Biochemical Characters and Antioxidative System

“…This result should be attributed to the immunodeficient situation that flared up the infection. This was evident research [46], and another study [47] found a higher number of developmental stages in the epithelial cells of the infected control group's gut [47,48]. A reduction in the villus to crypt ratio in mice infected with C. parvum oocysts was observed by some studies.…”