Molecular epidemiology and drug resistance of Acinetobacter baumannii isolated from a regional hospital in the Brazilian Amazon region

Ribeiro, Edlainny Araujo; Gales, Ana Cristina; Oliveira, Ana Paula Streling de; Coelho, Danilo Dias; Oliveira, Rodrigo Alves de; Pfrimer, Irmtraut Araci Hoffmann; Filho, José Rodrigues do Carmo

doi:10.1590/0037-8682-0087-2020

Cited by 7 publications

(5 citation statements)

References 9 publications

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“…In keeping with this, this pathogen's role in seafood samples is potentially unknown, and further research is required to understand this fact. High prevalence of resistance of A. baumannii strains toward commonly-used antimicrobials, particularly tetracycline, gentamicin, ciprofloxacin, trimethoprim, streptomycin, and erythromycin was reported from Brazil [41], Iran [42], United States [43], Jordan [44], Africa [45], and Korea [46].As antibiotic-resistant A. baumannii was detected in large number of seafood samples. Proper monitoring of the seafood microbial quality should perform at the site of fishing and also after their transport.…”

Section: Discussionmentioning

confidence: 99%

The First Report of the Antimicrobial Resistance of Acinetobacter baumannii Isolates from Seafood Samples

et al. 2021

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A cinetobacterbaumannii is a newly-launched bacterium responsible for rare cases of diarrhea and foodborne diseases. This survey was performed to study the prevalence and antimicrobial resistance of A. baumannii isolates recovered from fish, shrimp, and lobster. Five-hundred seafood samples were collected and cultured for isolation of A. baumannii. A. baumannii isolates were identified by biochemical tests and further confirmed by the PCR. The antimicrobial resistance of isolates was studied using disk diffusion test. Twenty-eight out of 500 (5.6%) seafood samples were contaminated with A. baumannii. Fish had the highest prevalence of A. baumannii (10%), while lobster had the lowest (2.5%). A. baumannii isolates harbored the highest incidence of resistance toward tetracycline (75%), gentamicin (71.4%), ciprofloxacin (64.2%), trimethoprim (57.1%), streptomycin (53.5%), and erythromycin (50%) antimicrobial agents. The lowest incidence of resistance was obtained against chloramphenicol (3.5%) and imipenem (7.1%) antimicrobial agents. This is the first report of isolation and identification and antimicrobial resistance pattern of A. baumannii in seafood samples globally. The role of seafood samples as reservoirs of antimicrobial-resistant A. baumannii was determined. Complete cooking of seafood and prevention from their consumption in raw form can diminish the risk of A. baumannii foodborne diseases.

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Section: Discussionmentioning

confidence: 99%

The First Report of the Antimicrobial Resistance of Acinetobacter baumannii Isolates from Seafood Samples

et al. 2021

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“…A. baumannii is a critical pathogen of nosocomial infections and can easily cause serious illnesses, such as ventilator-related pneumonia, sepsis, surgical wounds, and urinary tract infections. A. baumannii can adapt to the environment and acquire exogenous drug-resistance genes [ 31 , 32 ]. It shows a high degree of natural inherent drug resistance and acquired resistance to commonly used antibacterial drugs of various chemical structures [ 33 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

Synergistic Role of Biofilm-Associated Genes and Efflux Pump Genes in Tigecycline Resistance of Acinetobacter baumannii

Luo

Wang

et al. 2023

Med Sci Monit

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Background Previous research reported that the resistance mechanism of Acinetobacter baumannii resistance to tigecycline was mainly related to the overexpression of the AdeABC efflux pump system. Biofilm formation is a notable pathogenesis of A. baumannii infections and antibiotic resistance. Our study explores the latent relevance of biofilm-associated genes and efflux pump genes in A. baumannii tigecycline resistance. Material/Methods A total of 78 clinical samples were collected from October 2018 to October 2019. Seventy-two clinically isolated A. baumannii strains were divided into a tigecycline-resistant Acinetobacter baumannii (TR-AN) group and tigecycline-sensitive Acinetobacter baumannii (TS-AN) group by tigecycline minimum inhibitory concentration tests. The biofilm formation of the 2 groups was observed using crystal violet staining. Furthermore, biofilm-related genes and efflux pump genes were analyzed by RT-PCR. Results The biofilm-forming rate of the TR-AN group was 82.2%, and that of the TS-AN group was 14.8%. The biofilm synthesis gene bfs was 91.3% positive in the TR-AN group, significantly higher than in the TS-AN group at the transcription level ( P <0.05). The minimum inhibitory concentration of tigecycline was higher in the TR-AN group with biofilm formation than in the TR-AN group without biofilm formation ( P <0.05). The efflux pump AdeB gene was 95.2% positive in the TR-AN group with biofilm formation and 38.7% positive in the TR-AN group without biofilm formation. Conclusions The biofilm formation of A. baumannii may be positively related to tigecycline resistance ability because of the co-expression of the bfs gene and the AdeB efflux pump gene. The enhanced transcription level of bfs and AdeB promotes biofilm formation to improve the resistance of A. baumannii to tigecycline.

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“…18 Além disso, no hospital em estudo, já foi possível evidenciar que 55,6% dos A. baumannii isolados carreavam o gene blaOXA-23, associado à resistência aos carbapenêmicos, uma das últimas opções terapêuticas em infecções graves. 19 Portanto, o perfil fenotípico de multirresistência descrito nesta pesquisa sugere a necessidade de medidas mais severas para controle de infecção, uso racional de antimicrobianos e vigilância contínua. 19 Apesar das recomendações robustas acerca das boas práticas assistenciais, uma avaliação em unidades ambulatoriais de hemodiálise, constatou que a indicação e duração para a profilaxia não são consistentes com as diretrizes em 20% dos casos, o que pode piorar o panorama associado a resistência bacteriana e o prognóstico dos pacientes.…”

Section: Dessaunclassified

“…19 Portanto, o perfil fenotípico de multirresistência descrito nesta pesquisa sugere a necessidade de medidas mais severas para controle de infecção, uso racional de antimicrobianos e vigilância contínua. 19 Apesar das recomendações robustas acerca das boas práticas assistenciais, uma avaliação em unidades ambulatoriais de hemodiálise, constatou que a indicação e duração para a profilaxia não são consistentes com as diretrizes em 20% dos casos, o que pode piorar o panorama associado a resistência bacteriana e o prognóstico dos pacientes. [20][21] Além disso, é preciso considerar que as instalações de diálise ambulatorial são ambientes propícios para a disseminação de organismos multirresistentes, reforçando a necessidade da identificação e investigação de cepas semelhantes entre os pacientes e o ambiente, evidenciando a ocorrência de transmissão cruzada.…”

Section: Dessaunclassified

Occurrence and resistance profile of bacteria belonging to the ESKAPE group in hemodialysis patients

Ribeiro

Alves²,

Alves³

2023

Rev. Pre Infec e Saúde

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Introduction: Hemodialysis is the main treatment for patients with Chronic Renal Insufficiency and one of the main complications resulting from it is the occurrence of infections caused by opportunistic microorganisms belonging to the “ESKAPE” group. Aim: to determine the frequency of isolation of pathogens from the ESKAPE group and evaluate their respective antimicrobial resistance profiles in hemodialysis patients in a medium and high complexity hospital located in the Amazon. Outlining: Retrospective study carried out with collection of data available in the medical records of 167 patients diagnosed with Chronic Renal Insufficiency, undergoing hemodialysis or peritoneal dialysis from 2015 to 2019 in a regional hospital in the Southeast of Pará. Results: Of the 174 bacteriological cultures analyzed, 70.1% were positive and, of these, 57.4% were positive for bacteria belonging to the ESKAPE group. The most frequent microorganism belonging to the ESKAPE group was Staphylococcus aureus (47.1%); as to the resistance levels, 50% of Acinetobacter baumannii were resistant to carbapenems. Implications: The phenotypic profile of multidrug resistance to antimicrobials in strains of Acinetobacter baumannii was identified, highlighting the importance of knowledge about the profile of sensitivity to antimicrobials for a more assertive treatment and better prognosis.

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Molecular epidemiology and drug resistance of Acinetobacter baumannii isolated from a regional hospital in the Brazilian Amazon region

Cited by 7 publications

References 9 publications

The First Report of the Antimicrobial Resistance of Acinetobacter baumannii Isolates from Seafood Samples

The First Report of the Antimicrobial Resistance of Acinetobacter baumannii Isolates from Seafood Samples

Synergistic Role of Biofilm-Associated Genes and Efflux Pump Genes in Tigecycline Resistance of Acinetobacter baumannii

Occurrence and resistance profile of bacteria belonging to the ESKAPE group in hemodialysis patients

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