Molecular detection of tungro virus on mekongga and inpari 4 rice varieties in West Papua

Hamdayanty, H Hamdayanty H; Ladja, Fausiah T.; Mansur, Mansur

doi:10.1088/1755-1315/807/2/022088

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…It has caused a severe reduction in rice production in several countries, including Indonesia. Tungro has spread in 34 provinces in Indonesia [1] and has been identified as a disease caused by two different virus infections, namely Rice Tungo Bacilliform Virus (RTBV) and Rice tungro Spherical Virus (RTSV) [2]- [6]. They are spread semipersistent by the green leafhopper, Nephotettix virescens (Distant), and certain other leafhopper species IOP Publishing doi:10.1088/1755-1315/1160/1/012054 2 [5].…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of rice tungro bacilliform virus (RTBV) on weed host in South Sulawesi

Rosida

Senoaji

Ibrahim

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Rice tungro bacilliform Virus (RTBV) is one of the viruses that cause tungro disease in rice plants causing leaf yellow and plant stunting. Some weed species have also been described as potential hosts for RTBV in addition to rice. However, RTBV cannot be diagnosed in weeds only by looking at their morphological symptoms because weeds infected with RTBV do not show typical symptoms. Hence this research was conducted to detect the presence of RTBV molecularly on several weed hosts in rice fields in South Sulawesi. The polymerase Chain Reaction technique with coat protein gene-specific primers was used to detect RTBV. A total of 29 leaf samples consisting of 12 weed species collected from Sidrap, Pinrang, Maros, and Gowa were then extracted genomic DNA from the leaf samples for PCR analysis. The results of PCR analysis showed that weed samples that tested positive for RTBV were Panicum repens (Lawawoi/Sidrap), Echinochloa crusgalli (Lawawoi/Sidrap), Cyperus difformis (Lanrang/Sidrap), Echinochloa crusgalli (Palangga/Gowa), Fimbristilys miliacea (Lanrang/Sidrap), and Eleusine indica (Lawawoi/Sidrap) which were indicated by the presence of RTBV virus DNA fragments with 430 bp in length in all these weeds. Weeds infected by RTBV formed 2 clusters. The first cluster consisted of RTBV in P.repens and E.crusgalli from Lawawoi/Sidrap, RTBV in C.difformis and F.miliacea from Lanrang/Sidrap, and RTBV in E.indica from Palangga/Gowa. At the same time, the second cluster consisted of RTBV in E.indica from Lawawoi/Sidrap.

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Section: Introductionmentioning

confidence: 99%

Molecular detection of rice tungro bacilliform virus (RTBV) on weed host in South Sulawesi

Rosida

Senoaji

Ibrahim

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Coat Protein 1 Gene of Indonesian Isolates of Rice Tungro Spherical Virus showed High Divergence with those from other South and South East Asian Regions

Praptana,

Santosa,

Rahmini

et al. 2023

TOASJ

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Background: Tungro, an important viral disease in rice, is a co-infection of rice tungro bacilliform virus (RTBV) and rice tungro spherical virus (RTSV). Severe symptoms were reported in rice fields on two major islands in Indonesia: Jawa (Magelang District) and Sulawesi (Sidrap District). Objective: Sequences of global isolates currently registered in NCBI GenBank were examined with two new Indonesian isolates to understand the phylogeny of RTSV based on different genome regions as well as to spot the gap in the molecular data of Indonesian isolates. Methods: Transmission test of RTSV using the vector green leaf hopper was performed, and then the complete sequences of coat protein 1 (CP1) gene of Magelang and Sindrap districts were molecularly studied using MEGA X software and Recombination Detection Program. Results: The obtained complete sequence of coat protein 1 (CP1) gene of Magelang-7 (OQ426495) and Sindrap-9 (OQ426496) isolates were aligned with 39 isolates in NCBI GenBank. Sequences of other isolates in GenBank were also retrieved and then aligned according to six individual Open Reading Frames (ORFs) and ‘Full ORF’. Recombination analysis detected significant signals in the genomes of one partially and four fully sequenced isolates. Phylogenetic study also confirmed the frequent recombination among RTSV isolates clustered in two geographically separated groups: South East Asia and South Asia. Conclusion: Although the available data is still rudimentary, this study demonstrated that the CP1 gene of 21 isolates from different regions of Indonesia was highly identical among themself but divergent with isolates from other countries. Therefore, deep molecular comprehension on Indonesian isolates is necessary to manage the recurrent Tungro spread in the country.

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Molecular detection of tungro virus on mekongga and inpari 4 rice varieties in West Papua

Cited by 2 publications

References 12 publications

Molecular detection of rice tungro bacilliform virus (RTBV) on weed host in South Sulawesi

Molecular detection of rice tungro bacilliform virus (RTBV) on weed host in South Sulawesi

Coat Protein 1 Gene of Indonesian Isolates of Rice Tungro Spherical Virus showed High Divergence with those from other South and South East Asian Regions

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