Molecular detection of in-vivo microbial contamination of metallic orthodontic brackets by checkerboard DNA-DNA hybridization

Andrucioli, Marcela Cristina Damião; Filho, Paulo Nelson; Matsumoto, Mírian Aiko Nakane; Saraiva, Maria da Conceição Pereira; Feres, Magda; Figueiredo, Luciene Cristina; Martins, Renato Parsekian

doi:10.1016/j.ajodo.2011.06.036

Cited by 40 publications

(42 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The dynamic alterations in the oral microbial equilibrium caused by the orthodontic appliances [10,12] could be modulated by the bracket design [31]. In the present study, the incidence percentages showed different patterns for the three different brackets, confirming this fact.…”

Section: Discussionsupporting

confidence: 86%

“…However, they could not establish any clinical significance with this pattern of colonization. Andruciloli et al [10] observed that the metallic brackets were multi-colonized, with the species of the purple, green, orange, and red complexes found in the early stages of orthodontic treatment.…”

Section: Introductionmentioning

confidence: 98%

“…Orthodontic appliances have frequently shown interference in the homeostatic balance of the oral cavity [10,11]. However, few studies have evaluated the colonization of the microbial species harboring in the different bracket types.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Microbial complexes levels in conventional and self-ligating brackets

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Microbial complexes levels in conventional and self-ligating brackets

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…Looking at previous studies, Purmal et al 1 found M luteus, S haemolyticus, and A calcoaceticus, 1 whereas Hong et al 5 found an increase in Streptococcus mutans in the saliva and biofilm after the placement or orthodontic brackets. 5 With regard to the pathogenicity of microorganisms, Andrucioli et al 14 had already underscored that high levels of oral microorganisms increase the risk not only of caries and periodontal disease but also of systematic complications. 13 According to Levinson and Jawetz 15 S epidermidis may cause endocarditis, a rare but possible complication of dental treatment that will only develop in the presence of bacteremia.…”

Section: Discussionmentioning

confidence: 99%

In vitro evaluation of microbial contamination of orthodontic brackets as received from the manufacturer using microbiological and molecular tests

Gerzson

Simon

Anjos

et al. 2015

The Angle Orthodontist

View full text Add to dashboard Cite

Objective: To test the null hypothesis that orthodontic brackets as supplied by manufacturers do not have microbial contamination. Materials and Methods: The sample comprised 140 brackets of four different commercially available brands, used directly from the manufacturer's packaging, divided into 14 groups (n 5 10 brackets each). Of the 140 pieces, 60 were full cases and 80 were replacement brackets. Materials were tested to detect bacterial growth, analyze types of bacteria present (biochemical test), and identify bacteria (molecular test with polymerase chain reaction [PCR]). Results: In two of 12 groups the brackets showed microbial contamination: group 1, Morelli full case brackets, and group 12, Abzil-3M Unitek replacement brackets. Staphylococcus aureus and Staphylococcus epidermidis were the bacteria identified in groups 1 and 12, respectively (suggested by the biochemical test and confirmed by PCR). Conclusions: Brackets of two brands (Morelli and Abzil-3M Unitek) were found to be contaminated by bacteria in the original packages supplied by the manufacturers, which suggests a risk for patient contamination. These data suggest that the manufacturers of these materials should improve the quality control of the packaging used, including sterilization, for the security of patient health. (Angle Orthod. 2015;85:992-996.)

show abstract

“…Orthodontic appliances are made from a variety of solid and elastic materials with irregular surfaces and these irregularities increase the retention of food residue, making mechanical hygiene difficult (1,2), increasing microbial contamination (3)(4)(5)(6). Orthodontic appliances can change oral microflora, increase the levels of periodontal pathogens (7) and Streptococcus mutans (1,(8)(9)(10) in the saliva and dental biofilms during orthodontic treatment.…”

Section: Introductionmentioning

confidence: 99%

Quantification of Streptococcus mutans in Different Types of Ligature Wires and Elastomeric Chains

et al. 2017

Self Cite

View full text Add to dashboard Cite

This study aimed to test the hypothesis that Streptococcus mutans contamination levels differ according to the type of the orthodontic ligature. Thirteen patients were selected. Each quadrant was randomly subjected to one of the following ligature-use protocols: I) elastomeric chain, II) steel ligature crossed over the archwire, III) steel ligature crossed under the archwire, and IV) steel ligature in a figure-eight pattern under the archwire. After seven days, the devices were removed and the Streptococcus mutans colony-forming unit count per mg of biofilm weight was determined. Twelve specimens (n=3) were also processed for scanning electron microscopy analysis. ANOVA and Tukey-Kramer test were used for comparisons to assess S. mutans differences between groups at a 5% significance level. There was no statistical difference in detectable levels of S. mutans among the groups (p=0.294). Scanning electron microscopy results showed abundant biofilms and microbial contamination in all groups. In conclusion, S. mutans contamination levels are similar in the different orthodontic ligatures.

show abstract

Molecular detection of in-vivo microbial contamination of metallic orthodontic brackets by checkerboard DNA-DNA hybridization

Cited by 40 publications

References 26 publications

Microbial complexes levels in conventional and self-ligating brackets

Microbial complexes levels in conventional and self-ligating brackets

In vitro evaluation of microbial contamination of orthodontic brackets as received from the manufacturer using microbiological and molecular tests

Quantification of Streptococcus mutans in Different Types of Ligature Wires and Elastomeric Chains

Contact Info

Product

Resources

About