Molecular Detection of blaOXA-51 in Carbapenem-resistant Acinetobacterbaumannii Isolated from Different Clinical Sources

doi:10.37506/mlu.v20i4.1900

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…The suspension of the test organism was prepared by picking parts of similar test colonies with a sterile wire loop, suspended in sterile normal saline, and incubated for 2 h to allow organisms to reach their log phase in growth. The densities of suspension or turbidity of bacteria to inoculate were determined by comparison with the standard on McFarland 0.5 Barium sulfate solution (19).…”

Section: Sampling Process and Laboratory Analysismentioning

confidence: 99%

Indoor air bacterial load and antibiotic susceptibility pattern of isolates at Adare General Hospital in Hawassa, Ethiopia

Atalay,

Mengistie,

Tolcha

et al. 2023

Front. Public Health

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BackgroundAir is the agent of pathogenic microbes that cause significant problems in the hospital environment. Multidrug resistance poses a major therapeutic challenge to these airborne microorganisms in hospital indoor environments.Method and materialsThis study was conducted at Adare General Hospital in Hawassa City, Sidama, Ethiopia. A cross-sectional study was conducted. The proportional allocation method was used to select the sampled 50 rooms from the total available rooms in each category of wards and staff offices. A total of 100 indoor air samples were collected using settle plates in all units twice a day, morning (9:00–4:00 a.m.) and afternoon (3:00–4:00 p.m.). The types and number of colonies were determined in the laboratory, and the pathogenic bacteria were isolated by appropriate bacteriological techniques. Antimicrobial susceptibility testing was performed on Mueller-Hinton agar for each potentially pathogenic bacterium isolated. For each bacterium, a total of 12 antibiotics were tested using the Kirby-Bauer disk diffusion method. The test organism was adjusted to McFarland turbidity standards of 0.5. Data were entered and analyzed using the SPSS version 25 window. Descriptive analysis and one-way analysis of variance were performed.ResultsThe indoor air bacterial load of Adare General Hospital was found in the range between 210 and 3,224 CFU/m3. The highest indoor air bacterial load was identified from the gynecology ward with a mean of 2,542.5CFU/m3 at p < 0.05. From 100 indoor air samples, a total of 116 bacterial pathogen isolates were obtained. Gram-positive isolates predominated at 72.4%, of which 37.1% were Staphylococcus aureus, 26.7% were coagulase-negative Staphylococci, and the rest 8.6% were Streptococcus pyogenes. The isolation of pathogenic bacteria Staphylococcus aureus and coagulase-negative Staphylococci showed a high level of resistance to ampicillin.ConclusionA high bacterial load was found in the study area as compared to different indoor air biological standards. Staphylococcus aureus and coagulase-negative Staphylococci were the isolated predominant bacteria. Attention should be given to preventing and minimizing those environmental factors that favor the multiplication of bacteria in the indoor environment of a hospital for the safe health of patients, visitors, and staff.

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Section: Sampling Process and Laboratory Analysismentioning

confidence: 99%

Indoor air bacterial load and antibiotic susceptibility pattern of isolates at Adare General Hospital in Hawassa, Ethiopia

Atalay,

Mengistie,

Tolcha

et al. 2023

Front. Public Health

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Study the Epidemiology of Antibiotics Resistance in Ramadi Hospitals of Iraq

Najeeb

Meani

Abdulkareem

et al. 2022

RJPT

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In many hospitals around the world, antibiotic resistance has reached a crisis. Mainly, Staphylococcus aureus (MRSA), which is immune to methicillin, is swamped and many with Gram-negative multidrug-resistant (MDR). The occurrence of carbapenmases for the first time was considered a major problem because of several factors, such as: many plasmids carrying gene resistance elements take on other resistance elements, including QnrA (QnrA and QnrB) and aminoglycoside (rmtB) Resistance elements. For severe MRSA infections, good treatment options are available, death rates continue to be high. The condition is more complicated and disturbing for MDR Gram-negatives. In developing countries, there are few new agents who can profit from the situation in the coming decade . Although the meanings are muddled, some patients in intensive care are thought to die because of the lack of antibiotics effective against Pseudomonas aeruginosa and A. baumanni. If molecular resistance is to be overcome, additional understanding is needed urgently. We additionally need to install early warning systems to keep pace with new resistances.

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Assessment of Cinamic acid and Costus roots extract against MDR-K. pneumoniae isolated from patients of COVID-19

Hussein

Al-Qaysi

Meani

et al. 2022

RJPT

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The emergence of resistant Klebsiella pneumoniae associated with COVID-19 demonstrate a primary challenge for the antimicrobial therapy of infectious diseases and increases the incidence of mortality and morbidity. "K. pneumoniae isolated from COVID-19 patient's sputum with ratio (100%). All K. pneumoniae clinical isolates had 100% resistance to ceftriaxone, piperacillin (80%), cefepime (60%), amikacin (40%), and meropenem =levofloxccin (20%). Bacterial isolates gave positive result for mCIM with ratio was 100%, also all isolates produced diversity of ‎ β- lactamases at a rate of 100% by using spectrometry β-lactamase assay. Costunolide (38.3 %), Rutin (15.33%), Pentadecanoic acid (6.54%), Oliec acid (4.77%), and Caproic acid (3.22%) considered as major compounds in Saussurea costus were identified by GC-Mass spectrometry. The β-lactamase produced by K. pneumoniae were inhibited by Saussurea costus with a strong statistical significance at P- value: <0.01, while cinamic acid donot effected on β-lactamase activity.

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Molecular Detection of blaOXA-51 in Carbapenem-resistant Acinetobacterbaumannii Isolated from Different Clinical Sources

Cited by 3 publications

References 8 publications

Indoor air bacterial load and antibiotic susceptibility pattern of isolates at Adare General Hospital in Hawassa, Ethiopia

Indoor air bacterial load and antibiotic susceptibility pattern of isolates at Adare General Hospital in Hawassa, Ethiopia

Study the Epidemiology of Antibiotics Resistance in Ramadi Hospitals of Iraq

Assessment of Cinamic acid and Costus roots extract against MDR-K. pneumoniae isolated from patients of COVID-19

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