Assessment of Cinamic acid and Costus roots extract against MDR-K. pneumoniae isolated from patients of COVID-19

Abstract

The emergence of resistant Klebsiella pneumoniae associated with COVID-19 demonstrate a primary challenge for the antimicrobial therapy of infectious diseases and increases the incidence of mortality and morbidity. "K. pneumoniae isolated from COVID-19 patient's sputum with ratio (100%). All K. pneumoniae clinical isolates had 100% resistance to ceftriaxone, piperacillin (80%), cefepime (60%), amikacin (40%), and meropenem =levofloxccin (20%). Bacterial isolates gave positive result for mCIM with ratio was 100%, also all isolates produced diversity of ‎ β- lactamases at a rate of 100% by using spectrometry β-lactamase assay. Costunolide (38.3 %), Rutin (15.33%), Pentadecanoic acid (6.54%), Oliec acid (4.77%), and Caproic acid (3.22%) considered as major compounds in Saussurea costus were identified by GC-Mass spectrometry. The β-lactamase produced by K. pneumoniae were inhibited by Saussurea costus with a strong statistical significance at P- value: <0.01, while cinamic acid donot effected on β-lactamase activity.

Antibacterial and antibiofilm activity of cinnamic acid against Multidrug-resistant bacteria isolated from Ramadi Teaching hospitals, Iraq

“…Consequently, cinnamic acid shows promise as a safe treatment option for skin wound infections (Mingoia et al, 2022) . A new search study has shown that cinnamic acid effectively hindered the formation of biofilms in K. pneumoniae (Hussein et al, 2022).One suggested mechanism suggests that phenolic compounds may target the peptidoglycan within the bacterial cell wall, thereby influencing their ability to inhibit biofilm formation (Bali et al, 2019). Furthermore, another suggested mechanism potentially inhibits N-acyl homoserine lactones (AHLs)mediated quorum sensing (Zhang et al 2020).…”

“…Only recently, the significance of biofilm has been developed as a crucial microbial survival strategy, along with its substantial impact on various human activities, been recognized and understood (Claessen et al 2014). The five stages of biofilm formation include i) Attachment, ii) Colonization, iii) Proliferation, iv) Maturation and v) Dispersion (Hussein et al 2022). In light of the increasing antibiotic resistance, there is a pressing need to search for alternative inhibitory drugs.…”

Impact of Solidago virgaurea Extract on Biofilm Formation for ESBL-Pseudomonas aeruginosa: An In Vitro Model Study

“…Pharmaceuticals 2023, 16, x FOR PEER REVIEW 3 and economic feasibility [8,9]. S. virgaurea (SV) consisted of many active compounds lead for using it in the treatment of many diseases, including kidney inflammation, nary tract infection, cystitis, diarrhea and arthritis [10,11].…”

“…These sources are particularly appealing due to their minimal toxicity, beneficial pharmacological effects Due to advancements in scientific knowledge regarding the medicinal attributes of plants, there has been a surge of interest in natural sources of antibiotics. These sources are particularly appealing due to their minimal toxicity, beneficial pharmacological effects and economic feasibility [8,9]. S. virgaurea (SV) consisted of many active compounds that lead for using it in the treatment of many diseases, including kidney inflammation, urinary tract infection, cystitis, diarrhea and arthritis [10,11].…”