Molecular Design of β-Hairpin Peptides for Material Construction

Rughani, Ronak V.; Schneider, Joel P.

doi:10.1557/mrs2008.106

Cited by 64 publications

(59 citation statements)

References 47 publications

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“…Specifically, these hydrogels were found to exhibit minimal-to-no cytotoxicity to a variety of cell lines including NIH3T3 murine fibroblasts, C3H10T1/2 mesenchymal stem cells, hepatocytes, MG63 osteoblast progenitor cells, and primary articular chondrocytes [410,411,466,467]. The homogeneous distribution C3H10T1/2 cells in MAX8 and MAX1 hydrogels is dependent on the gelation kinetics.…”

Section: Shear Stress Responsive Hydrogelsmentioning

confidence: 99%

Stimulus-responsive hydrogels: Theory, modern advances, and applications

Koetting

Peters

Steichen

et al. 2015

Materials Science and Engineering: R: Reports

893

688

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Over the past century, hydrogels have emerged as effective materials for an immense variety of applications. The unique network structure of hydrogels enables very high levels of hydrophilicity and biocompatibility, while at the same time exhibiting the soft physical properties associated with living tissue, making them ideal biomaterials. Stimulus-responsive hydrogels have been especially impactful, allowing for unprecedented levels of control over material properties in response to external cues. This enhanced control has enabled groundbreaking advances in healthcare, allowing for more effective treatment of a vast array of diseases and improved approaches for tissue engineering and wound healing. In this extensive review, we identify and discuss the multitude of response modalities that have been developed, including temperature, pH, chemical, light, electro, and shear-sensitive hydrogels. We discuss the theoretical analysis of hydrogel properties and the mechanisms used to create these responses, highlighting both the pioneering and most recent work in all of these fields. Finally, we review the many current and proposed applications of these hydrogels in medicine and industry.

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Section: Shear Stress Responsive Hydrogelsmentioning

confidence: 99%

Stimulus-responsive hydrogels: Theory, modern advances, and applications

Koetting

Peters

Steichen

et al. 2015

Materials Science and Engineering: R: Reports

893

688

View full text Add to dashboard Cite

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“…4A). This significant increase in solubility is largely due to the eight lysines contained within the SVS-1 sequence, which at physiologic pH are protonated [23]. CD spectroscopy confirmed that conjugation of PTX to PEG-GG-SVS-1 did not prevent folding of the peptide at the surface of phosphatidylserine containing liposomes (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Cancer cell surface induced peptide folding allows intracellular translocation of drug

Medina

2015

Journal of Controlled Release

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Many lead molecules identified in drug discovery campaigns are eliminated from consideration due to poor solubility and low cell permeability. These orphaned molecules could have clinical value if solubilized and delivered properly. SVS-1 is a de novo designed peptide that preferentially folds at the surface of tumor cells, adopting a β-hairpin conformation that rapidly translocates into the cytoplasm, and ultimately nucleus, of cells. SVS-1 is stable in serum and small molecules attached to the peptide are effectively delivered to cancer cells via mechanisms involving physical translocation and, to a lesser extent, clathrin-dependent endocytosis. For example, ligating the model hydrophobic drug Paclitaxel (PTX) to SVS-1 improved its aqueous solubility by ~1000-fold and successfully delivered and released PTX to cancer cells in vitro and tumors in vivo without toxic adjuvants. These results suggest SVS-1 can serve as a simple, effective delivery platform for molecules with poor solubility and permeability.

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“…These domains can be spatially separated either along the length of the molecule or along a defined face of the folded molecule. 39 The hydrophilic segments can either be uncharged (polar) or charged (cationic, anionic or zwitterionic) 40 . While such segments are spatially segregated in lipids, in amphipathic peptides the primary sequences contain alternating or random segments of hydrophobic and hydrophilic residues.…”

Section: Structures Of Self-assembling Nanoparticlesmentioning

confidence: 99%

“…103 Glycol-, peptide- and silicon-capped dendrimers have been synthesized using carbohydrates, peptides and silicon. 39,105 Drugs can associate with dendrimers through physical entrapment within the void spaces or by attachment of the pro-drug to functional groups positioned on the dendrimer surface. 94 …”

Section: Structures Of Self-assembling Nanoparticlesmentioning

confidence: 99%

A review of solute encapsulating nanoparticles used as delivery systems with emphasis on branched amphipathic peptide capsules

Barros

Whitaker

Sukthankar

et al. 2016

Archives of Biochemistry and Biophysics

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Various strategies are being developed to improve delivery and increase the biological half-lives of pharmacological agents. To address these issues, drug delivery technologies rely on different nano-sized molecules including: lipid vesicles, viral capsids and nano-particles. Peptides are a constituent of many of these nanomaterials and overcome some limitations associated with lipid-based or viral delivery systems, such as tune-ability, stability, specificity, inflammation, and antigenicity. This review focuses on the evolution of bio-based drug delivery nanomaterials that self-assemble forming vesicles/capsules. While lipid vesicles are preeminent among the structures; peptide-based constructs are emerging, in particular peptide bilayer delimited capsules. The novel biomaterial— Branched Amphiphilic Peptide Capsules (BAPCs) display many desirable properties. These nano-spheres are comprised of two branched peptides— bis(FLIVI)-K-KKKK and bis(FLIVIGSII)-K-KKKK, designed to mimic diacyl-phosphoglycerides in molecular architecture. They undergo supramolecular self-assembly and form solvent-filled, bilayer delineated capsules with sizes ranging from 20 nm to 2 microns depending on annealing temperatures and time. They are able to encapsulate different fluorescent dyes, therapeutic drugs, radionuclides and even small proteins. While sharing many properties with lipid vesicles, the BAPCs are much more robust. They have been analyzed for stability, size, cellular uptake and localization, intra-cellular retention and, bio-distribution both in culture and in vivo.

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Molecular Design of β-Hairpin Peptides for Material Construction

Cited by 64 publications

References 47 publications

Stimulus-responsive hydrogels: Theory, modern advances, and applications

Stimulus-responsive hydrogels: Theory, modern advances, and applications

Cancer cell surface induced peptide folding allows intracellular translocation of drug

A review of solute encapsulating nanoparticles used as delivery systems with emphasis on branched amphipathic peptide capsules

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