Molecular control of the female germline stem cell niche size in Drosophila

Hsu, Hwei-Jan; Bahader, Majid; Lai, Chun-Ming

doi:10.1007/s00018-019-03223-0

Cited by 12 publications

(14 citation statements)

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“…In D. melanogaster, the formation and differentiation of ovarian follicles is triggered by 20E (see Ameku et al, 2017;Belles and Piulachs, 2015;Hsu et al, 2019;König et al, 2011;Uryu et al, 2015). The triggering effect of 20E on somatic and germinal cells in the germarium, appear ancestral and also operating in less modified insects, where juvenile hormone plays a gonadotropic role (Belles et al, 2000;Comas et al, 2001;Treiblmayr et al, 2006).The source of ecdysone in adult B. germanica females is the ovary, and at this age, their main function is to promote chorion synthesis in mature basal ovarian follicles (Pascual et al, 1992;Romaña et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

“…In fact, the latter may be mediated by the Notch pathway. Ecdysone signalling induces Dl expression at the terminal filament in cell membranes, which activates N and determines the fate of these cells, which can become cap or escort cells (Ameku et al, 2017;Green et al, 2011;Hsu et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…In D. melanogaster, the formation and differentiation of ovarian follicles are triggered by 20E signalling (see Ameku et al, 2017;Belles and Piulachs, 2015;Hsu et al, 2019;König et al, 2011;Uryu et al, 2015). Therefore, we presumed that this mechanism might also operate in B. germanica.…”

Section: Ecdysone Signalling and The Differentiation Of Ovarian Follimentioning

confidence: 99%

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Eyes absent in the cockroach panoistic ovaries regulates proliferation and differentiation through ecdysone signalling

Ramos

Chelemen

Pagone

et al. 2020

Insect Biochemistry and Molecular Biology

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Eyes absent in the cockroach panoistic ovaries regulates proliferation and differentiation through ecdysone signalling

Ramos

Chelemen

Pagone

et al. 2020

Insect Biochemistry and Molecular Biology

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“…In D. melanogaster , the formation and differentiation of ovarian follicles are controlled by 20E (see Belles and Piulachs, 2015; Hsu et al, 2019; König et al, 2011; Uryu et al, 2015). This type of regulation may be ancestral and could also operate in insects where juvenile hormone plays the role of gonadotropic hormone (Bellés et al, 2000; Comas et al, 2001; Treiblmayr et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…In fact, this latter may be mediated by Notch pathway. Ecdysone signalling induces Dl expression at the terminal filament in cell membranes, which activates N and determines the fate of these cells, which can become cap or escort cells (Ameku et al, 2017; Green et al, 2011; Hsu et al, 2019). Our results in B. germanica suggest that similar signalling networks occur in panoistic ovaries.…”

Section: Discussionmentioning

confidence: 99%

eyes absentin the cockroach panoistic ovaries regulates proliferation and differentiation through ecdysone signalling

Ramos

Chelemen

Pagone

et al. 2019

Preprint

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21Eye absent (Eya) is a protein which has been structurally conserved from hydrozoans to 22 humans which has two functions: it is both a transcription cofactor and a protein 23 tyrosine phosphatase. Eya was first described in the fly Drosophila melanogaster for its 24 role in eye development, and the same functions were also later reported in less derived 25 insects. Studies on the involvement of Eya in insect oogenesis are limited to D. 26 melanogaster, which has meroistic ovaries. In this fly, Eya plays a fundamental role in 27 the first stages of ovarian development because Eya mutations abolish gonad formation. 28 In this present work we studied the function of Eya in the panoistic ovary of the 29 cockroach Blattella germanica. We demonstrated that Eya is essential for correct ovary 30 development also in this ovary type. In B. germanica ovaries, Eya affects both somatic 31 and germinal cells in the germarium and the vitellarium, acting differently in different 32 ovarian regions. Development of the basal ovarian follicles is arrested BgEya-depleted 33 females, while in the germaria, BgEya helps to the maintain the correct number of 34 somatic and germinal stem cells by regulating the expression of ecdysteroidogenic 35 genes in the ovary.36 37 38 39 40 41 42 43 44 45 46 KEYWORDS: panoistic ovary, ecdysone, Halloween genes, cell proliferation, insect 47 oogenesis, Notch 48 49 50 51Maintaining the stability of stem cells is crucial in every organism, and this is especially 52 important in the case of germ stem cells. Oogenesis describes the process of ovary 53 development from the time of germ stem cell differentiation until oocyte maturation. 54During oogenesis, oocytes must synthesize and accumulate all maternal factors needed 55 by embryos to complete their development, thus ensuring reproductive success. 56In insect ovaries, germ stem cells are located in niches in the germarium of each 57 ovariole. The control of their proliferation and differentiation has been thoroughly 58 studied, mainly in species with meroistic polytrophic ovaries such as the fruit fly 59 Drosophila melanogaster (see Ameku et al., 2017; Belles and Piulachs, 2015; Dai et al., 60 2017). In contrast, the number of genes involved in regulating oogenesis so far 61 identified in less modified panoistic ovaries such as those of the cockroaches including 62 Blattella germanica is very limited. This prevents study of the mechanisms that control 63 oocyte growth and maturation in these basal insects. B. germanica is emerging as a 64 choice model in which to study panoistic ovaries. In this cockroach, each ovary has 65 around 20 ovarioles and only the most basal ovarian follicle of each ovariole matures 66 during a given gonadotrophic cycle, a process that starts early in the last nymphal instar. 67The basal ovarian follicles are almost ready to mature in freshly ecdysed females, 68 whereas the development of the remaining ovarian follicles of each ovariole is arrested 69 until these basal ones are oviposited. 70In previous co...

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The mini player with diverse functions: extracellular vesicles in cell biology, disease, and therapeutics

Thakur

Chen

et al. 2021

Protein &Amp; Cell

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Extracellular vesicles (EVs) are tiny biological nanovesicles ranging from approximately 30–1000 nm in diameter that are released into the extracellular matrix of most cell types and in biofluids. The classification of EVs includes exosomes, microvesicles, and apoptotic bodies, dependent on various factors such as size, markers, and biogenesis pathways. The transition of EV relevance from that of being assumed as a trash bag to be a key player in critical physiological and pathological conditions has been revolutionary in many ways. EVs have been recently revealed to play a crucial role in stem cell biology and cancer progression via intercellular communication, contributing to organ development and the progression of cancer. This review focuses on the significant research progress made so far in the role of the crosstalk between EVs and stem cells and their niche, and cellular communication among different germ layers in developmental biology. In addition, it discusses the role of EVs in cancer progression and their application as therapeutic agents or drug delivery vehicles. All such discoveries have been facilitated by tremendous technological advancements in EV-associated research, especially the microfluidics systems. Their pros and cons in the context of characterization of EVs are also extensively discussed in this review. This review also deliberates the role of EVs in normal cell processes and disease conditions, and their application as a diagnostic and therapeutic tool. Finally, we propose future perspectives for EV-related research in stem cell and cancer biology.

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Molecular control of the female germline stem cell niche size in Drosophila

Cited by 12 publications

References 73 publications

Eyes absent in the cockroach panoistic ovaries regulates proliferation and differentiation through ecdysone signalling

Eyes absent in the cockroach panoistic ovaries regulates proliferation and differentiation through ecdysone signalling

eyes absentin the cockroach panoistic ovaries regulates proliferation and differentiation through ecdysone signalling

The mini player with diverse functions: extracellular vesicles in cell biology, disease, and therapeutics

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