Eyes absent in the cockroach panoistic ovaries regulates proliferation and differentiation through ecdysone signalling

Ramos, Saray; Chelemen, Fleur; Pagone, Viviana; Elshaer, Nashwa; Irles, Paula; Piulachs, María‐Dolors

doi:10.1016/j.ibmb.2020.103407

Cited by 10 publications

(9 citation statements)

References 41 publications

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“…However, a high variation could be observed at these time points, suggesting that transcription of these genes may be tightly regulated, showing dynamic pulses of transcript levels when induced. A similar pattern was seen for the transcript levels of BlageShd in the ovaries of the German cockroach B. germanica [ 54 ]. In the brown planthopper, N. lugens , it was suggested that both Nillu CYP307B1 (Spo) and Nillu CYP314A (Shd) are rate-limiting enzymes for ecdysteroidogenesis [ 53 ].…”

Section: Discussionsupporting

confidence: 73%

“…Interestingly, in 2018, Yatsenko and Shcherbata identified a coordinated role between ecdysone signalling and Notch signaling in germline niche formation in Drosophila [ 61 ]. Furtermore, Ramos et al (2020) suggested that the Notch downstream component Eyes Absent affects the ecdysteroidogenic pathway in B. germanica [ 54 ]. Therefore, it is possible that a depletion of Halloween gene expression in S. gregaria might also functionally interact with the Notch signaling pathway and, consequently, disturb the oogenesis process.…”

Section: Discussionmentioning

confidence: 99%

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Knockdown of the Halloween Genes spook, shadow and shade Influences Oocyte Development, Egg Shape, Oviposition and Hatching in the Desert Locust

Schellens

Lenaerts

Baca

et al. 2022

IJMS

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Ecdysteroids are widely investigated for their role during the molting cascade in insects; however, they are also involved in the development of the female reproductive system. Ecdysteroids are synthesized from cholesterol, which is further converted via a series of enzymatic steps into the main molting hormone, 20-hydoxyecdysone. Most of these biosynthetic conversion steps involve the activity of cytochrome P450 (CYP) hydroxylases, which are encoded by the Halloween genes. Three of these genes, spook (spo), phantom (phm) and shade (shd), were previously characterized in the desert locust, Schistocerca gregaria. Based on recent sequencing data, we have now identified the sequences of disembodied (dib) and shadow (sad), for which we also analyzed spatiotemporal expression profiles using qRT-PCR. Furthermore, we investigated the possible role(s) of five different Halloween genes in the oogenesis process by means of RNA interference mediated knockdown experiments. Our results showed that depleting the expression of SchgrSpo, SchgrSad and SchgrShd had a significant impact on oocyte development, oviposition and hatching of the eggs. Moreover, the shape of the growing oocytes, as well as the deposited eggs, was very drastically altered by the experimental treatments. Consequently, it can be proposed that these three enzymes play an important role in oogenesis.

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Section: Discussionsupporting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Knockdown of the Halloween Genes spook, shadow and shade Influences Oocyte Development, Egg Shape, Oviposition and Hatching in the Desert Locust

Schellens

Lenaerts

Baca

et al. 2022

IJMS

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“…The increased expression of phm and shd in 3-day-old females, together with the absence of changes in EcR, and the tendency to increase E75A expression, may explain, at least in part, the significant increase in number of ovarian follicles observed in the ovarioles of dsftz-f1 treated females ( p = 0.03) (Figure 3f-h). This suggests that 20E production was increased in these females, since the number of ovarian follicles in the vitellaria of B. germanica is positively correlated with 20E levels (Ramos et al, 2020). Note: A dose of 1 μg of dsftz-f1 or dsPolyH was injected into the abdomen of newly ecdysed females.…”

Section: Dsftz-f1 Treatment In Early Adults Modifies the Expression O...mentioning

confidence: 99%

“…The follicular cells surrounding the oocytes mature and change its characteristics accompanying the oocyte growth (Figure 1b). It is in the sixth (last) nymphal instar, when the number of ovarian follicles in the ovarioles is established (Ramos et al, 2020), and basal ovarian follicles (BOFs) start to grow. During all the instar the follicular cells proliferate (Figure 1c), and the mitotic activity is maintained until the beginning of the vitellogenic period in adults (3-day-old females) (Irles & Piulachs, 2014).…”

Section: Introductionmentioning

confidence: 99%

Dual function of the transcription factor Ftz‐f1 on oviposition in the cockroach Blattella germanica

Alborzi

Piulachs

2023

Insect Molecular Biology

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The transcription factor Ftz‐f1 has multiple functions in insect development in a spatial‐temporal line. One of these roles is in the insect ovaries, specifically in the regulation of steroidogenic enzymes production. We studied the function of F in Blattella germanica oogenesis, as it shows two moments of high expression in ovaries: before the imaginal moult, and just before ovulation in the adult. Injecting dsftz‐f1 into adult females, either just after the imaginal moult or just prior to choriogenesis, prevented oviposition, with differences between the two approaches. In 3‐day‐old adult females treated with dsftz‐f1 just after the emergence, the expression of ftz‐f1 was not modified, but the steroidogenic genes increased their expression. ftz‐f1 transcript levels in the ovaries of 5‐day‐old dsftz‐f1‐treated females were significantly depleted, and the expression levels of the same steroidogenic genes began to decrease. These results suggest that Ftz‐f1 regulates the expression of steroidogenic genes in B. germanica, with phm possibly being a key target. Ftz‐f1 has a different temporal function in the cytoskeleton of follicular cells of the basal ovarian follicles. Early in the gonadotrophic cycle, Ftz‐f1 promotes the expression of genes related to the cytoskeleton and muscle proteins, while at the end of the cycle it maintains the expression levels of these genes, thus ensuring correct ovulation.

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“…O desenvolvimento dos insetos depende da ação hormonal, especialmente HJ (mencionado acima) e 20-hidroxi-ecdisona (20E) (para revisão, ver Texada et al, 2020). Em particular, os hormônios regulam o desenvolvimento de ovários (Leimar et al, 2012;Hartfelder et al, 2018;Ramos et al, 2020), vias de sinalização, como a Hippo (Mirth e Shingleton, 2012;Dong et al, 2015;Gotoh et al, 2015;Wang et al, 2016), bem como pequenos RNAs não-codificadores, denominados microRNAs (miRNAs) (Belles, 2017).…”

Section: Apis Melliferaunclassified

Expressão de genes da via Hippo em Apis mellifera: relação com hormônios, microRNAs e comparação entre ovários de rainhas e operárias em desenvolvimento

Sanches¹

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Dedico esse trabalho aos meus pais que sempre me apoiaram e estiveram presentes em todas as minhas decisões. AGRADECIMENTOSAgradeço ao Programa de Pós-Graduação em Genética e à Faculdade de Medicina de Ribeirão Preto (FMRP-USP), pela oportunidade de realização deste trabalho e por todo suporte que tive durante o Mestrado; às agências de fomento CAPES, Fapesp e CNPq por todo suporte financeiro; Ao meu Orientador Prof. Dr. Francis Nunes pela oportunidade de desenvolver esse projeto, por todos os ensinamentos, pela amizade e por estar sempre disponível para me auxiliar no desenvolvimento do mesmo; Às professoras, Profa Dra. Zilá Simões, Profa Dra. Márcia Bitondi e ao Prof. Dr. Klaus Hartfelder por disponibilizarem os recursos e o laboratório, e por estarem sempre disponíveis para discutir sobre o projeto; À Profa Dra. Lívia Moda, ao Prof. Dr. Rodrigo Dalaqua, e a MSc. Franciane Rabiço por todo o suporte que me deram a respeito da hibridização in situ; À MSc. Natália Hernandes por ter gentilmente me cedido as amostras de Apis mellifera tratadas com hormônio juvenil e 20-hidroxiecdisona; Aos meus colegas de laboratório e aos alunos do Prof. Dr. Klaus Hartfelder pela boa convivência e pela cooperação que sempre tivemos; Ao técnico Luís Aguiar por tudo que aprendi sobre manutenção do apiário e pelo auxílio nas coletas de abelhas operárias e produção de rainhas da espécie Apis mellifera; À Dra. Denyse Lago por ter se dedicado a me ensinar dissecção de ovários de larvas de Apis mellifera, junto com a Profa. Dra. Zilá; Aos técnicos Vera Figueiredo, Rogério Pereira, por estarem sempre disposto a me auxiliar, pela amizade e todos os momentos felizes no laboratório. Ao técnico Anderson por ter me recebido com tanta gentileza; À especialista do LMMC -EMRP, Elizabete Milani por ter me recebido com tanta gentileza. A dedicação e o amor que ela tem pelo trabalho e pelo projeto das pessoas que a procura é notável e inspirador; Às funcionárias Maria e Francisca pela excelente companhia durante o almoço e no final do expediente, e por manter o laboratório sempre limpo e organizado; Agradeço aos meus pais, Marlene e Luiz, ao meu noivo Pedro, a minha avó Zélia e ao meu irmão Carlos por todo o apoio que me deram durante essa trajetória: por todos os finais de semanas e feriados que me acompanharam nas coletas, pela imensa compreensão que tiveram comigo nas diferentes fases do projeto e por todo o amor que recebo de cada um, eu amo vocês. À Maria Luiza e à Josélia por todo o apoio psicológico durante o período da Pandemia em que estive desenvolvendo o Mestrado.

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Eyes absent in the cockroach panoistic ovaries regulates proliferation and differentiation through ecdysone signalling

Cited by 10 publications

References 41 publications

Knockdown of the Halloween Genes spook, shadow and shade Influences Oocyte Development, Egg Shape, Oviposition and Hatching in the Desert Locust

Knockdown of the Halloween Genes spook, shadow and shade Influences Oocyte Development, Egg Shape, Oviposition and Hatching in the Desert Locust

Dual function of the transcription factor Ftz‐f1 on oviposition in the cockroach Blattella germanica

Expressão de genes da via Hippo em Apis mellifera: relação com hormônios, microRNAs e comparação entre ovários de rainhas e operárias em desenvolvimento

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