Molecular cloning of two novel stearoyl-acyl desaturase genes from winterness wheat

Abstract

Using RT-PCR and rapid amplification of cDNA ends, two new full-length cDNAs of SAD (TaSAD1 and TaSAD2) were obtained from a hardiest winter wheat cultivar (Mironovskaya808). Sequence comparison analysis showed that the deduced amino acid sequences of TaSAD1 and TaSAD2 had high similarity to those of other reported SAD proteins. They were also different each other by some substitutions, insertions and/or deletions involving single amino acid residues or motifs. Based on evolution analysis, it was clear that all SAD genes from Poaceae were closer than those from other genus such as Arabidopsis, Glycine, Triadica, Brassica, Sesamum and Bassia. All SAD genes clustered into two major groups in Poaceae. Meanwhile, TaSAD1 and TaSAD2 were clustered into different groups. The tertiary structure prediction indicated that both TaSAD1 and TaSAD2 proteins were a compact globular protein and their model structures almost were the same.

Transcriptional profiling by DDRT-PCR analysis reveals gene expression during seed development in Carya cathayensis Sarg.

“…As a result, the content of unsaturated fatty acids rose gradually up in the process of seed development. Guo et al (2011) obtained two new full-length cDNAs of stearoyl-acyl desaturase (TaSAD1 and TaSAD2) from a hardiest winter wheat cultivar using RT-PCR and rapid amplification of cDNA ends. Based on evolution analysis, it was clear that all SAD genes from Poaceae were closer than those from other genus such as Arabidopsis, Glycine, Triadica, Brassica, Sesamum and Bassia.…”