Characterization of a stearoyl-acyl carrier protein desaturase gene from potential biofuel plant, Pongamia pinnata L.

Ramesh, Aiyagari; Kesari, Vigya; Rangan, Latha

doi:10.1016/j.gene.2014.03.047

Cited by 13 publications

(9 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar results have been described for the SAD genes from oil palm, 28 Jatropha, 29 C. longepaniculatum, 30 and Pongamia pinnata. 33 In the case of OeSAD1, expression levels remained low in all studied tissues.…”

Section: Tissue Specificity Of Olive Stearoyl-acp Desaturase Genesmentioning

confidence: 86%

Transcriptional Analysis of Stearoyl-Acyl Carrier Protein Desaturase Genes from Olive (Olea europaea) in Relation to the Oleic Acid Content of the Virgin Olive Oil

Parvini

Sicardo

Hosseini‐Mazinani

et al. 2016

J. Agric. Food Chem.

View full text Add to dashboard Cite

The specific contribution of different stearoyl-ACP desaturase (SAD) genes to the oleic acid content in olive (Olea europaea) fruit has been studied. Towards that end, we isolated three distinct cDNA clones encoding three SAD isoforms from olive (cv. Picual), as revealed by sequence analysis. The expression levels of olive SAD genes were determined in different tissues from Picual and Arbequina cultivars, including developing mesocarp and seed, together with the unsaturated fatty acid content. Lipid and gene expression analysis indicate that OeSAD2 seems to be the main gene contributing to the oleic acid content of the olive fruit and, therefore, of the virgin olive oil. This conclusion was confirmed when the study was extended to Hojiblanca, Picudo and Manzanilla cultivars. Furthermore, our data indicate that the olive microsomal oleate desaturase gene OeFAD2-2, but not OeSAD2, is responsible for the linoleic acid content in the virgin olive oil.

show abstract

Section: Tissue Specificity Of Olive Stearoyl-acp Desaturase Genesmentioning

confidence: 86%

Transcriptional Analysis of Stearoyl-Acyl Carrier Protein Desaturase Genes from Olive (Olea europaea) in Relation to the Oleic Acid Content of the Virgin Olive Oil

Parvini

Sicardo

Hosseini‐Mazinani

et al. 2016

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…A recent study has identified four circadian clock genes ( ELF4 , LCL1 , PRR7 , and TOC1 ) of Pongamia and found their expression to be diurnally regulated under long-day conditions [ 20 ]. Two other studies have successively isolated the full-length cDNA clones for two Pongamia desaturase genes ( PpSAD and PpFAD2 ), which have displayed distinct expression patterns during different stages of seed development [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Temporal transcriptome profiling of developing seeds reveals a concerted gene regulation in relation to oil accumulation in Pongamia (Millettia pinnata)

et al. 2018

View full text Add to dashboard Cite

BackgroundPongamia (Millettia pinnata syn. Pongamia pinnata), an oilseed legume species, is emerging as potential feedstock for sustainable biodiesel production. Breeding Pongamia for favorable traits in commercial application will rely on a comprehensive understanding of molecular mechanism regulating oil accumulation during its seed development. To date, only limited genomic or transcript sequences are available for Pongamia, while a temporal transcriptome profiling of developing seeds is still lacking in this species.ResultsIn this work, we conducted a time-series analysis of morphological and physiological characters, oil contents and compositions, as well as global gene expression profiles in developing Pongamia seeds. Firstly, three major developmental phases were characterized based on the combined evidences from embryonic shape, seed weight, seed moisture content, and seed color. Then, the gene expression levels at these three phases were quantified by RNA-Seq analyses with three biological replicates from each phase. Nearly 94% of unigenes were expressed at all three phases, whereas only less than 2% of unigenes were exclusively expressed at one of these phases. A total of 8881 differentially expressed genes (DEGs) were identified between phases. Furthermore, the qRT-PCR analyses for 10 DEGs involved in lipid metabolism demonstrated a good reliability of our RNA-Seq data in temporal gene expression profiling. We observed a dramatic increase in seed oil content from the embryogenesis phase to the early seed-filling phase, followed by a steady and moderate increase towards the maximum at the desiccation phase. We proposed that a highly active expression of most genes related to fatty acid (FA) and triacylglycerol (TAG) biosynthesis at the embryogenesis phase might trigger both the substantial oil accumulation and the membrane lipid synthesis for rapid cell proliferation at this phase, while a concerted reactivation of TAG synthesis-related genes at the desiccation phase might further promote storage lipid synthesis to achieve the maximum content of seed oils.ConclusionsThis study not only built a bridge between gene expression profiles and oil accumulation in developing seeds, but also laid a foundation for future attempts on genetic engineering of Pongamia varieties to acquire higher oil yield or improved oil properties for biofuel applications.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1356-8) contains supplementary material, which is available to authorized users.

show abstract

“…Recipe 9, intended explicitly to be used for children, only contains karanja oil, presented as a natural filter. This oil, obtained from Pongamia pinnata seeds, 13 is rich in unsaturated fatty acids, particularly linoleic and (especially) oleic acids. Due to its lack Although absolutely no reference to any scientific work supporting this claim is provided, looking at this table, we see that it is clearly considered feasible to obtain a concentration of 30% zinc oxide.…”

Section: Discussionmentioning

confidence: 99%

“…Recipe 9, intended explicitly to be used for children, only contains karanja oil, presented as a natural filter. This oil, obtained from Pongamia pinnata seeds, 13 is rich in unsaturated fatty acids, particularly linoleic and (especially) oleic acids. Due to its lack of filtering properties, in a notice to manufacturers dated February 9, 2017, the ANSM (the French National Agency for Medicines and Health Products Safety) indicated that "sunscreen products" containing only karanja oil in order to obtain a protection factor do not comply with current regulations and must not be made available on the market 14 .…”

Section: Discussionmentioning

confidence: 99%

Demonstration of the dangerous nature of ‘homemade’ sunscreen recipes

Couteau

Dupont

Paparis

et al. 2020

J of Cosmetic Dermatology

View full text Add to dashboard Cite

Background: Following a growing mistrust in a certain number of ingredients used by the cosmetic industry, it has become popular in recent years for consumers to make their own products at home. This trend now touches on all areas of the industry, but is especially found in the hygiene sector, with shampoos and toothpaste, and the care sector, with moisturizers and sunscreen, products. Objectives:The objective of this study is to analyze sunscreen recipes found on the Internet and to assess their level of photoprotective efficacy.Materials and Methods: Fifteen Internet recipes were chosen, and then, the products were made in the laboratory following the protocols described. Using an in vitro method, the following efficacy indicators were determined for the preparations made: the Sun Protection Factor (SPF), the Production Factor in the UVA domain (PF-UVA), and the critical wavelength (λ c ).Results: Three of the 15 recipes studied do not contain any sunscreen and therefore constitute a major risk for users in case of exposure to the sun. The other 12 cannot be considered as sun protection products since all of them have an SPF under 6, the threshold value required in Europe. Conclusion:The recipes that we collected on the Internet are very dangerous since some of them have no photoprotective effect whatsoever and most of them do not ensure a sufficient level of photoprotection for the persons using them.

show abstract

Characterization of a stearoyl-acyl carrier protein desaturase gene from potential biofuel plant, Pongamia pinnata L.

Cited by 13 publications

References 55 publications

Transcriptional Analysis of Stearoyl-Acyl Carrier Protein Desaturase Genes from Olive (Olea europaea) in Relation to the Oleic Acid Content of the Virgin Olive Oil

Transcriptional Analysis of Stearoyl-Acyl Carrier Protein Desaturase Genes from Olive (Olea europaea) in Relation to the Oleic Acid Content of the Virgin Olive Oil

Temporal transcriptome profiling of developing seeds reveals a concerted gene regulation in relation to oil accumulation in Pongamia (Millettia pinnata)

Demonstration of the dangerous nature of ‘homemade’ sunscreen recipes

Contact Info

Product

Resources

About