Cloning and Expression of Stearoyl-ACP Desaturase and Two Oleate Desaturases Genes from Ginkgo biloba L.

Wang, Huanli; Cao, Fuliang; Zhang, Wangxiang; Wang, Guibin; Yu, Wanwen

doi:10.1007/s11105-012-0525-4

Cited by 17 publications

(15 citation statements)

References 62 publications

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“…Real-time assays were performed with SYBR Green Dye (Takara, Dalian, China) using a Bio-Rad CFX96 real-time PCR platform (BioRad Laboratories, Hercules, CA, USA) with the following cycle conditions: 95°C for 5 min, followed by 45 cycles of 95°C for 10 s, 60°C for 10 s, and 72°C for 20 s. Three biological replicates were used for each gene. RNA transcript fold changes were calculated using the 2 −Δ ΔCt method (Livak and Schmittgen, 2001 ) with GAPDH as the internal control (Wang et al, 2012 ).…”

Section: Methodsmentioning

confidence: 99%

Transcriptome Profile Analysis from Different Sex Types of Ginkgo biloba L.

Sang

Liu

et al. 2016

Front. Plant Sci.

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In plants, sex determination is a comprehensive process of correlated events, which involves genes that are differentially and/or specifically expressed in distinct developmental phases. Exploring gene expression profiles from different sex types will contribute to fully understanding sex determination in plants. In this study, we conducted RNA-sequencing of female and male buds (FB and MB) as well as ovulate strobilus and staminate strobilus (OS and SS) of Ginkgo biloba to gain insights into the genes potentially related to sex determination in this species. Approximately 60 Gb of clean reads were obtained from eight cDNA libraries. De novo assembly of the clean reads generated 108,307 unigenes with an average length of 796 bp. Among these unigenes, 51,953 (47.97%) had at least one significant match with a gene sequence in the public databases searched. A total of 4709 and 9802 differentially expressed genes (DEGs) were identified in MB vs. FB and SS vs. OS, respectively. Genes involved in plant hormone signal and transduction as well as those encoding DNA methyltransferase were found to be differentially expressed between different sex types. Their potential roles in sex determination of G. biloba were discussed. Pistil-related genes were expressed in male buds while anther-specific genes were identified in female buds, suggesting that dioecism in G. biloba was resulted from the selective arrest of reproductive primordia. High correlation of expression level was found between the RNA-Seq and quantitative real-time PCR results. The transcriptome resources that we generated allowed us to characterize gene expression profiles and examine differential expression profiles, which provided foundations for identifying functional genes associated with sex determination in G. biloba.

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Section: Methodsmentioning

confidence: 99%

Transcriptome Profile Analysis from Different Sex Types of Ginkgo biloba L.

Sang

Liu

et al. 2016

Front. Plant Sci.

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“…Briefly, the 25 μl PCR reaction contained no more than 100 ng cDNA, 12.5 μl SYBR Premix DimerEraser (2×) and 0.3 μM each primer. The reactions were mixed and incubated at 95°C for 30 s, followed by 40 cycles of 95°C for 5 s, 55°C for 30 s and 72°C for 30 s. The expression levels were normalized to the internal control GAPDH [ 77 , 78 ]. The relative expression levels were also analyzed using the 2 -ΔΔCT method.…”

Section: Methodsmentioning

confidence: 99%

Identification and Characterization of MicroRNAs in Ginkgo biloba var. epiphylla Mak

Zhang

Sang

et al. 2015

PLoS ONE

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Ginkgo biloba, a dioecious plant known as a living fossil, is an ancient gymnosperm that stands distinct from other gymnosperms and angiosperms. Ginkgo biloba var. epiphylla (G. biloba var. epiphylla), with ovules borne on the leaf blade, is an unusual germplasm derived from G. biloba. MicroRNAs (miRNAs) are post-transcriptional gene regulators that play critical roles in diverse biological and metabolic processes. Currently, little is known about the miRNAs involved in the key stage of partly epiphyllous ovule germination in G. biloba var. epiphylla. Two small RNA libraries constructed from epiphyllous ovule leaves and normal leaves of G. biloba var. epiphylla were sequenced on an Illumina/Solexa platform. A total of 82 miRNA sequences belonging to 23 families and 53 putative novel miRNAs were identified in the two libraries. Differential expression analysis showed that 25 conserved and 21 novel miRNAs were differentially expressed between epiphyllous ovule leaves and normal leaves. The expression patterns of partially differentially expressed miRNAs and the transcript levels of their predicted target genes were validated by quantitative real time RT-PCR. All the expression profiles of the 21 selected miRNAs were similar to those detected by Solexa deep sequencing. Additionally, the transcript levels of almost all the putative target genes of 9 selected miRNAs were opposite to those of the corresponding miRNAs. The putative target genes of the differentially expressed miRNAs were annotated with Gene Ontology terms related to reproductive process, metabolic process and responding to stimulus. This work presents a broad range of small RNA transcriptome data obtained from epiphyllous ovule and normal leaves of G. biloba var. epiphylla, which may provide insights into the miRNA-mediated regulation in the epiphyllous ovule germination process.

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“…In the current study, 68 members of the FAD gene family have been founded in wheat that are significantly larger than the number of FAD genes in A. thaliana (25) [16], O. sativa (19) [33], Glycine max (29) [34], and Medicago trancatula (20) [35]. Therefore, the expansion of the FAD gene family is species-specific in different plants and this expansion is the result of gene duplication events [36].…”

Section: Discussionmentioning

confidence: 78%

“…Based on their results, the expression of GbFAD2 and GbSAD genes has been increased in 4 and 15 ᵒC, while it has been prevented in 35 and 45 ᵒC. In contrast, the expression of GbFAD6 was constant at different temperatures [19]. The expression of FAD2-1 and FAD2-2 genes of olive has been increased in response to wounding [20].…”

Section: Introductionmentioning

confidence: 99%

Identification, Evolution, Expression, and Docking Studies of Fatty Acid Desaturase Genes in Wheat (Triticum aestivum L.)

Hajiahmadi

Abedi

Wei

et al. 2020

Preprint

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Backgrounds: Fatty acid desaturases (FADs) introduce a double bond into the fatty acids acyl chain resulting in unsaturated fatty acids which have important roles in plant development and response to biotic and abiotic stresses. Wheat germ oil, one of the important by-products of wheat, can be a good alternative for edible oils with clinical advantages due to the high amount of unsaturated fatty acids. Therefore, genome-wide analysis of wheat FAD gene family (TaFADs) has been performed.Results: 68 FAD genes were identified from the wheat genome. Based on the phylogenetic analysis, wheat FADs clustered into five subfamilies, including FAB2, FAD2/FAD6, FAD4, DES/SLD, and FAD3/FAD7/FAD8. The TaFADs were distributed on chromosomes 2A-7B with 0 to 10 introns. The Ka/Ks ratio was less than one for most of the duplicated pair genes revealed that the function of the genes has been maintained during the evolution. Several cis-acting elements related to hormones and stresses in the TaFADs promoters indicated the role of these genes in plant development and responses to environmental stresses. Likewise, 72 SSRs and 91 miRNAs in 36 and 47 TaFADs have been identified. According to RNA-seq data analysis, the highest expression in all developmental stages and tissues was related to TaFAB2.5, TaFAB2.12, TaFAB2.15, TaFAB2.17, TaFAB2.20, TaFAD2.1, TaFAD2.6, and TaFAD2.8 genes while the highest expression in response to temperature stress was related to TaFAD2.6, TaFAD2.8, TaFAB2.15, TaFAB2.17, and TaFAB2.20. Furthermore, docking simulations revealed several residues in the active site of TaFAD2.6 and TaFAD2.8 in close contact with the docked oleic acid that could be useful in future site-directed mutagenesis studies to increase the catalytic efficiency of them and subsequently improve agronomic quality and tolerance of wheat against environmental stresses. Conclusions:This study provides comprehensive information that can lead to the detection of candidate genes for wheat genetic modification.

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Cloning and Expression of Stearoyl-ACP Desaturase and Two Oleate Desaturases Genes from Ginkgo biloba L.

Cited by 17 publications

References 62 publications

Transcriptome Profile Analysis from Different Sex Types of Ginkgo biloba L.

Transcriptome Profile Analysis from Different Sex Types of Ginkgo biloba L.

Identification and Characterization of MicroRNAs in Ginkgo biloba var. epiphylla Mak

Identification, Evolution, Expression, and Docking Studies of Fatty Acid Desaturase Genes in Wheat (Triticum aestivum L.)

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