Molecular cloning of Kazal-type proteinase inhibitor of the shrimp Fenneropenaeus chinensis

Kazal-type serine proteinase inhibitors from the black tiger shrimp Penaeus monodon and the inhibitory activities of SPIPm4 and 5

“…Since the blood circulation is the major site of immune defence system in shrimp, the notion that Kazal SPIs are involved in host defence innate immune response is also supported. This is in line with a recent finding that an SPI from F. chinensis is up-regulated in the hemocytes in response to white spot syndrome virus (WSSV) infection [18].…”

“…They are two-domain Kazal type inhibitors with the P1 residues Ile and Phe in SPIPm4 and Leu and Thr in SPIPm5. A recently isolated SPI from F. chinensis is found to be a SPIPm5 ortholog with different P1 amino acids in the second domain, Thr in SPIPm5 and Ala in SPI from F. chinensis [18]. The SPIPm4 and 5 were chosen in this study.…”

“…The two-domain Kazal-type SPI from the oriental white shrimp Fenneropenaeus chinensis was characterized. Its expression was up-regulated in the white spot syndrome virus (WSSV) infected shrimp [18].…”

Two Kazal-type protease inhibitors from Macrobrachium nipponense and Eriocheir sinensis: Comparative analysis of structure and activities

“…In crustaceans, numerous KPIs have been identified in recent years, most notably in the species Penaeus monodon [19,20], Litopenaeus vannamei [21], Fenneropenaeus chinensis [11,22] and Procambarus clarkii [23,24]. These KPIs are all expressed outside the male reproductive system and are believed to be involved in the innate immune response [25].…”

Three Kazal-type serine proteinase inhibitors from the red swamp crayfish Procambarus clarkii and the characterization, function analysis of hcPcSPI2

“…Several of these inhibitors are believed to be involved in innate immune response. For example, the two-domain and sevendomain Kazal-type SPIs from the Chinese white shrimp Fenneropenaeus chinensis were found to participate in anti-WSSV and anti-bacterial immune response, respectively [12,13]. In addition, the inhibitors SPIPm2 from the black tiger shrimp Penaeus monodon and hcPcSPI1 from the crayfish Procambarus clarkii, both showing inhibitory activity against subtilisin, are believed to defend against invading bacterial pathogens [14,15].…”