Two Kazal-type protease inhibitors from Macrobrachium nipponense and Eriocheir sinensis: Comparative analysis of structure and activities

Qian, Yeqing; Li, Ye; Yang, Fan; Yu, Yan-Qin; Yang, Jin‐Shu; Yang, Wei‐Jun

doi:10.1016/j.fsi.2011.12.006

Cited by 12 publications

(5 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Peptidase inhibitors were not only functional on cell migration, signal transmission, wound healing, and tissue remodeling [66], but also on male-reproduction in Drosophila [67]. Previous studies have confirmed that the male reproduction-related peptidase inhibitor Kazal-type (MRPINK) gene has a potential effect on male reproductive processes in prawns and crab [12,68,69]. In our study, one peptidase inhibitor (male reproductive tract-specific Kazal-type proteinase inhibitor) was expressed at higher levels in male gonads, suggesting that the peptidase inhibitor plays a critical role in gonadal development.…”

Section: Discussionmentioning

confidence: 95%

Comparative Transcriptome Analysis of Gonads for the Identification of Sex-Related Genes in Giant Freshwater Prawns (Macrobrachium Rosenbergii) Using RNA Sequencing

Jiang

Yuan

Qingqing

et al. 2019

Genes

View full text Add to dashboard Cite

The giant freshwater prawn (Macrobrachium rosenbergii) exhibits sex dimorphism between the male and female individuals. To date, the molecular mechanism governing gonadal development was unclear, and limited data were available on the gonad transcriptome of M. rosenbergii. Here, we conducted comprehensive gonadal transcriptomic analysis of female (ZW), super female (WW), and male (ZZ) M. rosenbergii for gene discovery. A total of 70.33 gigabases (Gb) of sequences were generated. There were 115,338 unigenes assembled with a mean size of 1196 base pair (bp) and N50 of 2195 bp. Alignment against the National Center for Biotechnology Information (NCBI) non-redundant nucleotide/protein sequence database (NR and NT), the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, SwissProt database, Protein family (Pfam), Gene ontology (GO), and the eukaryotic orthologous group (KOG) database, 36,282 unigenes were annotated at least in one database. Comparative transcriptome analysis observed that 10,641, 16,903, and 3393 genes were significantly differentially expressed in ZW vs. ZZ, WW vs. ZZ, and WW vs. ZW samples, respectively. Enrichment analysis of differentially expressed genes (DEGs) resulted in 268, 153, and 42 significantly enriched GO terms, respectively, and a total of 56 significantly enriched KEGG pathways. Additionally, 23 putative sex-related genes, including Gtsf1, IR, HSP21, MRPINK, Mrr, and other potentially promising candidate genes were identified. Moreover, 56,241 simple sequence repeats (SSRs) were identified. Our findings provide a valuable archive for further functional analyses of sex-related genes and future discoveries of underlying molecular mechanisms of gonadal development and sex determination. delicious flesh and high value nutrition. Like other crustaceans, M. rosenbergii displays sexual dimorphic growth pattern: The male individuals grow much larger and faster than the females [1,2]. Thus, based on the economic impact, developing the monosex population culture as the efficient approach to boost production has received great attention from researchers in M. rosenbergii. Nevertheless, the premise of monosex culture is to understand the molecular mechanism of sex determination, as well as to unravel sex-related genes.Studies on sex determination have proved that many macruran species exhibit the ZW/ZZ sex determination system [3][4][5]. In M. rosenbergii, sex reversal experiments proposed that the female prawns bear the ZW sex chromosomes, and the male individuals bear the ZZ chromosomes [1,6,7]. Subsequently, isolation of sex-specific markers further deepened the insight of the ZW/ZZ sex determination mode [8,9]. Recently, Ma et al. have confirmed that M. rosenbergii has the ZW/ZZ sex determination system based on the bacterial artificial chromosome (BAC) library, and nine genes were unraveled to be the key sex-linked genes by PacBio sequencing. Amongst, three genes, including zinc knuckle domain (ZKD), reverse transcriptase, and ANCDUO, protein homologs were known genes without characteri...

show abstract

Section: Discussionmentioning

confidence: 95%

Comparative Transcriptome Analysis of Gonads for the Identification of Sex-Related Genes in Giant Freshwater Prawns (Macrobrachium Rosenbergii) Using RNA Sequencing

Jiang

Yuan

Qingqing

et al. 2019

Genes

View full text Add to dashboard Cite

show abstract

“…A Kazal type serine protease inhibitor (AY885242) has been reported to have an inhibitory effect on gelatinolytic activity and proteolytic activity in M. rosenbergii in male reproductive system especially sperm cells (Qian et al . ).…”

Section: Immune Systemmentioning

confidence: 97%

Impacts of environmental and biological stressors on immune system ofMacrobrachium rosenbergii

Kumaresan

Palanisamy

Pasupuleti

et al. 2016

Reviews in Aquaculture

View full text Add to dashboard Cite

Macrobrachium rosenbergii commonly called giant freshwater prawn is a widely farmed crustacean in freshwater. Similar to other aquatic organisms, their growth and well-being is influenced by various physical, chemical and biological factors. We discuss about the critical growth limiting factors as well as disease causing agents and the potential immune molecules of M. rosenbergii that are proved to involve in preventing and/or responding to those limiting factors.

show abstract

“…(Kao et al, 2007. Furthermore, other study revelas that two male reproductive tract-derived Kazal-type protease inhibitors (KPIs) can completely inhibit the gelatinolytic activities of sperm proteases from their own species (M. nipponense and the Chinese mitten crab Eriocheir sinensis) (Qian et al, 2012). Otherwise, we identi ed serine proteases.…”

Section: Discussionmentioning

confidence: 97%

Transcriptome analysis of reproductive tract tissues of male river prawn Macrobrachium americanum

Martín-Manzo,

Morelos-Castro,

Munguia-Vega

et al. 2024

Mol Biol Rep

View full text Add to dashboard Cite

The river prawn, Macrobrachium americanum (M. americanum), is one of the largest prawns of the genus in Latin America and is an amphidromous species distributed along the Paci c coast of America. This prawn has commercial value due to its size and taste, making it a good option for aquaculture production. Its culture has been attempted in ponds and concrete tanks, but no successful technique can still support commercial production. Understanding the mechanisms that regulate reproduction at the molecular level is very important. This knowledge can provide tools for manipulating transcripts, which could increase the number or size of animals in the culture. However, our understanding of the mechanism that regulates the reproduction of M. americanum at the molecular level is limited. In this study, we performed transcriptome assembly of the testes, vas deferens, and terminal ampulla of M. americanum. The cDNA library was constructed and sequenced for each tissue to identify novel transcripts. A combined transcriptome with the three tissues was assembled using Trinity software.Unigenes were annotated using BLASTx and BLAST2GO. The transcriptome assembly generated 1,059,447 unigenes, of which 7,222 genes had signi cant hits (e-value < 1x10 − 5 ) when compared against the Swiss-Prot database. Around 75 genes were related to sex determination, testis development, spermatogenesis, spermiogenesis, fertilization, maturation of testicular cells, neuropeptides, hormones, hormone receptors, and/or embryogenesis. These results provide new molecular information about M. americanum reproduction, representing a reference point for further genetic studies of this species.

show abstract

Two Kazal-type protease inhibitors from Macrobrachium nipponense and Eriocheir sinensis: Comparative analysis of structure and activities

Cited by 12 publications

References 50 publications

Comparative Transcriptome Analysis of Gonads for the Identification of Sex-Related Genes in Giant Freshwater Prawns (Macrobrachium Rosenbergii) Using RNA Sequencing

Comparative Transcriptome Analysis of Gonads for the Identification of Sex-Related Genes in Giant Freshwater Prawns (Macrobrachium Rosenbergii) Using RNA Sequencing

Impacts of environmental and biological stressors on immune system ofMacrobrachium rosenbergii

Transcriptome analysis of reproductive tract tissues of male river prawn Macrobrachium americanum

Contact Info

Product

Resources

About