2003
DOI: 10.1128/jcm.41.8.3901-3904.2003
|View full text |Cite
|
Sign up to set email alerts
|

Molecular Cloning, Expression, Self-Assembly, Antigenicity, and Seroepidemiology of a Genogroup II Norovirus Isolated in France

Abstract: Virus-like particles of Dijon171/96 virus, a genogroup II norovirus, were expressed in a baculovirus system and were used for a seroepidemiological study of 1,078 age-stratified human sera collected in Dijon, France. The results showed a seroprevalence of 74.1%. Furthermore, we showed that murine antibodies generated against recombinant Dijon171/96 virus, and human antibodies recognized discontinuous epitopes on the particles.Norwalk-like viruses recently designated noroviruses (Caliciviridae family) represent… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

3
20
0

Year Published

2006
2006
2016
2016

Publication Types

Select...
7
1

Relationship

0
8

Authors

Journals

citations
Cited by 28 publications
(23 citation statements)
references
References 22 publications
3
20
0
Order By: Relevance
“…The NoV strains used for this study were isolated from patients suffering from gastroenteritis in France (i.e., Dijon171 and E1057 strains were US95/96 and Hunter GII.4 variants, respectively), Egypt (i.e., Cairo7, Cairo1, and Cairo4 isolates belonged to the Yerseke, Den Haag, and Osaka GII.4 variants, respectively), and the United States (i.e., the MD145 strain was representative of the Bristol variant), as documented previously (15,20,21,34) (Table 1).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The NoV strains used for this study were isolated from patients suffering from gastroenteritis in France (i.e., Dijon171 and E1057 strains were US95/96 and Hunter GII.4 variants, respectively), Egypt (i.e., Cairo7, Cairo1, and Cairo4 isolates belonged to the Yerseke, Den Haag, and Osaka GII.4 variants, respectively), and the United States (i.e., the MD145 strain was representative of the Bristol variant), as documented previously (15,20,21,34) (Table 1).…”
Section: Methodsmentioning
confidence: 99%
“…The Dijon171 recombinant baculovirus has been documented previously (34). The MD145 baculovirus was a kind gift of Kim Green (NIAID, NIH, Bethesda, MD).…”
Section: Methodsmentioning
confidence: 99%
“…The simplicity of the assay, which does not require labeling of the viral particles, combined with low material consumption, makes it attractive in pharmaceutics, where methods allowing for screening of new compounds inhibiting virus binding and spreading are highly desirable. The single viral particle sensitivity makes it also competitive with conventional quantitative polymerase-chain-reaction-based diagnostics [8].…”
mentioning
confidence: 99%
“…Although the concept is generic, we focus in this work on the norovirus genogroup II.4 Dijon strain [8][9][10] and its interaction with membranes containing the glycosphingolipids (GSLs) H type 1 and Lewis b [5(b)]. Norovirus is a nonenveloped RNA virus, 38 nm in diameter, with a symmetric icosahedral capsid assembled from 180 copies of the capsid protein arranged into 30 hexagonal facets [11] ( Fig.…”
mentioning
confidence: 99%
“…Since no information on the genetic characterization of norovirus infections in Portugal was available, we selected GII.4 New Orleans antigen as this GII.4 variant emerged in 2009 and continued to cause outbreaks in 2012 [13]. Compared to data from other countries, such as France which reported a prevalence of 74.1% [14]; Italy, 91.2% [15]; and the UK, 99.5% [8], the 70% we found in the Portuguese samples was relatively low. This variance could reflect real differences in exposure levels of norovirus in different European countries, or may be the result of the fact that the VLPs used in our study were unable to detect cross-reactive antibodies against non-GII.4 norovirus strains including GI [16].…”
Section: Discussionmentioning
confidence: 78%