Interaction of Single Viruslike Particles with Vesicles Containing Glycosphingolipids

Bally, Marta; Gunnarsson, Anders; Svensson, Lennart; Larson, Göran; Zhdanov, Vladimir P.; Höök, Fredrik

doi:10.1103/physrevlett.107.188103

Cited by 65 publications

(130 citation statements)

References 28 publications

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“…The fitted values of k off 1 and k off 2 from Equation 2 therefore have little meaning (38). Generally, for a Langmuir binding, the association rate constant k on can be determined using the relation K D ϭ k off /k on .…”

Section: Biological Activities Of Hsv-1 Mutant Lacking the Mucin-likementioning

confidence: 99%

“…Provided that binding is reaction-limited, this technique allows for an independent determination of k on (as further detailed in Ref. 38). The slope of the association curve (Fig.…”

Section: Biological Activities Of Hsv-1 Mutant Lacking the Mucin-likementioning

confidence: 99%

“…The liposomes were obtained by the hydration and extrusion method as described previously (38). This was followed by the sequential additions of 25 g/ml streptavidin and biotinylated GAGs (0.5 mg/ml for b-CS and 0.1 mg/ml for b-HA) for at least 20 min.…”

Section: Preparation Of Hsv-1 Variants Lacking the Mucin-like Domain mentioning

confidence: 99%

See 2 more Smart Citations

Mucin-like Region of Herpes Simplex Virus Type 1 Attachment Protein Glycoprotein C (gC) Modulates the Virus-Glycosaminoglycan Interaction

Altgärde

Eriksson

Peerboom

et al. 2015

Journal of Biological Chemistry

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Background: Herpes simplex virus attachment protein gC comprises a glycosaminoglycan-binding site and a mucin-like region. Results: Removal of the mucin-like region modifies gC interaction with glycosaminoglycans. Conclusion:The mucin-like region balances the gC-glycosaminoglycan interaction during virus binding to and release from target cells. Significance: The finding might be of relevance for similar proteins on other GAG-binding viruses.

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Section: Biological Activities Of Hsv-1 Mutant Lacking the Mucin-likementioning

confidence: 99%

“…Provided that binding is reaction-limited, this technique allows for an independent determination of k on (as further detailed in Ref. 38). The slope of the association curve (Fig.…”

Section: Biological Activities Of Hsv-1 Mutant Lacking the Mucin-likementioning

confidence: 99%

See 1 more Smart Citation

Mucin-like Region of Herpes Simplex Virus Type 1 Attachment Protein Glycoprotein C (gC) Modulates the Virus-Glycosaminoglycan Interaction

Altgärde

Eriksson

Peerboom

et al. 2015

Journal of Biological Chemistry

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“…23 Alternatively, suspended vesicles may interact with various species attached to a supported lipid bilayer. 24 In all these experiments, the kinetics were simultaneously tracked on a large number of individual fluorescent dye-labelled vesicles by using total internal reflection fluorescence microscopy (TIRFM) 17,20,21,24 or confocal fluorescence microscopy (CFM). 14,15,19,22,23 (Historically, TIRFM was introduced to study various surface bioprocesses in the early 1980s; 25,26 for CFM and combination of TIRFM and CFM, see Refs.…”

Section: Introductionmentioning

confidence: 99%

Total internal reflection fluorescence microscopy for determination of size of individual immobilized vesicles: Theory and experiment

Olsson

Zhdanov

Höök

2015

Journal of Applied Physics

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Lipid vesicles immobilized via molecular linkers at a solid support represent a convenient platform for basic and applied studies of biological processes occurring at lipid membranes. Using total internal reflection fluorescence microscopy (TIRFM), one can track such processes at the level of individual vesicles provided that they contain dyes. In such experiments, it is desirable to determine the size of each vesicle, which may be in the range from 50 to 1000 nm. Fortunately, TIRFM in combination with nanoparticle tracking analysis makes it possible to solve this problem as well. Herein, we present the formalism allowing one to interpret the TIRFM measurements of the latter category. The analysis is focused primarily on the case of unpolarized light. The specifics of the use of polarized light are also discussed. In addition, we show the expected difference in size distribution of suspended and immobilized vesicles under the assumption that the latter ones are deposited under diffusion-controlled conditions. In the experimental part of our work, we provide representative results, showing explicit advantages and some shortcomings of the use of TIRFM in the context under consideration, as well as how our refined formalism improves previously suggested approaches. V C 2015 AIP Publishing LLC. [http://dx

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“…Biased binding events were removed using a criterion described elsewhere (18). Briefly, binding events that started since the first frame of the movie and those that started after halfway through the movie were discarded due to the inability to determine actual binding times and oversampling of short binding events, respectively.…”

mentioning

confidence: 99%

Single-Particle Tracking Shows that a Point Mutation in the Carnivore Parvovirus Capsid Switches Binding between Host-Specific Transferrin Receptors

Lee

Allison

Bacon

et al. 2016

J Virol

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bDetermining how viruses infect new hosts via receptor-binding mechanisms is important for understanding virus emergence. We studied the binding kinetics of canine parvovirus (CPV) variants isolated from raccoons-a newly recognized CPV host-to different carnivore transferrin receptors (TfRs) using single-particle tracking. Our data suggest that CPV may utilize adhesion-strengthening mechanisms during TfR binding and that a single mutation in the viral capsid at VP2 position 300 can profoundly alter receptor binding and infectivity. Canine parvovirus (CPV) is a pathogen of dogs that emerged and caused a pandemic of disease in the 1970s and is Ͼ99% identical in nucleotide sequence to feline panleukopenia virus (FPV), a parvovirus that infects cats and other carnivore hosts but not dogs (1-3). Although the emergence of CPV has been presumed to be the result of a direct transfer of FPV or a similar virus from domestic cats to dogs, we recently demonstrated that CPV exists endemically in sylvatic cycles in North America involving a number of wild carnivore hosts, most notably raccoons (4, 5). These recent findings, along with the lack of isolation or detection of intermediate viruses between FPV and CPV from domestic animals, suggest that parvoviruses transfer frequently between domestic and wild carnivores and that the events preceding the pandemic emergence of CPV were more complex than previously believed (6).Although raccoons have long been known to be susceptible to FPV infection (7), they have only recently been identified as an important host for viruses that are closely related to CPV (4, 8). While CPVs from dogs, wolves, and coyotes all contain a Gly at capsid (VP2) position 300, CPVs from raccoons contain an Asp at that position, suggesting that this mutation is important for the adaptation of CPV to raccoons and possibly other wild carnivore hosts (4, 6). Additionally, VP2 position 300 is the most variable residue in the capsid (9-11). Since FPV and CPV capsids can bind to the transferrin receptor type 1 (TfR), in part by involving the structural region surrounding VP2 position 300 (12), the variations observed at this position appear to be selected by the unique TfR structures of individual carnivore hosts. To examine this phenomenon and to better understand the receptor-binding mechanisms involved, we used single-particle tracking (SPT) techniques to characterize the binding of raccoon-derived CPVs, containing either a 300-Asp or 300-Gly VP2 residue, to dog and raccoon TfRs.The virus studied here was the prototype CPV isolated from raccoons (CPV/Raccoon/VA/118-A/07, GenBank sequence accession number JN867610), which contains an Asp at VP2 position 300 and cannot be propagated in dog cells (4, 6). We refer to this virus as Rac118-300D. However, a single point mutation of the VP2 300-Asp (codon GAT) to a Gly (codon GGT) results in efficient dog cell infec- Citation Lee DW, Allison AB, Bacon KB, Parrish CR, Daniel S. 2016. Single-particle tracking shows that a point mutation in the carnivore parvovirus caps...

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Interaction of Single Viruslike Particles with Vesicles Containing Glycosphingolipids

Cited by 65 publications

References 28 publications

Mucin-like Region of Herpes Simplex Virus Type 1 Attachment Protein Glycoprotein C (gC) Modulates the Virus-Glycosaminoglycan Interaction

Mucin-like Region of Herpes Simplex Virus Type 1 Attachment Protein Glycoprotein C (gC) Modulates the Virus-Glycosaminoglycan Interaction

Total internal reflection fluorescence microscopy for determination of size of individual immobilized vesicles: Theory and experiment

Single-Particle Tracking Shows that a Point Mutation in the Carnivore Parvovirus Capsid Switches Binding between Host-Specific Transferrin Receptors

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