Molecular cloning and immunological characterization of phosphoglycerate kinase from Clonorchis sinensis

Song, Hong; Seong, Kee-Young; Sohn, Woon-Mok; Song, Kye‐Yong

doi:10.1016/s0166-6851(00)00220-6

Cited by 73 publications

(54 citation statements)

References 31 publications

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“…, Byung-Suk CHUNG 1) , Min-Ho CHOI 1) and Sung- Tae HONG 1) ferase were found mainly in the tegument (Hong et al, 2000;Kang et al, 2001). Moreover, purified 17-kDa protein was found in eggs and reproductive organs, and 21-kDa myoglobin of C. sinensis was found over the whole body, including the tegument, oral sucker and vitellaria (Chung et al, 2003;Sim et al, 2003).…”

Section: Organ-specific Antigens Of Clonorchis Sinensismentioning

confidence: 98%

Organ-specific antigens of Clonorchis sinensis

Chung

Choi

et al. 2004

Korean J Parasitol

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This study was carried out to find out specific proteins from different organs of Clonorchis sinensis. Crude extract, organ-specific and excretory-secretory (ES) proteins were analyzed by immunoblot with infected human sera. The bands of 7-and 17-kDa were main component of intestinal fluid and ES protein and commonly found in all organspecific proteins. The 17-kDa protein was observed from ES antigen, intestinal fluid, eggs and sperms, 26-and 28-kDa proteins were from the uterus, vitellaria, and ovary, and 34-, 37-, 43-and 50-kDa proteins were mainly from the testis and sperms. Serum of mice immunized with sperms reacted to the 50-kDa protein by immunoblotting and immunohistochemical staining showed a positive reaction at the seminal receptacle and seminiferous tubule. The present results show that the 7-kDa protein is a common antigen of every part or organ of C. sinensis, but different organs express their specific antigenic protein bands.

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Section: Organ-specific Antigens Of Clonorchis Sinensismentioning

confidence: 98%

Organ-specific antigens of Clonorchis sinensis

Chung

Choi

et al. 2004

Korean J Parasitol

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“…It is equally probably that the protection following vaccination results from the disruption of some interaction between surface-expressed enzyme and the host. A tegumental location for the enzyme in Clonorchis sinensis supports the latter hypothesis [149]. In most eukaryotes, phosphoglyceromutase requires a tightly bound 2,3-bisphospho-Dglycerate cofactor.…”

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confidence: 68%

Metabolic enzymes of helminth parasites: potential as drug targets

Timson¹

2015

CPPS

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Metabolic pathways which extract energy from carbon compounds are essential for an organism's survival. Therefore, inhibition of enzymes in these pathways represents a potential therapeutic strategy to combat parasitic infections. However, the high degree of similarity between host and parasite enzymes makes this strategy potentially difficult. Nevertheless, several existing drugs to treat infections by parasitic helminths (worms) target metabolic enzymes. These include the trivalent antimonials which target phosphofructokinase and Clorsulon which targets phosphoglycerate mutase and phosphoglycerate kinase. Glycolytic enzymes from a variety of helminths have been characterised biochemically, and some inhibitors identified. To date none of these inhibitors have been developed into therapies. Many of these enzymes are externalised from the parasite and so are also of interest in the development of potential vaccines. Less work has been done on tricarboxylic acid cycle enzymes and oxidative phosphorylation complexes. Again, while some inhibitors have been identified none have been developed into drug-like molecules. Barriers to the development of novel drugs targeting metabolic enzymes include the lack of experimentally determined structures of helminth enzymes, lack of direct proof that the enzymes are vital in the parasites and lack of cell culture systems for many helminth species. Nevertheless, the success of Clorsulon (which discriminates between highly similar host and parasite enzymes) should inspire us to consider making serious efforts to discover novel anthelminthics which target metabolic enzymes.

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“…Energy production in this fluke depends largely on glycolysis (Kang et al 1969;Hong et al 2000). Through glycolysis, it takes up approximately 1.13 mg glucose/h per gram wet weight, produces lactate, and forms several types of amino acids as the end product from exogenous glucose (Hong et al 2000;Han et al 1961).…”

Section: Discussionmentioning

confidence: 99%

Analysis of the genes expressed in Clonorchis sinensis adults using the expressed sequence tag approach

Lee

Park

et al. 2003

Parasitol Res

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Clonorchis sinensis is a biliary tract parasite, which infects over 30 million people in China, Korea and Southeast Asia through the ingestion of undercooked freshwater fish that harbour the infective metacercariae. The genes expressed in C. sinensis adults were identified in order to develop novel drugs, better diagnostics and vaccines for the parasite. The C. sinensis cDNA library was constructed and DNA sequencing was performed with 450 randomly selected clones. Four hundred and fifteen clones contained the amino-acid-encoding sequences. The functions of these genes could be assigned by DNA sequence homology. Basic Local Alignment Search Tool X analysis showed that 277 of the 415 clones were strongly matched ( P<10(-9)) to previously identified proteins, while the remaining 138 fell into the "no database match" category. Among the clones matching previously identified proteins, 220 putatively identified genes were sorted into seven functional categories. These included the genes associated with energy metabolism (38), gene expression/RNA metabolism (21), regulatory/signalling components (14), protein metabolism/sorting (98), the structure/cytoskeleton (29), membrane transporters (ten) and antigenic proteins (ten). The remaining 57 clones were not included in these categories. The dataset included the genes encoding the proteases, a lipid binding protein, the antigen proteins and the other genes of interest from a diagnostics, drug or vaccine development viewpoint. The present expressed sequence tag analysis proved to be an effective tool for examining gene expression and identified several important genes which increase and complement our knowledge of the biology of C. sinensis.

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Molecular cloning and immunological characterization of phosphoglycerate kinase from Clonorchis sinensis

Cited by 73 publications

References 31 publications

Organ-specific antigens of Clonorchis sinensis

Organ-specific antigens of Clonorchis sinensis

Metabolic enzymes of helminth parasites: potential as drug targets

Analysis of the genes expressed in Clonorchis sinensis adults using the expressed sequence tag approach

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