Organ-specific antigens of Clonorchis sinensis

Abstract

This study was carried out to find out specific proteins from different organs of Clonorchis sinensis. Crude extract, organ-specific and excretory-secretory (ES) proteins were analyzed by immunoblot with infected human sera. The bands of 7-and 17-kDa were main component of intestinal fluid and ES protein and commonly found in all organspecific proteins. The 17-kDa protein was observed from ES antigen, intestinal fluid, eggs and sperms, 26-and 28-kDa proteins were from the uterus, vitellaria, and ovary, and 34-, 37-, 43-and 50-kDa proteins were mainly from the testis and sperms. Serum of mice immunized with sperms reacted to the 50-kDa protein by immunoblotting and immunohistochemical staining showed a positive reaction at the seminal receptacle and seminiferous tubule. The present results show that the 7-kDa protein is a common antigen of every part or organ of C. sinensis, but different organs express their specific antigenic protein bands.

Molecular characterization and serodiagnosis analysis of a novel lysophospholipase from Clonorchis sinensis

“…Moreover, the recombinant protein could react with the sera from rats immunized with the ESAs. Previous studies have shown that a 5-20% gradient SDS-PAGE of ESAs of C. sinensis produce major protein bands including 26 kDa (Li et al 2004;Sim et al 2003). Our results manifested that the 26-kDa protein band might be the CsLysoPLA protein.…”

Bile components and amino acids affect survival of the newly excysted juvenile Clonorchis sinensis in maintaining media

“…After digesting the fish with artificial gastric juice (0.5% pepsin, pH 2.0), metacercariae were collected under a dissecting microscope (Li et al 2004a), washed three times in sterile physiological saline (phosphate-buffered saline, PBS), and stored in PBS at 4°C until use.…”

Differential gene expression profiling in human cholangiocarcinoma cells treated with Clonorchis sinensis excretory-secretory products

“…Various antigenic polypeptides, including cysteine protease, myoglobin, and glutathione S-transferase, have been isolated from adult worm ESP. These recombinant proteins expressed in bacteria interacted with the sera of C. sinensis-infected patients, suggesting that these proteins may be good serodiagnostic antigens for human clonorchiasis (Hong et al 2002;Kang et al 2004;Li et al 2004). ESPs from other liver flukes have been shown to inhibit lymphoproliferative responses to mitogens (Cervi and Masih 1997), to increase cell proliferation by stimulating cell cycle regulatory proteins (Thuwajit et al 2004), and to induce eosinophil apoptosis, a process mediated by the activation of tyrosine kinases and caspase cascade (Serradell et al 2007).…”