Molecular cloning and expression of an encoding galactinol synthase gene (AnGolS1) in seedling of Ammopiptanthus nanus

Abstract

Based on the galactinol synthase (AnGolS1) fragment sequence from a cold-induced Suppression Subtractive Hybridization (SSH) library derived from Ammopiptanthus nanus (A. nanus) seedlings, AnGolS1 mRNA (including the 5′ UTR and 3′ UTR) (GenBank accession number: GU942748) was isolated and characterized by rapid amplification of cDNA ends polymerase chain reaction (RACE–PCR). A substrate reaction test revealed that AnGolS1 possessed galactinol synthase activity in vitro and could potentially be an early-responsive gene. Furthermore, quantitative real-time PCR (qRT-PCR) indicated that AnGolS1 was responded to cold, salts and drought stresses, however, significantly up-regulated in all origans by low temperatures, especially in plant stems. In addition, the hybridization signals in the fascicular cambium were strongest in all cells under low temperature. Thus, we propose that AnGolS1 plays critical roles in A. nanus low-temperature stress resistance and that fascicular cambium cells could be involved in AnGolS1 mRNA transcription, galactinol transportation and coordination under low-temperature stress.

Comparative transcriptomics provides novel insights into the mechanisms of selenium tolerance in the hyperaccumulator plant Cardamine hupingshanensis

“…The gene for transcription factor LBD 16 of the LBD family, which plays crucial roles in diverse growth and development processes, including the establishment and maintenance of the developmental boundary of lateral organs 31 , was up-regulated in the roots, indicating that selenium could promote lateral root formation in plants. AtGoLS3 is a member of the galactinol synthase (GolS) family, which initiates the biosynthesis of raffinose oligosaccharides (RFO), may act as an osmoprotectant in drought stress tolerance through UDP-galactose 32 , 33 . According to results of RNA-Seq, a novel possibility is that AtGoLS3 is induced not only by cold stress but also by selenium stress.…”

Herbivore exposure alters ion fluxes and improves salt tolerance in a desert shrub

“…A. nanus grows in a desert region of northwest China with saline‐sodic soils. The extreme climate and harsh living conditions in this area have caused A. nanus to adapt to be tolerant of various stresses (Liu et al, ; Lu et al, ; Yu et al, ). Several tolerance genes from A. nanus have previously been used to improve tolerance to environmental stresses by other species.…”

“…To adapt to harsh environments, A. nanus has evolved complex defensive mechanisms that protect it from drought, salt, alkali, extreme temperature, and insect feeding. In the past decade, several efforts have been made to elucidate the defensive patterns of A. nanus responses to abiotic or biotic stresses (Liu et al, 2016;Lu et al, 2010;Yu et al, 2015;Yu et al, 2016;Yu et al, 2017). (Bonaventure, VanDoorn, & Baldwin, 2011;Fürstenberg-Hägg, Zagrobelny, & Bak, 2013;Kessler & Baldwin, 2002;Zebelo & Maffei, 2015).…”

Magnaporthe oryzae Systemic Defense Trigger 1 (MoSDT1)-Mediated Metabolites Regulate Defense Response in Rice

“…Dmannose is mainly involved in synthesizing ascorbic acid, which is an antioxidant and cell reductant, and the improvement of antioxidant capacity is one of the characteristics of plant resistance [20]. Galactinol is also involved in inducing resistance in plant systems [21]. Glyceric acid is up-regulated in Rhizoctonia solani-infected resistant rice and participates in photorespiration and plant defense response regulation [9].…”

“…Firstly, rice leaves were inoculated with spores of M. oryzae strain 95234I-1b, and then sprayed with exogenous compounds after 72 h at three concentrations (Supplementary Table 2), and the concentrations of six compounds were prepared [21,24,26]. Disease index =∑ (number of leaves at each grade × representative values at all grades)/(review total leaf number × highest grade representative value)×100 [18].…”