Comparative transcriptomics provides novel insights into the mechanisms of selenium tolerance in the hyperaccumulator plant Cardamine hupingshanensis

Zhou, Yang; Qiao-yu, Tang; Wu, Meiru; Mou, Di; Liu, Hui; Wang, Shouchuang; Zhang, Chi; Li, Ding; Luo, Jie

doi:10.1038/s41598-018-21268-2

Cited by 49 publications

(39 citation statements)

References 80 publications

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“…The form of supplied Se may also have a significant effect on the response of a HA. A recent transcriptomic analysis of the Se HA Cardamine hupingshanensis found that, when supplied with selenite, the HA in fact downregulated the expression of its SULTR1;2 homolog ( Zhou et al, 2018 ). This result may be attributable to the fact that selenite is not likely to be transported through SULTRs, but rather through Pi transporters, due to the greater similarity of Pi to selenite ( White, 2018 ).…”

Section: Selenium Hyperaccumulatorsmentioning

confidence: 99%

Selenium transport and metabolism in plants: Phytoremediation and biofortification implications

Trippe

Pilon‐Smits

2021

Journal of Hazardous Materials

133

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The aim of this review is to synthesize current knowledge of selenium (Se) transport and metabolism in plants, with a focus on implications for biofortification and phytoremediation. Selenium is a necessary human micronutrient, and around a billion people worldwide may be Se deficient. This can be ameliorated by Se biofortification of staple crops. Selenium is also a potential toxin at higher concentrations, and multiple environmental disasters over the past 50 years have been caused by Se pollution from agricultural and industrial sources. Phytoremediation by plants able to take up large amounts of Se is an important tool to combat pollution issues. Both biofortification and phytoremediation applications require a thorough understanding of how Se is taken up and metabolized by plants. Selenium uptake and translocation in plants are largely accomplished via sulfur (S) transport proteins. Current understanding of these transporters is reviewed here, and transporters that may be manipulated to improve Se uptake are discussed. Plant Se metabolism also largely follows the S metabolic pathway. This pathway is reviewed here, with special focus on genes that have been, or may be manipulated to reduce the accumulation of toxic metabolites or enhance the accumulation of nontoxic metabolites. Finally, unique aspects of Se transport and metabolism in Se hyperaccumulators are reviewed. Hyperaccumulators, which can accumulate Se at up to 1000 times higher concentrations than normal plants, present interesting specialized systems of Se transport and metabolism. Selenium hyperaccumulation mechanisms and potential applications of these mechanisms to biofortification and phytoremediation are presented.

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Section: Selenium Hyperaccumulatorsmentioning

confidence: 99%

Selenium transport and metabolism in plants: Phytoremediation and biofortification implications

Trippe

Pilon‐Smits

2021

Journal of Hazardous Materials

133

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“…All clean reads of Illumina RNA-seq corresponding to each pooled sample were mapped against the assembled reference transcriptome using bowtie 2 (V2.1.0). The FPMK (fragments per Kilobase Million) values of each isoform were computed for each pooled sample and normalized using the RPKM (RSEM 1.2.15) [59].…”

Section: Quantification Of Gene Expression Levelsmentioning

confidence: 99%

Full-Length Transcriptome Assembly of Italian Ryegrass Root Integrated with RNA-Seq to Identify Genes in Response to Plant Cadmium Stress

Zhang

et al. 2020

IJMS

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Cadmium (Cd) is a toxic heavy metal element. It is relatively easily absorbed by plants and enters the food chain, resulting in human exposure to Cd. Italian ryegrass (Lolium multiflorum Lam.), an important forage cultivated widely in temperate regions worldwide, has the potential to be used in phytoremediation. However, genes regulating Cd translocation and accumulation in this species are not fully understood. Here, we optimized PacBio ISO-seq and integrated it with RNA-seq to construct a de novo full-length transcriptomic database for an un-sequenced autotetraploid species. With the database, we identified 2367 differentially expressed genes (DEGs) and profiled the molecular regulatory pathways of Italian ryegrass with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis in response to Cd stress. Overexpression of a DEG LmAUX1 in Arabidopsis thaliana significantly enhanced plant Cd concentration. We also unveiled the complexity of alternative splicing (AS) with a genome-free strategy. We reconstructed full-length UniTransModels using the reference transcriptome, and 29.76% of full-length models had more than one isoform. Taken together, the results enhanced our understanding of the genetic diversity and complexity of Italian ryegrass under Cd stress and provided valuable genetic resources for its gene identification and molecular breeding.

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“…To date, studies on Se-tolerance mechanisms have focused on some Se-hyperaccumulating plants (Freeman et al, 2010; Sabbagh & Van Hoewyk, 2012; Cappa et al, 2015). In Cardamine hupingshanensis seedlings, the expression of the sulfite oxidase ( SOX ) gene in the roots is up-regulated after the addition of selenite, thus indicating that selenite may first be converted to selenate, and then the selenate is metabolized (Zhou et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomic analysis of pepper (Capsicum annuum L.) seedlings under selenium stress

Zhang

Geng

et al. 2019

PeerJ

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Selenium (Se) is an essential trace element for human and animal health. Se fertilizer has been used to increase the Se content in crops to meet the Se requirements in humans and animals. To address the challenge of Se poisoning in plants, the mechanisms underlying Se-induced stress in plants must be understood. Here, to elucidate the effects of Se stress on the protein levels in pepper, we used an integrated approach involving tandem mass tag labeling, high performance liquid chromatography fractionation, and mass spectrometry-based analysis. A total of 4,693 proteins were identified, 3,938 of which yielded quantitative information. Among them, the expression of 172 proteins was up-regulated, and the expression of 28 proteins was down-regulated in the Se/mock treatment comparison. According to the above data, we performed a systematic bioinformatics analysis of all identified proteins and differentially expressed proteins (DEPs). The DEPs were most strongly associated with the terms “metabolic process,” “posttranslational modification, protein turnover, chaperones,” and “protein processing in endoplasmic reticulum” according to Gene Ontology, eukaryotic orthologous groups classification, and Kyoto Encyclopedia of Genes and Genomes enrichment analysis, respectively. Furthermore, several heat shock proteins were identified as DEPs. These results provide insights that may facilitate further studies on the pepper proteome expressed downstream of the Se stress response. Our data revealed that the responses of pepper to Se stress involve various pathways.

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Comparative transcriptomics provides novel insights into the mechanisms of selenium tolerance in the hyperaccumulator plant Cardamine hupingshanensis

Cited by 49 publications

References 80 publications

Selenium transport and metabolism in plants: Phytoremediation and biofortification implications

Selenium transport and metabolism in plants: Phytoremediation and biofortification implications

Full-Length Transcriptome Assembly of Italian Ryegrass Root Integrated with RNA-Seq to Identify Genes in Response to Plant Cadmium Stress

Comparative proteomic analysis of pepper (Capsicum annuum L.) seedlings under selenium stress

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