Molecular cloning and expression analysis of two sex-lethal homolog genes during development in the oriental river prawn, Macrobrachium nipponense

Abstract

ABSTRACT. In this study, two Sxl gene homologs, designated as Mnsxl1 and Mnsxl2, were cloned and characterized from the freshwater prawn Macrobrachium nipponense by rapid amplification of cDNA ends. The deduced amino acid sequences of Mnsxl1 and Mnsxl2 showed high sequence homology to the insect Sxl and contained conserved domains in two RNA-binding motifs. Real-time quantitative reverse transcription-polymerase chain reaction (RT-QPCR) showed that the Mnsxl1 and Mnsxl2 genes were expressed in all investigated tissues, with the highest level of expression in the intestine and liver. RT-QPCR also revealed that Mnsxl1 and Mnsxl2 mRNAs expressions were both significantly increased at 5 and 20 days post-larvae after metamorphosis. Thus, the results of the present study imply that Mnsxl1 and Mnsxl2 play

Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase

“…RT-qPCR measured the highest expression at stage PL10. In our previous study, we found that M. nipponense primordial germ cells (PGCs) appear between stages PL10 and PL15, together with external sexual characteristics (Zhang et al, 2013). Thus, the current results are consistent with these observations, suggesting that MnNOS may be involved in organ formation.…”

“…Melting curve analysis was performed from 65°-95°C in 10-s0. 5°C increments to verify the amplification of a single product (Zhang et al, 2013).…”

Molecular cloning and expression analysis of female sterile homeotic gene (fsh) in the oriental river prawn Macrobrachium nipponense

“…After metamorphosis, the expression of Mnfsh also began to increase in PL1. It is commonly know that, in the molting process, crustaceans experience morphological and appendage characteristic changes (Magalhães, 1989;Zhang et al, 2012). Therefore, our results indicate that the Mnfsh gene has a close relationship with metamorphosis of oriental river prawn.…”

“…Previous studies showed that zygotic mutations cause either lethality or female sterility, whereas maternal mutations cause segmental deletions and thoracic homeotic transformations (Rhee et al, 1998;Florence and Faller, 2008). Considering that crustaceans have a dramatically close evolutionary relationship with insects (Budd and Telford, 2009;Zhang et al, 2012), we hypothesize that Mnfsh is involved in the regulation of embryonic development in crustaceans.…”

“…This species is considered an important fishery resource in China, with an annual production of 230,248 tons (Bureau of Fishery, Ministry of Agriculture, and P.R.C., 2011). In recent years, our laboratory has performed some molecular research on the M. nipponense development system (Zhang et al, 2012(Zhang et al, , 2013a(Zhang et al, ,b, 2014. To further understand the molecular mechanism of maturation, other gene regulations involved in the developmental process also need to be examined in M. nipponense.…”