Molecular cloning and expression analysis of female sterile homeotic gene (fsh) in the oriental river prawn Macrobrachium nipponense

Zhang, W Y; Jiang, Sufei; Xiong, Yiwei; Fu, Hongtuo; Qiao, Hui; Sun, Shengming; Gong, Yongsheng; Jin, Shubo

doi:10.4238/2015.april.30.4

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…In M. nipponense, it was demonstrated that sh is related to histodifferentiation and organogenesis during the embryonic developmental process. While inex 3, that was described in D. melanogaster, has a role in embryogenesis (Zhang et al, 2015) KEGG analysis revealed that Necroptosis, NOD-like receptor signaling pathway and apoptosis were the most abundant metabolic pathways in the reproductive tract of M. americanum. Necroptosis is a form of regulated necrotic cell death.…”

Section: Discussionmentioning

confidence: 98%

Transcriptome analysis of reproductive tract tissues of male river prawn Macrobrachium americanum

Martín-Manzo,

Morelos-Castro,

Munguia-Vega

et al. 2024

Mol Biol Rep

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The river prawn, Macrobrachium americanum (M. americanum), is one of the largest prawns of the genus in Latin America and is an amphidromous species distributed along the Paci c coast of America. This prawn has commercial value due to its size and taste, making it a good option for aquaculture production. Its culture has been attempted in ponds and concrete tanks, but no successful technique can still support commercial production. Understanding the mechanisms that regulate reproduction at the molecular level is very important. This knowledge can provide tools for manipulating transcripts, which could increase the number or size of animals in the culture. However, our understanding of the mechanism that regulates the reproduction of M. americanum at the molecular level is limited. In this study, we performed transcriptome assembly of the testes, vas deferens, and terminal ampulla of M. americanum. The cDNA library was constructed and sequenced for each tissue to identify novel transcripts. A combined transcriptome with the three tissues was assembled using Trinity software.Unigenes were annotated using BLASTx and BLAST2GO. The transcriptome assembly generated 1,059,447 unigenes, of which 7,222 genes had signi cant hits (e-value < 1x10 − 5 ) when compared against the Swiss-Prot database. Around 75 genes were related to sex determination, testis development, spermatogenesis, spermiogenesis, fertilization, maturation of testicular cells, neuropeptides, hormones, hormone receptors, and/or embryogenesis. These results provide new molecular information about M. americanum reproduction, representing a reference point for further genetic studies of this species.

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Section: Discussionmentioning

confidence: 98%

Transcriptome analysis of reproductive tract tissues of male river prawn Macrobrachium americanum

Martín-Manzo,

Morelos-Castro,

Munguia-Vega

et al. 2024

Mol Biol Rep

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“…Although many molecular studies have investigated the reproduction, sexual control, stress, and nutrition in M. nipponense [17,18,19], most qPCR studies were performed using reference genes from other similar species without identification and verification, potentially leading to inaccurate results. It is therefore essential to screen for suitable specific reference genes in M. nipponense under different experimental conditions.…”

Section: Introductionmentioning

confidence: 99%

Validation and Evaluation of Reference Genes for Quantitative Real-Time PCR in Macrobrachium Nipponense

Qiao

et al. 2018

IJMS

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Quantitative real-time PCR (qPCR) is widely used in molecular biology, although the accuracy of the quantitative results is determined by the stability of the reference genes used. Recent studies have investigated suitable reference genes for some crustaceans under various conditions, but studies in Macrobrachium nipponense are currently lacking. In this study, we selected the following seven genes from among 35 commonly used housekeeping genes as candidate qPCR reference genes for temporal and spatial expression: EIF (eukaryotic translation initiation factor 5A), 18S (18S ribosomal RNA), EF-1α (elongation factor-1α), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), TUB (α-tubulin), β-act (β-actin), and RPL18 (Ribosomal protein L18). The stability of each reference gene was evaluated by GeNorm, NormFinder, BestKeeper, and comparative ∆C t methods, and was comprehensively ranked using RefFinder. RPL18 was shown to be the most suitable reference gene for adult M. nipponense tissues, while EIF was the most stable in different ovarian and embryo stages and in white spot syndrome virus infection, and β-act was the most stable reference gene under hypoxia stress. The reliability of the rankings was confirmed by RNA interference experiments. To the best of our knowledge, this represents the first systematic analysis of reference genes for qPCR experiments in M. nipponense, and the results will provide invaluable information for future research in closely related crustaceans.

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Comparative Transcriptome Analysis Provides Insights into Differentially Expressed Genes and Long Non-Coding RNAs between Ovary and Testis of the Mud Crab (Scylla paramamosain)

Yang

Ikhwanuddin

et al. 2017

Mar Biotechnol

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The molecular mechanism underlying sex determination and gonadal differentiation of the mud crab (Scylla paramamosain) has received considerable attention, due to the remarkably biological and economic differences between sexes. However, sex-biased genes, especially non-coding genes, which account for these differences, remain elusive in this crustacean species. In this study, the first de novo gonad transcriptome sequencing was performed to identify both differentially expressed genes and long non-coding RNAs (lncRNAs) between male and female S. paramamosain by using Illumina Hiseq2500. A total of 79,282,758 and 79,854,234 reads were generated from ovarian and testicular cDNA libraries, respectively. After filtrating and de novo assembly, 262,688 unigenes were produced from both libraries. Of these unigenes, 41,125 were annotated with known protein sequences in public databases. Homologous genes involved in sex determination and gonadal development pathways (Sxl-Tra/Tra-2-Dsx/Fru, Wnt4, thyroid hormone synthesis pathway, etc.) were identified. Three hundred and sixteen differentially expressed unigenes were further identified between both transcriptomes. Meanwhile, a total of 233,078 putative lncRNAs were predicted. Of these lncRNAs, 147 were differentially expressed between sexes. qRT-PCR results showed that nine lncRNAs negatively regulated the expression of eight genes, suggesting a potential role in sex differentiation. These findings will provide fundamental resources for further investigation on sex differentiation and regulatory mechanism in crustaceans.

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Molecular cloning and expression analysis of female sterile homeotic gene (fsh) in the oriental river prawn Macrobrachium nipponense

Cited by 4 publications

References 31 publications

Transcriptome analysis of reproductive tract tissues of male river prawn Macrobrachium americanum

Transcriptome analysis of reproductive tract tissues of male river prawn Macrobrachium americanum

Validation and Evaluation of Reference Genes for Quantitative Real-Time PCR in Macrobrachium Nipponense

Comparative Transcriptome Analysis Provides Insights into Differentially Expressed Genes and Long Non-Coding RNAs between Ovary and Testis of the Mud Crab (Scylla paramamosain)

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