Molecular cloning and characterization of a novel low temperature-induced gene, blti2, from barley (Hordeum vulgare L.)

Bahn, Sung Chul; Bae, Min Seok; Park, Yong Bum; Oh, Seung Ick; Jeung, Ji Ung; Bae, Jung Myung; Chung, Young Soo; Shin, Jeong Sheop

doi:10.1016/s0167-4781(01)00317-7

Cited by 10 publications

(6 citation statements)

References 16 publications

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“…More recently, Luo et al (2002) constructed an SSH cDNA library from wheat leaves inoculated by Erysiphe graminis DC to isolate genes that were involved in the powdery mildew resistance in wheat. Bahn et al (2001) screened differentially expressed genes from low temperature induced winter barley. Some genes had been cloned using the SSH technique (Yoshimura et al 1998;Kim et al 1999aKim et al , 1999bBahn et al 2001;Zhang et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Screening and analysis of differentially expressed genes from an alien addition line of wheatThinopyrum intermediuminduced bybarley yellow dwarf virusinfection

Jiang

Zhang²,

Hu³

et al. 2004

Genome

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The alien addition line TAI-27 contains a pair of chromosomes of Thinopyrum intermedium that carry resistance against barley yellow dwarf virus (BYDV). A subtractive library was constructed using the leaves of TAI-27, which were infected by Schizaphis graminum carrying the GAV strain of BYDV, and the control at the three-leaf stage. Nine differentially expressed genes were identified from 100 randomly picked clones and sequenced. Two of the nine clones were highly homologous with known genes. Of the remaining seven cDNA clones, five clones matched with known expressed sequence tag (EST) sequences from wheat and (or) barley whereas the other two clones were unknown. Five of the nine differentially expressed sequences (WTJ9, WTJ11, WTJ15, WTJ19, and WTJ32) were highly homologous (identities >94%) with ESTs from wheat or barley challenged with pathogens. These five sequences and another one (WTJ18) were also highly homologous (identities >86%) with abiotic stress induced ESTs in wheat or barley. Reverse Northern hybridization showed that seven of the nine differentially expressed cDNA sequences hybridized with cDNA of T. intermedium infected by BYDV. Three of these also hybridized with cDNA of line 3B-2 (a parent of TAI-27) infected by BYDV. The alien chromosome in TAI-27 was microdissected. The second round linker adaptor mediated PCR products of the alien chromosomal DNA were labeled with digoxygenin and used as the probe to hybridize with the nine differentially expressed genes. The analysis showed that seven differentially expressed genes were homologous with the alien chromosome of TAI-27. These seven differentially expressed sequences could be used as ESTs of the alien chromosome of TAI-27. This research laid the foundation for screening and cloning of new specific functional genes conferring resistance to BYDV and probably other pathogens.

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Section: Introductionmentioning

confidence: 99%

Screening and analysis of differentially expressed genes from an alien addition line of wheatThinopyrum intermediuminduced bybarley yellow dwarf virusinfection

Jiang

Zhang²,

Hu³

et al. 2004

Genome

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“…We compared the gene expression of O. cincta with high plasticity for juvenile growth with those with low plasticity after exposure to a temperature switch composed of a cooling or a warming treatment. Although SSH has been applied in many studies to identify environmentally regulated gene transcripts (Bahn et al, 2001;Gracey et al, 2001;Pereboom et al, 2005), a serious concern is the occurrence of false positives among the SSH-derived clones (randomly cloned cDNAs that do not respond to the treatment). Therefore, we removed false positives by confirming differential expression using quantitative real time-polymerase chain reaction (RT-PCR).…”

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confidence: 99%

Temperature‐induced gene expression associated with different thermal reaction norms for growth rate

et al. 2007

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Although nearly all organisms are subject to fluctuating temperature regimes in their natural habitat, little is known about the genetics underlying the response to thermal conditions, and even less about the genetic differences that cause individual variation in thermal response. Here, we aim to elucidate possible pathways involved in temperature-induced phenotypic plasticity of growth rate. Our model organism is the collembolan Orchesella cincta that occurs in a wide variety of habitats and is known to be adapted to local thermal conditions. Because sequence information is lacking in O. cincta, we constructed cDNA libraries enriched for temperature-responsive genes using suppression subtractive hybridization. We compared gene expression of O. cincta with steep thermal reaction norms (high plasticity) to those with flat thermal reaction norms (low plasticity) for juvenile growth after exposure to a temperature switch composed of a cooling or a warming treatment. Using suppression subtractive hybridization, we found differential expression of ten nuclear genes, including several genes involved in energy metabolism, such as pantothenate kinase and carbonic anhydrase. In addition, seven mitochondrial genes were found in the cloned subtracted library, but further analysis showed this was caused by allelic variation in mitochondrial genes in our founder population, and that a specific haplotype was associated with high thermal responsiveness. Future work will focus on candidate genes from pathways such as the oxidative phosphorylation and biosynthesis of coenzyme A which are possibly involved in thermal responsiveness of juvenile growth rate.

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“…15,16,27) The most important feature of SSH is capable of producing specific fragments and is helpful for inter-species identification. 28,29) It was first used for authentication of populations of this rare and endangered medicinal herbs and populationspecific sequences could be found from the whole genome with good reproducibility. GGL population was identified from JSR population and the other six populations by diagnostic primers (SSH-JB01, SSH-JB02) from the populationspecific sequence.…”

Section: Discussionmentioning

confidence: 99%

SNP, ARMS and SSH Authentication of Medicinal Dendrobium officinale KIMURA et MIGO and Application for Identification of Fengdou Drugs

Ding

Zhang

Feng

et al. 2008

Biological & Pharmaceutical Bulletin

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Molecular cloning and characterization of a novel low temperature-induced gene, blti2, from barley (Hordeum vulgare L.)

Cited by 10 publications

References 16 publications

Screening and analysis of differentially expressed genes from an alien addition line of wheatThinopyrum intermediuminduced bybarley yellow dwarf virusinfection

Screening and analysis of differentially expressed genes from an alien addition line of wheatThinopyrum intermediuminduced bybarley yellow dwarf virusinfection

Temperature‐induced gene expression associated with different thermal reaction norms for growth rate

SNP, ARMS and SSH Authentication of Medicinal Dendrobium officinale KIMURA et MIGO and Application for Identification of Fengdou Drugs

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