SNP, ARMS and SSH Authentication of Medicinal Dendrobium officinale KIMURA et MIGO and Application for Identification of Fengdou Drugs

Ding, Ge; Zhang, Dai-Zhen; Feng, Zhenyu; Fan, Wenjing; Ding, Xiaoyu; Li, Xuexia

doi:10.1248/bpb.31.553

Cited by 24 publications

(12 citation statements)

References 19 publications

(17 reference statements)

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“…2). D. officinale is one of the most popular and rare medicinal Dendrobium species in China [27], and D. tosaense was thought to be distributed mainly in Japan and Taiwan [28]. Based on morphological and biogeographic information, some researchers have regarded them as two different species [6,28,29], while Wood saw them as a common species [1].…”

Section: Discussionmentioning

confidence: 99%

Identification of medicinal Dendrobium species by phylogenetic analyses using matK and rbcL sequences

et al. 2010

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Species identification of five Dendrobium plants was conducted using phylogenetic analysis and the validity of the method was verified. Some Dendrobium plants (Orchidaceae) have been used as herbal medicines but the difficulty in identifying their botanical origin by traditional methods prevented their full modern utilization. Based on the emerging field of molecular systematics as a powerful classification tool, a phylogenetic analysis was conducted using sequences of two plastid genes, the maturase-coding gene (matK) and the large subunit of ribulose 1,5-bisphosphate carboxylase-coding gene (rbcL), as DNA barcodes for species identification of Dendrobium plants. We investigated five medicinal Dendrobium species, Dendrobium fimbriatum, D. moniliforme, D. nobile, D. pulchellum, and D. tosaense. The phylogenetic trees constructed from matK data successfully distinguished each species from each other. On the other hand, rbcL, as a single-locus barcode, offered less species discriminating power than matK, possibly due to its being present with little variation. When results using matK sequences of D. officinale that was deposited in the DNA database were combined, D. officinale and D. tosaense showed a close genetic relationship, which brought us closer to resolving the question of their taxonomic identity. Identification of the plant source as well as the uniformity of the chemical components is critical for the quality control of herbal medicines and it is important that the processed materials be validated. The methods presented here could be applied to the analysis of processed Dendrobium plants and be a promising tool for the identification of botanical origins of crude drugs.

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Section: Discussionmentioning

confidence: 99%

Identification of medicinal Dendrobium species by phylogenetic analyses using matK and rbcL sequences

et al. 2010

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“…[16][17][18][19][20][21][22][23][24][25][26][27] Unfortunately, these methods have only been applied to a limited number of Dendrobium species, and molecular markers that can be applied more universally to Dendrobium have not been developed. To recognize herbal medicines accurately at the species level, a comprehensive DNA sequence database that covers Dendrobium species potentially used for medicine is desired, and to create such a database, the following conditions are required: (1) precise identification of the plant species, (2) comprehensive data banking of reference sequences for the relevant plant group at the species level, and (3) employment of sequence markers that can discriminate interspecific differences.…”

mentioning

confidence: 99%

Identification of Dendrobium Species Used for Herbal Medicines Based on Ribosomal DNA Internal Transcribed Spacer Sequence

Takamiya

Wongsawad

Tajima

et al. 2011

Biological & Pharmaceutical Bulletin

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Dendrobium is one of the largest genera in the Orchidaceae, of which about 1200 species are distributed widely in the Asia-Pacific region. 1,2) Owing to the enormous number of species and great diversification of morphological characters, many taxonomic and phylogenetic problems of this genus remain unresolved.Dried stems of specific Dendrobium species, so-called Dendrobii Herba, have been used in traditional Chinese medicine for benefiting the stomach, promoting the production of body fluid, nourishing "yin," and antipyresis.3,4) Stems of some Dendrobium species contain compounds that exhibit antioxidant activity and antitumor activity.5-7) Accordingly, the demand for Dendrobii Herba has increased rapidly, and the wild resources of Dendrobium have decreased severely due to over-collection. Seventy-four Dendrobium species are distributed in China. However, in practice, Dendrobium species that are not described in either of these references are used clinically, and other orchid genera such as Pholidota have also been used as adulterants.9-11) Such a lax situation in commercial distribution could result in the risk of inconsistent therapeutic effect and even be a threat against consumer safety. Therefore, discrimination of Dendrobii Herba is important for quality control. However, it is difficult to identify Dendrobium species from dried stems by traditional methods based on morphological, anatomical and/or chemical analyses [12][13][14] because these characteristics are not always useful markers at the species level and often vary depending on growing conditions.To date, several molecular analytical methods have been applied to identify herbal medicines.15) For example, DNA sequencing, DNA microarrays, random amplified polymorphic DNA (RAPD), and DNA fingerprinting have been used for identification of Dendrobium species. [16][17][18][19][20][21][22][23][24][25][26][27] Unfortunately, these methods have only been applied to a limited number of Dendrobium species, and molecular markers that can be applied more universally to Dendrobium have not been developed. To recognize herbal medicines accurately at the species level, a comprehensive DNA sequence database that covers Dendrobium species potentially used for medicine is desired, and to create such a database, the following conditions are required: (1) precise identification of the plant species, (2) comprehensive data banking of reference sequences for the relevant plant group at the species level, and (3) employment of sequence markers that can discriminate interspecific differences.We constructed a sequence database of the internal transcribed spacer (ITS)1-5.8S-ITS2 region for 196 Dendrobium species, all of which were identified accurately based on the morphological characters of vegetative and reproductive organs. The database covers most of the Dendrobium species from China and adjacent regions. The sequence readout of ITS1 and ITS2 regions of nuclear ribosomal DNA appeared to contain sufficient variation to discriminate Dendrobium at the interspecific level...

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“…The ARMS method, on the other hand, could be applied also for P. emblica with simplicity and efficiency. 24) The nucleotide sequence of the ITS region of P. emblica was aligned and searched for different sites for designing the specific primer. The result showed that amplicon of 497 bp was amplified by PHE.Bf/PHE.Br only for P. emblica (Fig.…”

Section: Authentication Of Medicinal Terminalia Species Their Crude mentioning

confidence: 99%

“…[19][20][21][22][23] The amplification refractory mutation system (ARMS), an easy and cost-effective method for authenticating herbs and their adulterants, is an allele-specific PCR that allows discrimination of alleles at specific loci differing by as little as 1 bp. 24) Both techniques are based on the generic variation of SNPs.…”

mentioning

confidence: 99%

Molecular Analysis of <i>Terminalia</i> spp. Distributed in Thailand and Authentication of Crude Drugs from <i>Terminalia</i> Plants

Intharuksa

Ando

Miyake

et al. 2016

Biological & Pharmaceutical Bulletin

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Terminalia, a large genus of Combretaceae, is distributed in Tropical Asia, Africa, and America. Some Terminalia plants are used in folk medicine because they possess powerful medicinal properties. Dried fruits of Terminalia bellirica and Terminalia chebula are used as the main ingredient in Triphala, a famous polyherbal formulation in Ayurvedic medicine and Thai folk medicine, because of their laxative, detoxifying, and rejuvenating effects. To clarify the phylogenetic relationships of medicinal Terminalia species (T. bellirica, T. chebula, and T. catappa) and authenticate their crude drugs, "Samo" and Triphala, nucleotide sequencing alignments in the internal transcribed spacer one-two (ITS 1-2) regions of Terminalia plants collected in Thailand were performed. The amplified fragments of Terminalia species were approximately 800 bp in length. To compare these sequences and DDBJ registered data, a molecular phylogenetic tree was constructed. Phylogenetic analysis clearly separated the sequences into two groups: Asian Terminalia and African Terminalia with some exceptions. In the analyzed sequences, the length of the ITS1-5.8S-ITS2 region was 674 bp in T. chebula, and 677 bp in T. bellirica and T. catappa. Eighty-one single nucleotide polymorphisms (SNPs) and nine insertion-deletions (indels) were observed, and the nucleotide sequences of this region showed species-specific sequences. Based on these differences, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation system (ARMS) were applied to identify medicinal Terminalia species. Moreover, the ARMS method was chosen for fingerprinting analysis of Samo crude drugs and Triphala formulations because it was a fast, cost-effective, and reproducible approach.

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SNP, ARMS and SSH Authentication of Medicinal Dendrobium officinale KIMURA et MIGO and Application for Identification of Fengdou Drugs

Cited by 24 publications

References 19 publications

Identification of medicinal Dendrobium species by phylogenetic analyses using matK and rbcL sequences

Identification of medicinal Dendrobium species by phylogenetic analyses using matK and rbcL sequences

Identification of Dendrobium Species Used for Herbal Medicines Based on Ribosomal DNA Internal Transcribed Spacer Sequence

Molecular Analysis of <i>Terminalia</i> spp. Distributed in Thailand and Authentication of Crude Drugs from <i>Terminalia</i> Plants

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