Molecular cloning and biochemical characterization of a Cu,Zn-superoxide dismutase from Scedosporium apiospermum

Hydroxamate siderophores of Scedosporium apiospermum

“…Only two putative virulence factors have been identified for S. apiospermum, i.e. an alkaline serine-protease belonging to the subtilisin subfamily (Larcher et al 1996) and a Cu, Zn superoxide dismutase (Lima et al 2007) which may help the fungus to invade the host tissues and to evade the host immune response, respectively.…”

“…This strain was previously used for purification and characterization of a subtilisin-like serine protease (Larcher et al 1996) and a Cu, Zn superoxide dismutase (Lima et al 2007) and it was deposited at the IHEM (Institute of Hygiene and Epidemiology-Mycology section) culture collection (Scientific Institute of Public Health, Brussels, Belgium) under the accession number 15155. According to recent taxonomic changes (Gilgado et al 2005(Gilgado et al , 2008, this strain was identified as S. apiospermum sensu stricto by sequencing of the internal transcribed sequence (ITS) regions (ITS1 and ITS2) of the RNAr genes.…”

“…For all these reasons, S. apiospermum is a challenging pathogen and there is an urgent need for a better understanding of the pathogenesis of scedosporiosis. Potential virulence factors have been identified in S. apiospermum, including a subtilisinlike serine-protease (Larcher et al 1996) and a Cu, Zn superoxide dismutase (Lima et al 2007). This study was therefore designed to identify siderophores in S. apiospermum since there is now accumulated evidence for their role as virulence factors in numerous pathogens.…”

Identification of Scedosporium boydii catalase A1 gene, a reactive oxygen species detoxification factor highly expressed in response to oxidative stress and phagocytic cells

“…Nevertheless, very few biochemical studies, focused on polysaccharides (a-glucans, rhamnomannans, and peptidorhamnomannans [PRMs]) ), glycolipids (glucosylceramides) (Pinto et al 2002), and siderophores (Bertrand et al 2009), have been performed so far regarding species of the S. apiospermum complex. In addition, hydrolytic enzymes thought to be involved in the host tissue invasion by the degradation of host protein barriers such as serine proteases (Larcher et al 1996), metalloproteases (Pereira et al 2009;Silva et al 2011), and phosphatases (Kiffer-Moreira et al 2007) have been identified, as well as enzymes possibly involved in evading the host oxidative response such as a cytosolic Cu,Zn-superoxide dismutase (SOD) (Lima et al 2007). In addition, we showed recently that S. boydii produces three distinct catalases and one of these enzymes called catalase A1 (Cat A1) was purified and characterized (Mina et al 2015).…”

Purification and characterization of Cu, Zn-superoxide dismutase from black soybean

“…SODs have been found in diverse types of organisms, involving animals (Ookawara et al, 1997;Öztürk-Ürek & Tarhan, 2001;Wang, Osatomi, Nagatomo, Yoshida, & Hara, 2011), plants (Nice & Robinson, 1993;Sheng, Zheng, & Tong, 2004;Vyas & Kumar, 2005), fungi (Bittencourt et al, 2004;Dolashka-Angelova, Angelova, Genova, Stoeva, & Voelter, 1999;Lima et al, 2007;Nedeva et al, 2009;Wang, He, Li, & Yuan, 2005) and bacteria (Benov, Beyer, Stevens, & Fridovich, 1996). According to the metallic cofactors, SODs are classified as Cu, Zn-SOD, Mn-SOD and Fe-SOD (Bowler, Camp, Montagu, & Inze, 1994).…”