Identification of Scedosporium boydii catalase A1 gene, a reactive oxygen species detoxification factor highly expressed in response to oxidative stress and phagocytic cells

Mina, Sara; Staerck, Cindy; D'Almeida, Sènan Mickael; Marot, Agnès; Delneste, Yves; Calenda, Alphonse; Tabiasco, Julie; Bouchara, Jean‐Philippe; Fleury, Maxime

doi:10.1016/j.funbio.2015.09.007

Cited by 16 publications

(11 citation statements)

References 62 publications

(60 reference statements)

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“…Study was conducted on the reference strain Scedosporium apiospermum IHEM 14462 originally isolated from a sputum sample from a CF patient and previously used for whole genome sequencing. 28 The fungus was maintained by weekly passages on potatoe-dextrose-agar (PDA, containing in g/L: dextrose, 20; infusion from potatoes, 200; and bacteriological agar, 15; CON-DALAB, Madrid, Spain) supplemented with chloramphenicol 0.5%, with incubation at 37 • C. Conidia from 9-day-old cultures collected by scraping as previously described 34 were inoculated in yeast extract-peptone-dextrose (YPD; containing in g/L: yeast extract, 10; peptone, 20; glucose, 20; and chloramphenicol 0.5%, pH 7) broth in Petri dishes (30 mL per dish; 2 × 10 6 conidia per mL), which were incubated for 24 h at 37 • C to obtain hyphae.…”

Section: Fungal Strain and Growth Conditionsmentioning

confidence: 99%

“…Co-culture experiments were realized as previously described 34 with slight modifications. The human myelomonocytic leukemia cell line THP-1 (ATCC) was cultivated in Roswell Park Memorial Institute (RPMI) 1640 medium, and the human promyelocytic cell line HL60 (ATCC) in IMDM.…”

Section: Co-cultivation With Phagocytic Cellsmentioning

confidence: 99%

“…33 Genes encoding SODC and CatA1 were sequenced, and it was demonstrated that CatA1 gene was highly expressed in response to the oxidative stress. 34 Recently, a genome-wide analysis in S. apiospermum permitted to identify 31 other genes encoding proteins putatively implicated in ROS or RNS detoxification. 35 In order to get a better knowledge of the pathogenic mechanisms of Scedosporium species, we investigated here the transcriptional response of these genes to chemical oxidative stresses and in co-culture with activated phagocytic cells.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional profiling ofScedosporium apiospermumenzymatic antioxidant gene battery unravels the involvement of thioredoxin reductases against chemical and phagocytic cells oxidative stress

et al. 2018

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Scedosporium species rank the second, after Aspergillus fumigatus, among the filamentous fungi colonizing the airways of patients with cystic fibrosis (CF). Development of microorganisms in the respiratory tract depends on their capacity to evade killing by the host immune system, particularly through the oxidative response of macrophages and neutrophils, with the release of reactive oxygen species (ROS) and reactive nitrogen species (RNS). This is particularly true in the airways of CF patients which display an exacerbated inflammatory reaction. To protect themselves, pathogens have developed various enzymatic antioxidant systems implicated in ROS degradation, including superoxide dismutases, catalases, cytochrome C peroxidases, chloroperoxidases and enzymes of the glutathione and thioredoxin systems, or in RNS degradation, that is, flavohemoglobins, nitrate reductases, and nitrite reductases. Here we investigated the transcriptional regulation of the enzymatic antioxidant gene battery in 24-h-old hyphae of Scedosporium apiospermum in response to oxidative stress induced chemically or by exposure to activated phagocytic cells. We showed that 21 out of the 33 genes potentially implicated in the oxidative or nitrosative stress response were overexpressed upon exposure of the fungus to various chemical oxidants, while they were only 13 in co-cultures with macrophages or neutrophils. Among them, genes encoding two thioredoxin reductases and to a lesser extent, a peroxiredoxin and one catalase were found to be overexpressed after chemical oxidative stress as well as in co-cultures. These results suggest that thioredoxin reductases, which are known to be virulence factors in other pathogenic fungi, play a key role in pathogenesis of scedosporiosis, and may be new drug targets.

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Section: Fungal Strain and Growth Conditionsmentioning

confidence: 99%

Section: Co-cultivation With Phagocytic Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptional profiling ofScedosporium apiospermumenzymatic antioxidant gene battery unravels the involvement of thioredoxin reductases against chemical and phagocytic cells oxidative stress

et al. 2018

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“…Besides, upregulated oxidative phosphorylation in cases could lead to the accumulation of reactive oxygen species (Fig. 3), possibly resulting in systemic damage and inflammation [48], as corroborated by the measured high expression of genes coding for ROS-degrading enzymes and phospholipases.…”

Section: Discussionmentioning

confidence: 65%

Integrative analyses of TEDDY Omics data reveal lipid metabolism abnormalities, increased intracellular ROS and heightened inflammation prior to autoimmunity for type 1 diabetes

et al. 2021

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Background The Environmental Determinants of Diabetes in the Young (TEDDY) is a prospective birth cohort designed to study type 1 diabetes (T1D) by following children with high genetic risk. An integrative multi-omics approach was used to evaluate islet autoimmunity etiology, identify disease biomarkers, and understand progression over time. Results We identify a multi-omics signature that was predictive of islet autoimmunity (IA) as early as 1 year before seroconversion. At this time, abnormalities in lipid metabolism, decreased capacity for nutrient absorption, and intracellular ROS accumulation are detected in children progressing towards IA. Additionally, extracellular matrix remodeling, inflammation, cytotoxicity, angiogenesis, and increased activity of antigen-presenting cells are observed, which may contribute to beta cell destruction. Our results indicate that altered molecular homeostasis is present in IA-developing children months before the actual detection of islet autoantibodies, which opens an interesting window of opportunity for therapeutic intervention. Conclusions The approach employed herein for assessment of the TEDDY cohort showcases the utilization of multi-omics data for the modeling of complex, multifactorial diseases, like T1D.

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“…Recently, the potential value of a S. boydii antigen, mycelial catalase A1, in serodiagnosis of a Scedosporium infection was investigated by ELISA, using sera from CF patients. 94,95 Whatever the Scedosporium species involved (including S. boydii, S. apiospermum and S. aurantiacum), sera from Scedosporium-infected patients were clearly differentiated from sera from A. fumigatus-infected CF patients. The assay exhibited a 100% sensitivity and a very high specificity (97.44%), with discordant results obtained for only one patient.…”

Section: Serodiagnosis As a Mean To Differentiate Airway Colonization From Respiratory Infectionmentioning

confidence: 99%

Developing collaborative works for faster progress on fungal respiratory infections in cystic fibrosis

et al. 2018

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Cystic fibrosis (CF) is the major genetic inherited disease in Caucasian populations. The respiratory tract of CF patients displays a sticky viscous mucus, which allows for the entrapment of airborne bacteria and fungal spores and provides a suitable environment for growth of microorganisms, including numerous yeast and filamentous fungal species. As a consequence, respiratory infections are the major cause of morbidity and mortality in this clinical context. Although bacteria remain the most common agents of these infections, fungal respiratory infections have emerged as an important cause of disease. Therefore, the International Society for Human and Animal Mycology (ISHAM) has launched a working group on Fungal respiratory infections in Cystic Fibrosis (Fri-CF) in October 2006, which was subsequently approved by the European Confederation of Medical Mycology (ECMM). Meetings of this working group, comprising both clinicians and mycologists involved in the follow-up of CF patients, as well as basic scientists interested in the fungal species involved, provided the opportunity to initiate collaborative works aimed to improve our knowledge on these infections to assist clinicians in patient management. The current review highlights the outcomes of some of these collaborative works in clinical surveillance, pathogenesis and treatment, giving special emphasis to standardization of culture procedures, improvement of species identification methods including the development of nonculture-based diagnostic methods, microbiome studies and identification of new biological markers, and the description of genotyping studies aiming to differentiate transient carriage and chronic colonization of the airways. The review also reports on the breakthrough in sequencing the genomes of the main Scedosporium species as basis for a better understanding of the pathogenic mechanisms of these fungi, and discusses treatment options of infections caused by multidrug resistant microorganisms, such as Scedosporium and Lomentospora species and members of the Rasamsonia argillacea species complex.

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Identification of Scedosporium boydii catalase A1 gene, a reactive oxygen species detoxification factor highly expressed in response to oxidative stress and phagocytic cells

Cited by 16 publications

References 62 publications

Transcriptional profiling ofScedosporium apiospermumenzymatic antioxidant gene battery unravels the involvement of thioredoxin reductases against chemical and phagocytic cells oxidative stress

Transcriptional profiling ofScedosporium apiospermumenzymatic antioxidant gene battery unravels the involvement of thioredoxin reductases against chemical and phagocytic cells oxidative stress

Integrative analyses of TEDDY Omics data reveal lipid metabolism abnormalities, increased intracellular ROS and heightened inflammation prior to autoimmunity for type 1 diabetes

Developing collaborative works for faster progress on fungal respiratory infections in cystic fibrosis

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