Hydrogels with high strength and ductility are normally prepared from synthetic polymers, and most protein-based hydrogels are soft and brittle. Here, a strong, ductile gelatin hydrogel is prepared by simply soaking a virgin gelatin gel in an ammonium sulfate solution. The polymer chains in the covalent, crosslink-free network can freely move to homogeneously distribute stress, and more importantly, the highly kosmotropic ammonium sulfate ions greatly enhance the hydrophobic interactions and chain bundling within the gelatin gels. As a result, the treated hydrogels have an extraordinary ultimate strength (compressive and tensile strains of over 99% and 500%, respectively, and stresses of 12 and 3 MPa) superior to that of common protein gels. The physical crosslinks introduced by the Hofmeister effect can rapidly absorb energy and sustain large deformations via decrosslinking and dissociation, which result in energy dissipation and antifatigue properties. The effects of the gelatin and (NH 4 ) 2 SO 4 concentrations on the hydrogel mechanics are evaluated, and the possible strengthening mechanism is discussed. The effect of various ions in the Hofmeister series on the gelatin hydrogel is also investigated. Kosmotropic ions enhance the mechanical properties, whereas chaotropic ions soften and dissolve the gel.

N6-methyladenosine (m6A), the most prevalent modification of mammalian RNA, has received increasing attention. Although m6A has been shown to be associated with biological activities, such as spermatogenesis modulation, cell spermatogenesis and pluripotency, Drosophila sex determination, and the control of T cell homeostasis and response to heat shock, little is known about its roles in cancer biology and cancer stem cells. Recent articles have noted that some genes have abnormal m6A expression after tumorigenesis, including genes ABS2, RARA, MYB, MYC, ADAM19 and FOX1. Abnormal changes in the m6A levels of these genes are closely related to tumour occurrence and development. In this review, we summarized the ‘dual edge weapon’ role of RNA methylation in the tumorigenesis. We discussed RNA methylation could lead to not only tumour progression but also tumour suppression. Moreover, we clarified that the abnormal changes in the m6A enrichment of specific loci contribute to tumour occurrence and development, thereby representing a novel anti-cancer strategy by restoration to balanced RNA methylation in tumour cells.

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Using fluorescently labeled DNA oligonucleotides and nanomaterials for developing biosensors has been extensively reported for gold nanoparticles (AuNPs) and graphene oxide (GO) among others. These materials have vastly different affinities and mechanisms for interacting with DNA, and their analytical performance is likely to be different. In this work, we used several DNA sequences and, respectively, adsorbed them on AuNPs and GO to quench fluorescence. Different from previous work, we used KCN to fully dissolve the AuNPs to calculate the percentage of the desorbed DNA due to the complementary DNA (cDNA) and aptamer target. The desorbed probe DNA from the AuNPs was less than 5% for all of the targets including DNA, adenosine, Hg2+, and lysozyme, indicating a very strong DNA adsorption affinity. Desorption of DNA was achieved by adding HEPES buffer, NaCl, and As­(III), but such desorption was attributed to the adsorption of these molecules or ions by the AuNPs instead of their interaction with the adsorbed DNA. For GO, more probes desorbed with addition of target analytes but so did nonspecific desorption by random DNA and proteins. In summary, AuNPs are unlikely to be a good surface for developing biosensors relying solely on the desorption of probe DNA, while for GO the main problem is nonspecific desorption.