Molecular characterization of two novel Glu-D1-encoded subunits from Chinese wheat (Triticum aestivum L.) landrace and functional properties of flours possessing the two novel subunits

Abstract

The high molecular weight glutenin subunits (HMW-GS) can be used for wheat quality improvement. Two novel alleles (designated 1Dx1.5* and 1Dy12.2*, respectively) at the Glu-D1 locus were identified in the Chinese wheat landrace variety 'Jiuquanjinbaoyin' by comparison of subunit mobility with that previously identified in several standard hexaploid wheats. The 1Dx1.5* and 1Dy12.2* genes were isolated using the allele-specific PCR primers and the complete open reading frames (ORFs) were obtained. Allele 1Dx1.5* consists of 2487 bp encoding a mature protein of 827 amino acid residues, whereas 1Dy12.2* consists 1980 bp encoding 658 residues. Comparisons of amino acid sequences analysis showed that 1Dx1.5* had higher similarity with the HMW-GS isolated from the wheat related species (Aegilops tauchi Coss.) than from the bread wheat varieties (Triticum aestivum L.). The 1Dy12.2* amino acid sequence showed a generally similar to the 1Dy12* isolated from Chinese endemic wheats.

Effects of genotype and environment on grain yield and quality traits in bread wheat (T. aestivum L.)

“…Glu-D1d (5+10) is a HMW-GS allele conferring better quality (Guo et al, 2010). Positive effect of Glu-D1d was present on the genotypes used in this study.…”

Characterisation of a novel high-molecular-weight glutenin subunit 1Dy12.3 from hexaploid wheat (Triticum aestivum L.)

“…Another three HMWGSs were identified in hexaploid wheat (genome AABBDD), with two in T. aestivum L., the 1Dy12* subunit in the Slovak obsolete cultivar Trebišovská (GenBank accession: EU266533, Mihálik & Gregová, unpublished) and the subunit 1Dy12.2* in the Chinese landrace Jiuquanjinbaoyin (GenBank: FJ226583, Guo et al 2010), and the third subunit (1Dy10.1) in T. petropavlovskyi Udacz. et Migush (GenBank: AY695379, Jiang et al 2006).…”

Isolation and molecular characterization of two novel HMW-GS genes from Chinese wheat (Triticum aestivum L.) landrace Banjiemang

“…Different resistance and tolerance genes as well as glutenin genes related to quality from the landraces are found (Guo et al 2010;Lan et al 2013;Xiao et al 2013a, b). As a result, these wheat landraces can be exploited in wheat cultivar improvement programmes.…”

“…The subunits 1Ax2*, 1Dx5?1Dy10, 1Bx17?1By18 are positively associated with bread-making qualities in contrast with null, 1Dx2?1Dy12, 1Bx7?1By8 (Branlard and Dardevet 1985;Payne 1987;Rogers et al 1989). The characterization of such quality related to HMW subunits in bread wheat leads to searching for new alleles of HMW-GS in common wheat and its related species (Wan et al 2002;Wang et al 2004;Cong et al 2007;Pang and Zhang 2008;Guo et al 2010;Jiang et al 2005Jiang et al , 2010Jin et al 2012;Ma et al 2013;Xiao et al 2014). Considerable numbers of studies showed that alleles of HMW-GS at one individual locus are rich in Chinese wheat landraces.…”

“…Zheng et al (2011) detected 22 alleles (3 at the Glu-A1, 13 at the Glu-B1 and 6 at the Glu-D1) in 485 common landraces from the reaches of the Yangtze-River. Chinese wheat landraces are valuable resources of which some useful HMW alleles can be exploited to improve wheat quality (Guo et al 2010). To exploit potentially new resources of HMW-GS genes usable for improving wheat end-use qualities, the study isolated and characterized two HMW glutenin genes at Glu-B1 locus in Banjiemang hereto unknown in wheat and other Triticeae species.…”