Molecular characterization of the first non-mammalian p73 cDNA

Expression of homologues for p53 and p73 in the softshell clam (Mya arenaria), a naturally-occurring model for human cancer

“…While p53 family members exist throughout the mammals, homologues for p73 in other vertebrates have only been reported for Barbus barbus (a cyprinid ®sh: Accession #AF043641; Bhaskaran et al, 2000). In the invertebrates, a p73-like gene has been identi®ed for Loligo forbesi (squid; Accession #U43595), a p53-like gene has been identi®ed and its role in apoptosis characterized for Drosophila melanogaster (fruit¯y; Brodsky et al, 2000;Ollmann et al, 2000;Steller, 2000) and previously, partial p53 sequences were identi®ed for M. arenaria (Barker et al, 1997;Accession #U45237 and #U45238).…”

Fish Ecogenotoxicology: An Emerging Science, an Emerging Tool for Environmental Monitoring and Risk Assessment

“…This will help in formulating longterm strategies for fish conservation programme besides estimating safe Level of pollutants in water [32]. Recent advancement has been made in the field of ecogenotoxicology [11,72,[103][104][105], which use has also been recommended for in genotoxicity testing of new chemical entity (NCE) and pharmaceuticals by the International Conference on Harmonization (ICH) [66]. However, several drawbacks have hindered the effective use of genotoxicity tests in ecogenotoxicology [7,13,30,66].…”

“…Winn et al [103] prepared a transgenic fish that carries multiple copies of a bacteriophage lambda vector that harbours the cII gene as a mutational target, a technique originally developed for lambda transgenic rodents. The p53 tumor suppressor gene, which is known to be implicated in cancer development, has been investigated as a possible biomarker for genotoxin in fish cells [11,104,105]. The amplification of DNA by polymerase chain reaction technique enabled the detection of mutations at specific sites and the development of electrochemical DNA based biosensors [106,107].…”

An introduction about Genotoxicology Methods as Tools for Monitoring Aquatic Ecosystem: Present status and Future perspectives

“…Another transgenic fish, that bikes multiple copies of a bacteriophage lambda vector harboring the cII gene as a mutational target, has been established by a technique originally developed for lambda transgenic rodents [133]. The p 53 tumor suppressor gene, which is known to be implicated in cancer development, has been investigated as a possible biomarker for genotoxin in fish cells [134].…”