Expression of homologues for p53 and p73 in the softshell clam (Mya arenaria), a naturally-occurring model for human cancer

Kelley, Melissa L.; Winge, Per; Heaney, Jason D.; Stephens, R. E.; Farell, Julianne H; Beneden, Rebecca J. Van; Reinisch, Carol L.; Lesser, Michael P.; Walker, Charles W.

doi:10.1038/sj.onc.1204144

Cited by 86 publications

(112 citation statements)

References 58 publications

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“…However, no N-terminal extension was detected in Mytilus when compared to Mya. In M. arenaria, a N-terminal extension was detected 8 aa distance upstream from the conventional start site which leads to a potential second start site for protein translation (Kelley et al, 2001).…”

Section: Rt-pcrmentioning

confidence: 99%

“…Other authors (Krishnakumar et al, 1999) were unable to link chemical exposure to onset of haemic neoplasia. We and others have demonstrated that the p53 gene and its family member, p73, are implicated in the onset of molluscan leukemia (Barker et al, 1997;Kelley et al, 2001;Stephens et al, 2001). In the softshell clam Mya arenaria, mutations occur in the p53 transcript of leukemic versus normal haemocytes (Barker et al, 1997).…”

Section: Introductionmentioning

confidence: 95%

“…In the softshell clam Mya arenaria, mutations occur in the p53 transcript of leukemic versus normal haemocytes (Barker et al, 1997). In addition, transcription of the p53 gene family member p73 is up-regulated in leukemic haemocytes of adult M. arenaria (Kelley et al 2001) and p53 family member protein expression is altered in the transition of normal haemocytes to leukemia cells . In leukemic Mya arenaria haemocytes, p53 is located in the cytoplasm rather than in the nucleus as it is for normal cells, indicating that p53 is functionally altered in leukemic haemocytes (Kelley et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Kelley et al (2001) isolated p53 and p73 from the bivalve mollusc Mya arenaria and a partial sequence for a p53 is available for the oyster Crassostrea rhizophorae (Genbank Accession Number AY442309).…”

Section: Introductionmentioning

confidence: 99%

“…Several authors have used various monoclonal and polyclonal antibodies raised against human and clam (Spisula, Mya) p53 family members to study the expression of the p53 family and to distinguish leukemic cells from normal cells (Kelley et al, 2001;Stephens et al, 2001;Jessen-Eller et al, 2002;Cox et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Identification and phylogenetic comparison of p53 in two distinct mussel species (Mytilus)

Muttray

Cox

St-Jean

et al. 2005

Comparative Biochemistry and Physiology Part C: Toxicology &

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The extent to which humans and wildlife are exposed to anthropogenic challenges is an important focus of environmental research. Potential use of p53 gene family marker(s) for aquatic environmental effects monitoring is the long-term goal of this research. The p53 gene is a tumor suppressor gene that is fundamental in cell cycle control and apoptosis. It is mutated or differentially expressed in about 50 % of all human cancers and p53 family members are differentially expressed in leukemic clams. Here we report the identification and characterization of the p53 gene in two species of Mytilus, Mytilus edulis and Mytilus trossulus, using RT-PCR with degenerate and specific primers to conserved regions of the gene. The Mytilus p53 proteins are 99.8 % identical and closely related to clam (Mya) p53. In particular, the 3' untranslated regions were examined to gain understanding of potential post-transcriptional regulatory pathways of p53 expression. We found nuclear and cytoplasmic polyadenylation elements, adenylate/uridylate-rich elements, and a K-box motif previously identified in other, unrelated genes. We also identified a new motif in the p53 3'UTR which is highly conserved across vertebrate and invertebrate species. Differences between the p53 genes of the two Mytilus species may be part of genetic determinants underlying variation in leukemia prevalence and/or development, but this requires further investigation. In conclusion, the conserved regions in these p53 paralogues may represent potential control points in gene expression. This information provides a critical first step in the evaluation of p53 expression as a potential marker for environmental assessment.

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Section: Rt-pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Identification and phylogenetic comparison of p53 in two distinct mussel species (Mytilus)

Muttray

Cox

St-Jean

et al. 2005

Comparative Biochemistry and Physiology Part C: Toxicology &

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Evolution of the p53 and p63/p73 Gene Families

Pintus

2009

Encyclopedia of Life Sciences

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The tumour suppressor protein p53 is one of the most studied eukaryote proteins. The finding of its paralogues – p63 and p73 – inspired investigations of the whole family. p73 and p63 appeared to have functions distinct from p53 and to be involved in complex regulation of the latter. Evolutionary studies revealed distant homologues of the family not only in vertebrates but also in protostomes and even in nonmetazoans. The evolutionary history of the p53 family and its relatives is complex, controversial and its intensive study goes on. Key concepts: The p53 and p63/p73 gene families evolve from the common p63‐like ancestor via specialization through gene duplications and domain gains and losses.

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Oxidative Challenge and Redox Sensing in Mollusks: Effects of Natural and Anthropic Stressors

Letendre

Leboulenger

Durand

2011

Oxidative Stress in Vertebrates and Invertebrates

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Expression of homologues for p53 and p73 in the softshell clam (Mya arenaria), a naturally-occurring model for human cancer

Cited by 86 publications

References 58 publications

Identification and phylogenetic comparison of p53 in two distinct mussel species (Mytilus)

Identification and phylogenetic comparison of p53 in two distinct mussel species (Mytilus)

Evolution of the p53 and p63/p73 Gene Families

Oxidative Challenge and Redox Sensing in Mollusks: Effects of Natural and Anthropic Stressors

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