Oxidative Challenge and Redox Sensing in Mollusks: Effects of Natural and Anthropic Stressors

Letendre, Julie; Leboulenger, F.; Durand, Fabien

doi:10.1002/9781118148143.ch26

Cited by 8 publications

(5 citation statements)

References 121 publications

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“…Exposure to ZnO NPs (0.12 µM), low levels (0.12 µM) of ionic Zn and high concentration (1.2 µM) of suspended NRs led to a slight but significant increase in the total levels of cathepsin D and a notable increase in the free cathepsin D levels. The increase in free cathepsin D was associated with a decline in the lysosomal membrane stability (measured in hemocytes as a key cell type integrating the organismal stress [91,92]) in the mussels from the same exposure groups, consistent with the cathepsin D release from lysosomes. Activation of autophagic enzymes, such as cathepsin D, in mussels might indicate elevated recycling of intracellular structures to augment energy supply [70] and/or their increased breakdown to minimize stress-induced cellular damage [40,93].…”

Section: Lysosomal Responses To Zn-containing Exposures In Musselssupporting

confidence: 55%

The effects of ZnO nanostructures of different morphology on bioenergetics and stress response biomarkers of the blue mussels Mytilus edulis

Falfushynska

et al. 2019

Science of The Total Environment

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Biofouling causes massive economical losses in maritime sector creating urgent need for effective and ecologically non-harmful antifouling materials. Zinc oxide (ZnO) nanorod coatings show promise as an antifouling material; however, their toxicity to marine organisms is not known. We studied compared toxicity of suspended and immobilized ZnO nanorods (NR) with that of ZnO nanoparticles (NP) and ionic Zn 2+ in a marine bivalve Mytilus edulis. Multi-biomarker assessment included bioenergetics markers (tissue energy reserves, activity of mitochondrial electron transport system and autophagic enzymes), expression of apoptotic genes, and general stress biomarkers (oxidative lesions, lysosomal membrane stability and metallothionein expression) in the mussels. All Zn-containing exposures led to lysosomal membrane destabilization in hemocytes, but no strong disturbance of the wholeorganism energy status. Exposure to NP and NR caused accumulation of oxidative lesions in proteins and lipids, stimulated autophagy, and led to overexpression of apoptosis-and inflammation-related genes. No induction of apoptotic or inflammatory pathways was seen in Zn 2+ exposures. Unlike ionic Zn 2+ , ZnO NP and NR share similar toxic mechanisms with strong pro-apoptotic and inflammatory signatures, but the NR toxicity is less than that of the NP at the same concentrations. Therefore, ZnO NRs have potential as low toxicity antifouling coatings in marine environments.

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Section: Lysosomal Responses To Zn-containing Exposures In Musselssupporting

confidence: 55%

The effects of ZnO nanostructures of different morphology on bioenergetics and stress response biomarkers of the blue mussels Mytilus edulis

Falfushynska

et al. 2019

Science of The Total Environment

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“…An imbalance in pro‐oxidant and antioxidant factors if sustained, results in oxidative stress, which is a precursor to altered gene expression, enzyme inactivation, disintegration of protein–lipid complexes, a possible loss of cellular structure and consequently cell death (Halliwell & Gutteridge, ; Livingstone, ). Studies have also demonstrated that the total antioxidant potentials of an organism could express organ‐specific, species‐specific or even population‐specific patterns for species living in heterogeneous habitats (Camus, Gulliksen, Depledge, & Jones, ; Letendre, Leboulenger, & Durand, ). For instance, the fluctuating environmental conditions (for example oxygen availability) that characterize the inter‐tidal zone could trigger the production of free oxygen radicals in tissues of invertebrates inhabiting the area; such environmental scenarios have been linked to the high levels of antioxidant activity observed in invertebrates residing in the inter‐tidal zone (Letendre et al, ; Malanga, Estevez, Calvo, & Puntarulo, ; In essence a number of factors determine the physiological requirements for survival and the extent to which different populations experience oxidative stress; this in turn can impinge on the health and fitness of animal populations.…”

Section: Introductionmentioning

confidence: 99%

Sex‐specific affinity for redox‐active metals influences antioxidant responses of Callinectes amnicola (blue crab) populations in littoral and open water habitats of a tropical coastal lagoon

Jerome

Hassan

Chukwuka³

2017

Marine Ecology

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The critical role of ecological preferences and opportunity in determining contaminant uptake and adaptive responses of sexes in the wild is still poorly understood. This ecological relationship was investigated by measuring metal bioaccumulation and antioxidant activity in male and female blue crab populations from open water habitat and the littoral/inter‐tidal zone of the Lagos Lagoon. A total of 741 samples of blue crab (littoral zone: 263 females, 137 males; open water zone, 230 females, 111 males) was collected monthly over 24 months (January 2010–January 2012) from each site and the measurements of morphometric features (wet weight, carapace length, carapace width) were recorded; condition index, metal (redox active: Cu, Zn, redox inactive: Pb, Cd) concentration in tissues (gills, hepatopancreas, gonads and muscle) and antioxidant activity (superoxide dismutase, reduced glutathione, glutathione peroxidase, catalase and malondialdehyde) were measured for each sex. Monthly sediment samples for both habitats were also analysed for metals using standard methods. Female crabs were significantly larger (p < .05) with a better condition index than the male crabs across sites and seasons, while higher oxidative damage was recorded in male crabs in the littoral zone compared to the open water zone. The results show that there was a negative association between antioxidant activity and lipid peroxidation; a negative relationship between concentrations of redox‐inactive metals (Pb and Cd) and antioxidant activity in male crab tissues; and a positive relationship between uptake of a redox‐active metal (Cu) and antioxidant activity in female crab tissues. Although these trends suggest sex‐specific toxicity, they also associate redox‐inactive metals with the downregulation of antioxidant activity and oxidative stress. Furthermore, the higher condition index of females corroborates the possibility of sex‐specific toxicity, while the larger‐sized females compared to males suggests size‐sexual dimorphism in the blue crab populations. The site‐specific oxidative damage between sexes may be attributed to the different complexity of both habitats, which affords different ecological opportunities for the sexes.

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“…It is likely that hypoxia triggers the production of NO, which, in NO-ergic neurons of the CNS in mytilids (animals resistant to hypoxia), is involved in a range of processes related to respiration. Such processes include the inhibition of the mitochondrial respiratory pathway and switching neurons from aerobic respiration to glycolysis under conditions of reduced intracellular oxygen concentrations, a process that minimizes the production of reactive oxygen species in the cell [104,105]. This hypothesis has been confirmed by experimental studies on the molecular mechanisms of hypoxia resistance in mytilids [105,106] and Arctica islandica [104].…”

Section: Unos In Bivalvesmentioning

confidence: 84%

Effect of Air Exposure-Induced Hypoxia on Neurotransmitters and Neurotransmission Enzymes in Ganglia of the Scallop Azumapecten farreri

Kotsyuba

Dyachuk

2022

IJMS

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The nervous system expresses neuromolecules that play a crucial role in regulating physiological processes. Neuromolecule synthesis can be regulated by oxygen-dependent enzymes. Bivalves are a convenient model for studying air exposure-induced hypoxia. Here, we studied the effects of hypoxia on the expression and dynamics of neurotransmitters, and on neurotransmitter enzyme distribution, in the central nervous system (CNS) of the scallop Azumapecten farreri. We analyzed the expression of the neurotransmitters FMRFamide and serotonin (5-HT) and the choline acetyltransferase (CHAT) and universal NO-synthase (uNOS) enzymes during air exposure-induced hypoxia. We found that, in early-stage hypoxia, total serotonin content decreased in some CNS regions but increased in others. CHAT-lir cell numbers increased in all ganglia after hypoxia; CHAT probably appears de novo in accessory ganglia. Short-term hypoxia caused increased uNOS-lir cell numbers, while long-term exposure led to a reduction in their number. Thus, hypoxia weakly influences the number of FMRFamide-lir neurons in the visceral ganglion and does not affect peptide expression in the pedal ganglion. Ultimately, we found that the localization and level of synthesis of neuromolecules, and the numbers of cells expressing these molecules, vary in the scallop CNS during hypoxia exposure. This indicates their possible involvement in hypoxia resistance mechanisms.

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Oxidative Challenge and Redox Sensing in Mollusks: Effects of Natural and Anthropic Stressors

Cited by 8 publications

References 121 publications

The effects of ZnO nanostructures of different morphology on bioenergetics and stress response biomarkers of the blue mussels Mytilus edulis

The effects of ZnO nanostructures of different morphology on bioenergetics and stress response biomarkers of the blue mussels Mytilus edulis

Sex‐specific affinity for redox‐active metals influences antioxidant responses of Callinectes amnicola (blue crab) populations in littoral and open water habitats of a tropical coastal lagoon

Effect of Air Exposure-Induced Hypoxia on Neurotransmitters and Neurotransmission Enzymes in Ganglia of the Scallop Azumapecten farreri

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