Molecular Characterization of the Equine Herpesvirus 1 Strains RacL11 and Kentucky D

Ghanem, Yasser M.; Ibrahim, Eman; Yamada, Souichi; Matsumura, Tomio; Osterrieder, Nikolaus; Yamaguchi, Tsuyoshi; Fukushi, Hideto

doi:10.1292/jvms.69.573

Cited by 4 publications

(2 citation statements)

References 21 publications

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“…In contrast to the attenuated EHV-1 strains (KyA, KyD and RacL11) used as vaccine, drastic mutations in gD sequence, such as deletion, inversion, and insertion, were not found in the strains here analyzed ( ). A possible explanation for DNA mutations in KyA, KyD and RacL11 strains may be due to serial passage in hamsters and culture cells ( Molinkova et al , 2004 ; Ghanem et al , 2007 ).…”

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confidence: 99%

Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers

Mori¹,

Lara²,

Cunha³

et al. 2015

Braz. J. Microbiol.

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Partial nucleotide sequences of ORF72 (glycoprotein D, gD), ORF64 (infected cell protein 4, ICP4) and ORF30 (DNA polymerase) genes were compared with corresponding sequences of EHV-1 reference strains to characterize the molecular variability of Brazilian strains. Virus isolation assays were applied to 74 samples including visceral tissue, total blood, cerebrospinal fluid (CSF) and nasal swabs of specimens from a total of 64 animals. Only one CSF sample (Iso07/05 strain) was positive by virus isolation in cell culture. EHV-1 Iso07/05 neurologic strain and two abortion visceral tissues samples (Iso11/06 and Iso33/06) were PCR-positive for ORF33 (glycoprotein B, gB) gene of EHV-1. A sequence analysis of the ORF72, ORF64 and ORF30 genes from three EHV-1 archival strains (A3/97, A4/72, A9/92) and three clinical samples (Iso07/05, Iso11/06 and Iso33/06) suggested that among Brazilian EHV-1 strains, the amplified region of the gD gene sequence is highly conserved. Additionally, the analysis of ICP4 gene showed high nucleotide and amino acid identities when compared with genotype P strains, suggesting that the EHV-1 Brazilian strains belonged to the same group. All the EHV-1 Brazilian strains were classified as non-neuropathogenic variants (N752) based on the ORF30 analysis. These findings indicate a high conservation of the gD-, ICP4- and ORF30-encoding sequences. Different pathotypes of the EHV-1 strain might share identical genes with no specific markers, and tissue tropism is not completely dependent on the gD envelope, immediate-early ICP4 and DNA polymerase proteins.

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confidence: 99%

Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers

Mori¹,

Lara²,

Cunha³

et al. 2015

Braz. J. Microbiol.

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“…This point mutation does not affect the species-specific immunogenic epitopes located in the C-terminal part of glycoprotein G (Crabb and Studdert 1993). In other genome sections under observation the Prostejov isolate does not vary from the reference strain V592 which proves the conservation of the genome of the EHV-1 virus; similarly to the absolute match in the sequences of parts of genes for gE and gI (Ghanem et al 2007). In monitoring the stability of our isolate during the in vitro passage on cell line RK13 (6 passages) we discovered a single point mutation which differentiates the sequence of the passaged virus from the sequence of the field isolate in the section encoding gC.…”

Section: Discussionmentioning

confidence: 56%

Sequence analysis of selected nucleotide sequences of abortogenic isolate of Equine Herpesvirus 1 and changes caused by serial passage in vitro

Molínková

2012

Acta Vet. Brno

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The aim of this work was to isolate the abortogenic virus strain of Equine Herpesvirus 1 representing the current infection situation in the Czech Republic, describe it at the molecular level with accent on genes coding viral glycoproteins and observe the changes caused by the passaging of the virus on a cell culture. In 2009, an isolate of equine herpesvirus 1 was obtained from an abortion case in a mare from a herd affected by abortion storm. The virus identification was performed using the PCR method. The virus was isolated on the RK 13 cell line and after 6 passages in vitro the stability of sequences of selected sections of the virus genome was assessed and compared with the original field isolate. The virus sequences were also compared with known sequences of the abortogenic reference virus strain (V592) and with other known viral strains. One point mutation in a nucleotide sequence coding glycoprotein G was found, distinguishing the field isolate from V592. One point mutation in the gene for glycoprotein C was passage-induced. It was noted that the virus during the passage on RK13 cell line in the monitored sections was stable and is a suitable starting material for next experiments. EHV-1, viral abortion in mares, glycoproteins, genom

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Complete genome sequence of virulent duck enteritis virus (DEV) strain 2085 and comparison with genome sequences of virulent and attenuated DEV strains

et al. 2011

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Molecular Characterization of the Equine Herpesvirus 1 Strains RacL11 and Kentucky D

Cited by 4 publications

References 21 publications

Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers

Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers

Sequence analysis of selected nucleotide sequences of abortogenic isolate of Equine Herpesvirus 1 and changes caused by serial passage in vitro

Complete genome sequence of virulent duck enteritis virus (DEV) strain 2085 and comparison with genome sequences of virulent and attenuated DEV strains

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