Complete genome sequence of virulent duck enteritis virus (DEV) strain 2085 and comparison with genome sequences of virulent and attenuated DEV strains

Wang, Jichun; Höper, Dirk W.; Beer, Martin; Osterrieder, Nikolaus

doi:10.1016/j.virusres.2011.07.004

Cited by 40 publications

(24 citation statements)

References 46 publications

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“…A significant difference may also be seen in arrangement of inverted and directly repeated sequences (Hayward et al 1975;Wadsworth et al 1976). Partial or complete genomic sequences of DEVs are rapidly accumulating and its analyses demonstrated that, although similar to other herpes viruses, the DEV genome also varies Wang et al 2011;Wu et al 2012a;Wu et al 2012b;Yang et al 2013). The Chinese DEV is a wild type virulent strain of DEV (Gao et al 2015).…”

Section: Etiologymentioning

confidence: 99%

Duck virus enteritis (duck plague) – a comprehensive update

Dhama

Kumar

Saminathan

et al. 2017

Veterinary Quarterly

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Duck virus enteritis (DVE), also called duck plague, is one of the major contagious and fatal diseases of ducks, geese and swan. It is caused by duck enteritis virus (DEV)/Anatid herpesvirus-1 of the genus Mardivirus, family Herpesviridae, and subfamily Alpha-herpesvirinae. Of note, DVE has worldwide distribution, wherein migratory waterfowl plays a crucial role in its transmission within and between continents. Furthermore, horizontal and/ or vertical transmission plays a significant role in disease spread through oral-fecal discharges. Either of sexes from varying age groups of ducks is vulnerable to DVE. The disease is characterized by sudden death, vascular damage and subsequent internal hemorrhage, lesions in lymphoid organs, digestive mucosal eruptions, severe diarrhea and degenerative lesions in parenchymatous organs. Huge economic losses are connected with acute nature of the disease, increased morbidity and mortality (5%-100%), condemnations of carcasses, decreased egg production and hatchability. Although clinical manifestations and histopathology can provide preliminary diagnosis, the confirmatory diagnosis involves virus isolation and detection using serological and molecular tests. For prophylaxis, both live-attenuated and killed vaccines are being used in broiler and breeder ducks above 2 weeks of age. Since DEV is capable of becoming latent as well as shed intermittently, recombinant subunit and DNA vaccines either alone or in combination (polyvalent) are being targeted for its benign prevention. This review describes DEV, epidemiology, transmission, the disease (DVE), pathogenesis, and advances in diagnosis, vaccination and antiviral agents/therapies along with appropriate prevention and control strategies.

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Section: Etiologymentioning

confidence: 99%

Duck virus enteritis (duck plague) – a comprehensive update

Dhama

Kumar

Saminathan

et al. 2017

Veterinary Quarterly

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show abstract

“…Currently, there are three complete genomic sequences of DEV strains available in GenBank: the Chinese virulent DEV strain (DEV CHv) [3, 4], the European virulent strain (2085) [5], and the attenuated vaccine strain (VAC) [6], and the publications related to the three genome sequences have cast light on the genome structure of DEV. DEV is a linear, double-stranded DNA virus, the genome size of which is approximately 158–162 kb [3–6].…”

Section: Findingmentioning

confidence: 99%

Duck enteritis virus (DEV) UL54 protein, a novel partner, interacts with DEV UL24 protein

Gao

Jia

Wang

et al. 2017

Virol J

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BackgroundUL24 is a multifunctional protein that is conserved among alphaherpesviruses and is believed to play an important role in viral infection and replication.ResultsIn this paper, to investigate putative UL24-binding proteins and to explore the functional mechanisms of DEV UL24, yeast two-hybrid (Y2H) was carried out, and further verified the interaction between UL24 and partners by co-immunoprecipitation and fluorescence microscopy experiments. Interaction partners of UL24 protein were screened by yeast two-hybrid (Y2H) with the cDNA library of DEV-CHv strain post-infection DEF cells. A novel partner, DEV UL54 protein, was discovered by Y2H screening and bioinformatic. Co-immunoprecipitation experiments suggested that DEV UL24 interacted with UL54 proteins. And distribution of a part of UL54 protein was changed from nucleus to cytoplasm in DF-1 cells of co-subcellular localization experiments which also showed that DEV UL24 interacted with UL54 proteins.ConclusionsThe interaction between the DEV UL24 and UL54 proteins was discovered for the first time. Thus, DEV UL54 protein as a novel partner interacted with DEV UL24 protein.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-017-0830-5) contains supplementary material, which is available to authorized users.

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“…The DEV genome is a linear, double-stranded DNA that is divided into a unique long region (UL) and a unique short region (US) flanked by a short internal repeat sequence (IRS) and a short terminal repeat sequence (TRS) 1 . The sequence of the complete DEV genome and the functions of several viral genes, except UL54, have been reported, which could help in eliminating DEV completely 2–4 .…”

Section: Introductionmentioning

confidence: 99%

Regulation of viral gene expression by duck enteritis virus UL54

Liu

Cheng

Wang

et al. 2017

Sci Rep

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Duck enteritis virus (DEV) UL54 is a homologue of human herpes simplex virus-1 (HSV-1) ICP27, which plays essential regulatory roles during infection. Our previous studies indicated that DEV UL54 is an immediate-early protein that can shuttle between the nucleus and the cytoplasm. In the present study, we found that UL54-deleted DEV (DEV-ΔUL54) exhibits growth kinetics, a plaque size and a viral DNA copy number that are significantly different from those of its parent wild-type virus (DEV-LoxP) and the revertant (DEV-ΔUL54 (Revertant)). Relative viral mRNA levels, reflecting gene expression, the transcription phase and the translation stage, are also significantly different between DEV-ΔUL54-infected cells and DEV-LoxP/DEV-ΔUL54 (Revertant)-infected cells. However, the localization pattern of UL30 mRNA is obviously changed in DEV-ΔUL54-infected cells. These findings suggest that DEV UL54 is important for virus growth and may regulate viral gene expression during transcription, mRNA export and translation.

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Complete genome sequence of virulent duck enteritis virus (DEV) strain 2085 and comparison with genome sequences of virulent and attenuated DEV strains

Cited by 40 publications

References 46 publications

Duck virus enteritis (duck plague) – a comprehensive update

Duck virus enteritis (duck plague) – a comprehensive update

Duck enteritis virus (DEV) UL54 protein, a novel partner, interacts with DEV UL24 protein

Regulation of viral gene expression by duck enteritis virus UL54

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